microsatelight-updates — Microsatelight public list

[Microsatelight-updates] Fwd: Error - Analysis not proceeding

[Andrew C. <cha...@gm...>]

---------- Forwarded message ----------
From: Andrew Chaulk <cha...@gm...>
Date: Thu, Mar 19, 2015 at 4:33 PM
Subject: Error - Analysis not proceeding
To: mic...@li...


Hello, I am new to Microsatelight and on my first run through the example
in the help file the process halted after selecting the folder where the
.fsa files are located. I attached an image with the script after the
program stopped running.

Any help would be greatly appreciated!

Thanks,

Andrew

[Microsatelight-updates] Error - Analysis not proceeding

[Andrew C. <cha...@gm...>]

Hello, I am new to Microsatelight and on my first run through the example
in the help file the process halted after selecting the folder where the
.fsa files are located. I attached an image with the script after the
program stopped running.

Any help would be greatly appreciated!

Thanks,

Andrew

[Microsatelight-updates] Assistance with running MICROSATELIGHT for the first time

[meytal m. <mey...@ya...>]

Hello,
 
I want
to try and use MICROSATELIGHT on my data. In order to learn how to use the
software, I'm trying to do the example with the provided test data. The first
step runs smoothly, and I'm able to create the “Sizing_Table.txt" file and to upload it into Option-2. Using Option-2, I've also been able to create
the “Selected.txt" file, but when I open it and view the text, I only see
the headlines (Sample File Name, Size, Height etc.). I don’t seem to get any
error messages and I'm not sure what I'm doing wrong. I will appreciate any
help on this subject.
 
Thanks,Meytal.            

 
Meytal Markovich
M.Sc Student
The Interuniversity Institute for Marine Sciences in Eilat 
P.O.B. 469 Eilat, 88103 
Israel 
Tel: +972-(0)8-6360179 
&
The Department of Life Sciences 
Marine Biology and Biotechnology 
Ben Gurion University; Beer Sheva; 
Israel 

[Microsatelight-updates] Problem running step two

[Duenas S. J. <Jua...@li...>]

Hi,



I am trialling MICROSATELIGHT to visualize microsatellites for my Master Thesis. IT department of the institution I study in installed successfully the program. R (R commander) and PeakScanner are installed on the computer as well.



When I get the program to run Step 2 (Peak Select), the program does not return any output. As the samples I got at the moment are singleplexes, I type "0" on the pop-up window that ask to specify the Multiplex group coding. When I click continue, nothing happens.



This is what the terminal line spits out:



          FLAGS = ()
          PV = 0x3797954 ""
          CUR = 0
          LEN = 20
Tk::Error: Usage $widget->destroy(...) at script/MICROSATELIGHT_v100_2.pl line 4
05.
 Tk callback for .frame.button1
 Tk::__ANON__ at Tk.pm line 250
 Tk::Button::butUp at Tk/Button.pm line 175
 <ButtonRelease-1>
 (command bound to event)
destroy (1):
 0 0x27b4c74 PVMG f=00e40807 {}(1)(3)
SV = PVMG(0x2d208fc) at 0x27b4c74
  REFCNT = 3
  FLAGS = (PADMY,GMG,SMG,RMG,ROK)
  IV = 0
  NV = 0
  RV = 0x375fe14
    SV = PVHV(0x2c160ac) at 0x375fe14
      REFCNT = 1
      FLAGS = (OBJECT,OOK,SHAREKEYS)
      STASH = 0x26ba574 "MainWindow"
      ARRAY = 0x37de13c  (0:7, 1:1)
      hash quality = 100.0%
      KEYS = 1
      FILL = 1
      MAX = 7
      RITER = -1
      EITER = 0x0
        Elt "_TkValue_" HASH = 0xf8f586ad
        SV = PV(0x370cfe4) at 0x375f774
          REFCNT = 1
          FLAGS = (POK,pPOK)
          PV = 0x37b5924 "."\0
          CUR = 1
          LEN = 4
  PV = 0x375fe14 ""
  CUR = 0
  LEN = 0
  MAGIC = 0x36dfdb4
    MG_VIRTUAL = 0x38104040
    MG_TYPE = PERL_MAGIC_ext(~)
    MG_FLAGS = 0x02
      REFCOUNTED
    MG_OBJ = 0x37601e4
        SV = PV(0x370d404) at 0x37601e4
          REFCNT = 1
          FLAGS = ()
          PV = 0x3797954 ""
          CUR = 0
          LEN = 20
Tk::Error: Usage $widget->destroy(...) at script/MICROSATELIGHT_v100_2.pl line 4
05.
 Tk callback for .frame.button1
 Tk::__ANON__ at Tk.pm line 250
 Tk::Button::butUp at Tk/Button.pm line 175
 <ButtonRelease-1>
 (command bound to event)



It is worth to note that I cannot access or modify the C:// drive (where the application is installed) as this computer is University property. I have been storing the output of PeakScanner on a different drive.



Any ideas?



Regards,



Juan



Juan F. Dueñas-Serrano
Post-Graduate Student (M. Appl. Sc.)
Faculty of Agricultural and Life Sciences
Burns 536
Lincoln University
New Zealand

[Microsatelight-updates] tetraploids?

[Bert O. <Ber...@mi...>]

Hi,
 
I came across this application while searching for microsatellite analysis tools.
The help file states that the genotype table contains 2 columns for each marker.
Could it be easily adapted towards 4 columns? In case of tetraploid species, more then 2 alleles are frequently observed per marker.
Is anything foreseen in the development pipeline for polyploids?
cheers,
Bert
 

_______________________________________________________
Maildisclaimer HTTP://www.flore.be/disclaimer.html