#32 Suspicious distribution of contig coverages

[Mariana Kluge]

Hello,

I'm using MIRA 4.9.5_2 to assemble Ion torrent reads for a viral metagenomic project and I'm having the same critical error with some of my assemblies:

MIRA warncode: CONCOV_SUSPICIOUS_DISTRIBUTION
Title: Suspicious distribution of contig coverages

0 contig(s) with a total of 0 bases (= -nan% of bases in all non-repetitive
large contigs) have an average coverage less than 75% of the average
coverage
of all non-repetitive large contigs.
0 contig(s) with a total of 0 bases (= -nan% of bases in all non-repetitive
contigs) have an average coverage more than 125% of the average coverage of
all non-repetitive large contigs.
0 contig(s) with a total of 0 bases (= -nan% of bases in all non-repetitive
contigs) have an average coverage 25% above or below the average coverage of
all non-repetitive large contigs.
Summary: found 3 indicator(s) for coverage problem(s).

I also tried to submit through IonGap but the contigs file keeps coming empty, probably due to the same issue.

Any clue of what it could be?

Thanks!