#12 Error: failed to get abundance information

[Scott Klasek]

Hi, I'm trying to run maxbin2 using the command below
run_MaxBin.pl -contig /usr/local/tmp/tmpuaykixwj/sequence_contigs.fa -abund /usr/local/tmp/tmpuaykixwj/contig_coverages.txt -out /usr/local/tmp/tmpuaykixwj/MAXBIN_ -thread 36

and I encountered this error:

Failed to get abudance information from input file [/usr/local/tmp/tmpuaykixwj/MAXBIN_.contig.tmp.abund1].
The error lies in the following line:
===
0825536598789213
===
Please check your input abundance files.
Error encountered while running core MaxBin program. Error recorded in /usr/local/tmp/tmpuaykixwj/MAXBIN_.log.
Program Stop.

I do not have anywhere close to 825536598789213 lines in my file-- it's "only" 2.3 million lines and 3 Gb-- but the number 0.0825536598789213 appears in several lines. I've successfully run maxbin2 on a smaller dataset so I don't think it's an environment issue. (In case it's relevant, I'm running maxbin2 from the anvi-cluster-contigs command in anvio and running CentOS 7.9).
Any suggestions or even just an interpretation of the error?
Thanks,
Scott

[Shankha Nath]

I also have encountered exactly same error for Maxbin2.. Only in my case the line number is 5739..

please help ..

nohup: ignoring input
MaxBin 2.2.7
Input contig: /data/sata_data/home/souvik_p1/sewage_analysis/analysis/trimmomatic_out/CLEAN_READS/COASSEMBLY_OUTPUT/assembly.fa
Located abundance file [/data/sata_data/home/souvik_p1/sewage_analysis/analysis/trimmomatic_out/CLEAN_READS/Metawrap_megahit/maxbin2_ready_depth.txt]
out header: /data/sata_data/home/souvik_p1/sewage_analysis/analysis/trimmomatic_out/CLEAN_READS/Metawrap_megahit/INITIAL_BINNING/maxbin2_bins/
Min contig length: 1500
Thread: 80
Searching against 107 marker genes to find starting seed contigs for [/data/sata_data/home/souvik_p1/sewage_analysis/analysis/trimmomatic_out/CLEAN_READS/COASSEMBLY_OUTPUT/assembly.fa]...
Running FragGeneScan....
Running HMMER hmmsearch....
Done data collection. Running MaxBin...
Command: /data/sata_data/home/souvik_p1/miniconda3/envs/maxbin2-env/opt/MaxBin-2.2.7/src/MaxBin -fasta /data/sata_data/home/souvik_p1/sewage_analysis/analysis/trimmomatic_out/CLEAN_READS/Metawrap_megahit/INITIAL_BINNING/maxbin2_bins/.contig.tmp  -abund /data/sata_data/home/souvik_p1/sewage_analysis/analysis/trimmomatic_out/CLEAN_READS/Metawrap_megahit/INITIAL_BINNING/maxbin2_bins/.contig.tmp.abund1 -seed /data/sata_data/home/souvik_p1/sewage_analysis/analysis/trimmomatic_out/CLEAN_READS/Metawrap_megahit/INITIAL_BINNING/maxbin2_bins/.seed -out /data/sata_data/home/souvik_p1/sewage_analysis/analysis/trimmomatic_out/CLEAN_READS/Metawrap_megahit/INITIAL_BINNING/maxbin2_bins/ -min_contig_length 1500 -verbose -thread 80
Loaded 100000 sequences
Loaded 200000 sequences
Loaded 300000 sequences
Loaded 400000 sequences
Loaded 500000 sequences
Loaded 600000 sequences
Loaded 700000 sequences
Loaded 800000 sequences
Loaded 900000 sequences
Loaded 1000000 sequences
Loaded 1100000 sequences
Loaded 1200000 sequences
Loaded 1300000 sequences
Loaded 1400000 sequences
Loaded 1500000 sequences
Loaded 1600000 sequences
Loaded 1700000 sequences
Loaded 1800000 sequences
Loaded 1900000 sequences
Loaded 2000000 sequences
Loaded 2100000 sequences
Loaded 2200000 sequences
Loaded 2300000 sequences
Loaded 2400000 sequences
Loaded 2500000 sequences
Loaded 2600000 sequences
Loaded 2700000 sequences
Loaded 2800000 sequences
Loaded 2900000 sequences
Loaded 3000000 sequences
Loaded 3100000 sequences
Loaded 3200000 sequences
Loaded 3300000 sequences
Loaded 3400000 sequences
Loaded 3500000 sequences
Loaded 3600000 sequences
Loaded 3700000 sequences
Loaded 3800000 sequences
Failed to get abudance information from input file [/data/sata_data/home/souvik_p1/sewage_analysis/analysis/trimmomatic_out/CLEAN_READS/Metawrap_megahit/INITIAL_BINNING/maxbin2_bins/.contig.tmp.abund1].
The error lies in the following line:
===
5739
===
Please check your input abundance files.
Error encountered while running core MaxBin program. Error recorded in /data/sata_data/home/souvik_p1/sewage_analysis/analysis/trimmomatic_out/CLEAN_READS/Metawrap_megahit/INITIAL_BINNING/maxbin2_bins/.log.
Program Stop.