htseq counting problem
Analysing high-throughput sequencing data with Python
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Hi all
I tried to use htseq-count to count reads number from RNA-Seq. There are two groups, control and treatment. Each group has 3 replicates. After this command - noint <- rownames(counts) %in% c("no_feature","ambiguous","too_low_aQual","not_aligned","alignment_not_unique")- I got noint. But there are only five features which are TRUE. Anyone would help me with that?
Sorry, I can't follow your question. It sounds as if you are following some instructions or tutorial. Which one? And what have you done before this command?