Re: [maq-help] (no subject)

[Noll, A. <ac...@St...>]

Jennifer,

This is the solexa fastq format.

Take a look at http://seqanswers.com/forums/showthread.php?t=468

Hope that helps,

Aaron Noll
Stowers Institute for Medical Research
Kansas City, MO
________________________________________
From: maq...@li... [maq...@li...] On Behalf Of jennifer mackinnon [jen...@ma...]
Sent: Wednesday, August 13, 2008 10:53 PM
To: maq...@li...
Subject: [maq-help] (no subject)

Hello,

I am trying to use maq.pl on a mac.  Can you kindly identify if the following is solexa fastq or standard fastq:

@HWI-EAS153_2_206A5AAXX:2:1:919:287
GAATGCCGTCTTCTGCTTGAAAAAGCGAACATATGC
+HWI-EAS153_2_206A5AAXX:2:1:919:287
hhhhhZhMNVf]GF?NhPLA=EEQCHC?D=<?@B<H
@HWI-EAS153_2_206A5AAXX:2:1:934:913
GCATTTAAAGACATCCACTCGAAGTCCGCATTCTCT
+HWI-EAS153_2_206A5AAXX:2:1:934:913
hQPhTh^@BMT@D@BLE>BB=@?=@IAH=A?=>???
@HWI-EAS153_2_206A5AAXX:2:1:914:185
GAGACTTCAAAATACTTAAAAAGATCGGAAGAGCTC
+HWI-EAS153_2_206A5AAXX:2:1:914:185
hhENGhK[aDVHQX[PJDECHEEIDGFD@AGAEBBB

It seems that the file type is not recognized when I run maq.pl easyrun or fastq2bfq

Thank you!