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ZLimit in lepmap2 and filtering

Homa
2016-08-09
2016-08-10
  • Homa

    Homa - 2016-08-09

    Hello,

    Thank you for the great software.

    I am working with the sex chromosome linkage map. In order to take into account sex linked markers, I used ZLimit=2. When I compare the genotypes before and after, I see the following change:
    Genotypes before ParentCall:
    father 1 2
    mother 2 2
    daughter 1 1

    After using ZLimit=2, the daughter genotype has turned into 1 2. I was wondering how in later stages, these cases are recognized as sex linked and are not treated as an autosomal marker?

    Thank you very much,
    Best,
    Homa

     

    Last edit: Homa 2016-08-09
  • Pasi Rastas

    Pasi Rastas - 2016-08-10

    Hello Homa,

    Thank you for your email.

    X/ZLimit will convert sex inherited markers into autosomal like markers. There will be star in the output of ParentCall indicating the marker is converted.

    Without ZXLimit, there would be Mendel errors in the data. Then either the marker would not be called or the parental or children's genotypes would be changed (in your case the mother to 1 2).

    Cheers,
    Pasi

     

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