Hi, I'm trying to order some PacBio contigs (attached file: META_ancMR-1_PacBio.fasta) by aligning them with a reference genome (attached file: MR1_NCBI_genomic.fasta). When I try to do this by choosing Tools>Move Contigs and then specifying my output folder and input sequences (attached), I get the following readout from the Mauve Console:
"Running alignment.
Executing
win64\progressiveMauve
--output=F:\Mauve_output\alignment1\alignment1
--skip-refinement
--weight=200
--output-guide-tree=F:\Mauve_output\alignment1\alignment1.guide_tree
--backbone-output=F:\Mauve_output\alignment1\alignment1.backbone
F:\Mauve_output\MR1_NCBI_genomic.fasta\MR1_NCBI_genomic.fasta
F:\Mauve_output\META_ancMR-1_PacBio.fasta\META_ancMR-1_PacBio.fasta
Exception FileNotOpened thrown from
Unknown() in ..\libGenome\gnFileSource.cpp 67
Called by Unknown()
Exited with error code: -1073741676"
If there is a way to resolve this, I would greatly appreciate any help. Thanks!