Kenny Tsang - 2017-10-04

Dear all,

I have one illumina TruSeq long read assembly and three MiSeq 2x75 short reads assemblies. I want to merge the long read assembly to each of the short reads assemblies so that at the end I will have three total assmblies:

1) Long read scaffold + Velvet scaffolds
2) Long read scaffold + AbySS scaffolds
3) Long read scaffold + SOAP scaffolds

However, I do not know how to denife the parameters in the config file, it looks like this:

[global]

mateAn_A=200
mateAn_B=300
bowtie2_read1=/lustre/projects/holfordlab/ktsang/TSSLR-BDA11_LongRead.fastq
bowtie2_read2=/lustre/projects/holfordlab/ktsang/oenopla-reads1.fastq
bowtie2_maxins=500
bowtie2_minins=50
bowtie2_threads=1
meta2fasta_keepUnaligned=0
meta2fasta_sizeUnaligned=500 500
nucmer_l=50
nucmer_c=300

nucmer=/home/ktsang/.conda/envs/MyVirtualEnv/bin/nucmer
delta-filter=/home/ktsang/.conda/envs/MyVirtualEnv/bin/delta-filter
show-coords=/home/ktsang/.conda/envs/MyVirtualEnv/bin/show-coords
bowtie2=/home/ktsang/.conda/envs/MyVirtualEnv/bin/bowtie2
bowtie2-build=/home/ktsang/.conda/envs/MyVirtualEnv/bin/bowtie2-build
samtools=/home/ktsang/.conda/envs/MyVirtualEnv/bin/samtools

[1]
fasta=/lustre/projects/holfordlab/ktsang/oenopla_VOscaffolds011216.fasta
ID=LongRead

[2]
fasta=/lustre/projects/holfordlab/ktsang/scaffolds/SOAPdenovo.final.scaffolds.fasta
ID=SOAP

I have read the manual but I still do not understand. What number I should put at mateAn_A, mateAn_B, bowtie2_maxins, bowtie2_minins, meta2fasta?

I do not know the insert size of the short reads, can someone help me with deciding he numbers in bold?

Thank you!

Kenny