I'm trying to merge contigs from a mini-metagenome built with SPAdes and Metaspades asemblers using the step_by_step script. CE statisticts looks fine and metassemble MergePipeline worked outputting a fasta file. When the pipeline tried to retrieve sequences in the last step of Metassembler, the following error appear:
Error:
B.A_0 300901 447 0 NODE_3_length_300922_cov_62.4173 300901 447 + X X X X R.NODE_3_length_300922_cov_62.4173 212 447 Q.NODE_195_length_36249_cov_101.886 36249 36014
could not be retrieved
The test worked fine but I do not know what causes this error.
**Note: For simplicity I replaced the files "A" and "B" provided in the test with the contigs obtained from the assemblies.
Thanks in advance
Carlos
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Hi All
I'm trying to merge contigs from a mini-metagenome built with SPAdes and Metaspades asemblers using the step_by_step script. CE statisticts looks fine and metassemble MergePipeline worked outputting a fasta file. When the pipeline tried to retrieve sequences in the last step of Metassembler, the following error appear:
---------- Loading Fastas ----------
---Loading A.fa
---Loading B.fa
---------- Retrieving sequences ----------
Error:
B.A_0 300901 447 0 NODE_3_length_300922_cov_62.4173 300901 447 + X X X X R.NODE_3_length_300922_cov_62.4173 212 447 Q.NODE_195_length_36249_cov_101.886 36249 36014
could not be retrieved
The test worked fine but I do not know what causes this error.
**Note: For simplicity I replaced the files "A" and "B" provided in the test with the contigs obtained from the assemblies.
Thanks in advance
Carlos