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Segmentation fault while running RNASEQ sample alone

2016-02-08
2016-02-09
  • Naresh Prodduturi

    I'm getting segmentation fault error when i'm trying to run Integrate software on mRNASeq samples (In the past i ran it successfully on other samples)

    /data2/labdev/mgf/dev/naresh/MGF/RNASEQ_DELETION/INDEL_SOMATICSNV/Workflow_data/INTEGRATE_0_2_0/Integrate/INTEGRATE-build/bin/Integrate fusion /data2/labdev/mgf/dev/references/hg19/37.1_allchr.fa /data2/labdev/mgf/dev/naresh/MGF/RNASEQ_DELETION/INDEL_SOMATICSNV/Workflow_data/INTEGRATE_0_2_0/Integrate/INTEGRATE-build/bin/annot.ucsc.txt /data2/labdev/mgf/dev/zxs_analysis/lungad/rnaseq_5samp_10_22_2015/output2/ALIGNMENT/STAR1/bwts ../SRR950078.GSNAP.gatkin.bam ../SRR950078.GSNAP.gatkin.bam
    INTEGRATE version 0.2.0
    Loading reference...
    chr1 1 249250621
    chr2 249250622 492449994
    chr3 492449995 690472424
    chr4 690472425 881626700
    chr5 881626701 1062541960
    chr6 1062541961 1233657027
    chr7 1233657028 1392795690
    chr8 1392795691 1539159712
    chr9 1539159713 1680373143
    chr10 1680373144 1815907890
    chr11 1815907891 1950914406
    chr12 1950914407 2084766301
    chr13 2084766302 2199936179
    chr14 2199936180 2307285719
    chr15 2307285720 2409817111
    chr16 2409817112 2500171864
    chr17 2500171865 2581367074
    chr18 2581367075 2659444322
    chr19 2659444323 2718573305
    chr20 2718573306 2781598825
    chr21 2781598826 2829728720
    chr22 2829728721 2881033286
    chrX 2881033287 3036303846
    chrY 3036303847 3095677412
    chrM 3095677413 3095693981
    2.75 seconds

    Loading genes...
    78827 transcripts loaded.
    0.56 seconds

    Loading BWTs for chromosomes...
    51.92 seconds

    Handling RNA data...

    Insert sizes and stds tested by different RGs:
    group1 0 985

    Getting graph by encompassing RNA reads...
    Segmentation fault

    please give me some suggestions.

    Regards
    Naresh

     
    • Jin

      Jin - 2016-02-08

      Are the BAMs for other successful finished samples prepared the same way as the mRNAseq samples?

      All mRNAseq samples failed or just one?

       
      • Naresh Prodduturi

        Yes they were prepared the same way (aligner GSNAP).
        All new samples got failed.

         

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