MSRE-HTPrimer

MSRE-HTPrimer is an open-source, portable, web-based and easy-to-use tool, which facilitates the design of primer pairs for epigenetic assay design and and genomic sequencing assay design. It uses a simple input and output model and can design primers for hundreds to thousands of target sequences in a single run. Moreover, it does not have any limitations on the number and size of target sequences. MSRE-HTPrimer provides significant improvements over existing solutions with following unique features: 1) visualization of primer pairs in UCSC genome browser, 2) search each resulting primer pair in UCSC In-Silico PCR database, 3) flexible primer selection and filtering based on custom quality matrix and 4) parallel primer design for several target sequences. The pipeline is equipped with multiprocessing capability and uses custom inputs and parameters to design specific primers.