MutationGenerator

• Introduction
• Results
  • Statistics
• Material and Methods
  • Java code to generate the mutations
  • R script to plot the mutations

Introduction

We tested whether MimicrEE2 accurately introduces mutations. This has two componenents, was the mutation rate accurately simulated and b) do all sites have an equal chance of mutating.

We generated a population with 5 chromosomes (chr1=2500bp, chr2=1200bp, chr3=700bp, chr4=400bp, chr5=200bp) of size N=10 diploids and set the mutation rate mu=0.0002. In MimicrEE2 mutations are introduced directly before the gametes merge into a novel zygote. We thus simualted 250.000 times progeny for this population and counted the mutations occuring at each site. We expect that each site was mutated exactly 1000 times (mu2Nesites = 0.0002210*5000).

Results

Statistics

Chi-squared test for given probabilities

data: df$mutcount
X-squared = 5098.2, df = 4999, p-value = 0.1605

Material and Methods

Java code to generate the mutations

R script to plot the mutations