#28 Error running mrsfast-ultra

[viktor]

Hi

I am trying to run mrsfast-ultra-3.3.0. I am getting this error
Error: Cannot Open the file __output__0__2 , when I try to run the program. This run absolutely fine with the example sequences you have provided. Please let me know how I can solve this problem.

Best Regards

[Faraz Hach]

Hi Vikto,

Thanks for your interest in mrsFAST-ultra. I will be needing a bit more information to track the issue you are facing.

1) Can you make sure you had enough space on your hard disk.
2) Send me the full command line and if you are using GUI, please let me know what parameters you set. Actually you can copy paste the full command line from GUI shell output.
3) If you dataset is public, please provide us the link to reference genome and data so we can try to re-produce the scenario.

Thanks
F

[viktor]

Hi Faraz

Thanks for the reply.
1) Yes, I checked it I have enough space ( more 3 TB).
2)command I used : mrsfast --search /home/viktor/softwares/mrsfast-ultra-3.3.0/genome_index/genome.fa --mem 400 --seq1 19_1_paired.fq --seq2 19_2_paired.fq --discordant-vh -e 4 --min 200 --max 500 --threads 40
3) Sorry, data is not public.

One thing can see is that the (Error: Cannot Open the file __output__0__2) the file (__output__0__2) is there in the folder, but why it is complaining then?. Can it be because I have 277,711 reference genome scaffolds as I tried it 100 scaffolds it works fine.

[Faraz Hach]

Please try version 3.3.1