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#84 Abundance scaled by FMG abundances?

New
MOCAT
FMG (1)
Medium
Defect
2018-04-05
2018-04-02
No

Hello,
Any advice for using the abundances for the 40 marker genes as a kind of baseline genomic abundance to correct for IGC gene abundances? I'm thinking of just dividing each IGC abundance by the average of these 40.
Thanks for your help,
Peter Bazeley

Related

Tickets: #84

Discussion

  • MOCAT

    MOCAT - 2018-04-02

    I would advise to use the mOTUs and the built in taxonomic pipeline for
    taxonomic abundances. I don't see the logic of mixing single gene
    abundances and taxonomic abundances.

    On Mon, Apr 2, 2018 at 17:30 Peter Bazeley theoark@users.sourceforge.net
    wrote:


    ** [tickets:#84] Abundance scaled by FMG abundances?**

    Status: New
    Labels: FMG
    Created: Mon Apr 02, 2018 03:30 PM UTC by Peter Bazeley
    Last Updated: Mon Apr 02, 2018 03:30 PM UTC
    Owner: MOCAT

    Hello,
    Any advice for using the abundances for the 40 marker genes as a kind of
    baseline genomic abundance to correct for IGC gene abundances? I'm thinking
    of just dividing each IGC abundance by the average of these 40.
    Thanks for your help,
    Peter Bazeley


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    Related

    Tickets: #84

  • Peter Bazeley

    Peter Bazeley - 2018-04-03

    Not for taxanomic comparison but as a way to deal with compositional bias. Wouldn't this give a kind of average gene abundane per species, or am I way off?

     
  • Peter Bazeley

    Peter Bazeley - 2018-04-05

    Hi. So in my quest to find abundances for the FMGs in each sample, it appears that there's no good way to associate COGs with the IGC catalog (or is there?). So the alternative appears to be to take the gene.prediction.assembly result sequences, extract the ones that were matched with the FMGs, and align those to the IGC catalog. Does that sound reasonable? Thanks, Peter

     
    • MOCAT

      MOCAT - 2018-04-06

      The IGC catalog has been annotated to eggNOG and about 20 other databases,
      which makes it possible to summarize gene abundances at all those levels.
      And this is done automatically by MOCAT in the functional step. Don't know
      what you mean by FMG.

      On Fri, Apr 6, 2018 at 00:10 Peter Bazeley theoark@users.sourceforge.net
      wrote:

      Hi. So in my quest to find abundances for the FMGs in each sample, it
      appears that there's no good way to associate COGs with the IGC catalog (or
      is there?). So the alternative appears to be to take the
      gene.prediction.assembly result sequences, extract the ones that were
      matched with the FMGs, and align those to the IGC catalog. Does that sound
      reasonable? Thanks, Peter


      ** [tickets:#84] Abundance scaled by FMG abundances?**

      Status: New
      Labels: FMG
      Created: Mon Apr 02, 2018 03:30 PM UTC by Peter Bazeley
      Last Updated: Tue Apr 03, 2018 12:43 AM UTC
      Owner: MOCAT

      Hello,
      Any advice for using the abundances for the 40 marker genes as a kind of
      baseline genomic abundance to correct for IGC gene abundances? I'm thinking
      of just dividing each IGC abundance by the average of these 40.
      Thanks for your help,
      Peter Bazeley


      Sent from sourceforge.net because you indicated interest in <
      https://sourceforge.net/p/mocat/tickets/84/>

      To unsubscribe from further messages, please visit <
      https://sourceforge.net/auth/subscriptions/>

      --
      Sent from my iPhone - apologies for any iCorrect mistakes.

       

      Related

      Tickets: #84


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