Is it true that only mate-pair reads, not paired end reads, can be used by metassembler? All I have is paired end data yet my assemblies are marked different and might benefit from merging.
Thanks
Mark
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You can run it with paired end data, just in our testing we dont see a lot
of improvement. The mate pairs (or paired-end reads) are used to decide
which assembly is more likely to be correct when the assemblies conflict
with each other. But you dont get enough resolution from paired end reads
since most conflicts are caused by repeats.
Is it true that only mate-pair reads, not paired end reads, can be used by
metassembler? All I have is paired end data yet my assemblies are marked
different and might benefit from merging.
Thanks
Mark
This message may contain confidential information. If you are not the
designated recipient, please notify the sender immediately, and delete the
original and any copies. Any use of the message by you is prohibited.
Hello
Is it true that only mate-pair reads, not paired end reads, can be used by metassembler? All I have is paired end data yet my assemblies are marked different and might benefit from merging.
Thanks
Mark
This message may contain confidential information. If you are not the designated recipient, please notify the sender immediately, and delete the original and any copies. Any use of the message by you is prohibited.
You can run it with paired end data, just in our testing we dont see a lot
of improvement. The mate pairs (or paired-end reads) are used to decide
which assembly is more likely to be correct when the assemblies conflict
with each other. But you dont get enough resolution from paired end reads
since most conflicts are caused by repeats.
Good luck
Mike
On Sat, Jan 21, 2017 at 2:28 PM, Mark rosema1@users.sf.net wrote: