LpGsAoH

This analytical program LpGsAoH is designed for identification of absence of heterozygosity with low-pass (low-coverage) genome sequencing data. Based on our evaluation, the minimal read-depth for AOH identification is 4-fold. In addition, as the program is designed for low-pass GS, the maximum read-depth is 10-fold. If the read-depth of your data exceed the cutoff, please perform a randomly selection of read/read-pairs to generate a “low-pass” GS for your sample. Pls be noted that this method can be applied regardless of sequencing platforms and library construction methods. Most importantly, it is built up based on the human genome reference of GRCh38/hg38.