#5 MaxBin 2.2.7 failed on CentOS7

[Igor Molchanov]

Hi, I had an issue while trying to run MaxBin 2.2.7 on CentOS7.

The error message was the following: "maxbin/2.2.7/app/maxbin/auxiliary/hmmer-3.1b1/src/hmmsearch: /lib64/libm.so.6: version 'GLIBC_2.27' not found (required by maxbin/2.2.7/app/maxbin/auxiliary/hmmer-3.1b1/src/hmmsearch)".

CentOS7 only has v2.17 available.
Is it possible to solve this issue and run MaxBin on CentOS7 ?

[Yu-Wei Wu]

I tried to test MaxBin on a clean centOS system (simulated using docker centos:latest) and identified that MaxBin can run on centOS. Here are the things that I do to make MaxBin running on the very clean system.

1. sudo yum groupinstall 'Development Tools'
2. sudo yum install perl-CPAN
3. sudo yum install which

In the above steps, step 1 installs most necessary packages for compiling programs; step 2 installs CPAN for PERL, and step 3 installs which packages. The three steps may not be neede in every centOS system; I just found that the three are required for the docker-simulated centOS.

There is also one thing: I need to add a few lines in the "autobuild_auxiliary" script for automatically downloading and installing other software. After adding a few lines I was able to

1. run "autobuild_auxiliary" script to automatically download and install software in the MaxBin folder
2. enter "src" folder to "make" the program
3. try MaxBin on a test dataset (such as 20X dataset available on https://downloads.jbei.org/data/microbial_communities/MaxBin/MaxBin.html)

The updated autobuild_auxiliary script is available in the attachment. I have not updated the software version with this script since I have not extensively tested the script everywhere. Please see if this script and the aforementioned requirements/steps work for you.

[Igor Molchanov]

I have checked it and it all works now, thank you. Can you update the software now to make it possible to use this script in MaxBin ?