From: Steve F. <sfi...@pc...> - 2007-05-18 16:16:34
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alberto- the code is trying to insert a database cross reference to HSSP. to do this it must create rows in ExternalDatabase and ExternaDatabaseRelease. the problem is that when it tries to insert a row into ExternalDatabase, it is using a primary key (18) that is already in use in that table. the first guesses are that: - there is something wrong with the database mechanism (postgres) to generate sequential primary keys - somebody manually inserted a row into that table, using 18 as a primary key, without incrementing the primary key generating mechanism steve Alberto Davila wrote: > Hi folks ! > > We managed to pass several errors with ISF but this one is a bit strange: > > [davila1@kineto4 log]$ ga > GUS::Supported::Plugin::InsertSequenceFeatures --mapFile > /system/GUS/gus_home/config/genbank2gus.xml --inputFileOrDir > /home/davila1/lguyanensis.gb --fileFormat genbank --extDbName GenBank > --extDbRlsVer 159.0 --organism "Leishmania guyanensis" > --defaultOrganism "Leishmania guyanensis" --verbose --soCvsVersion 1.45 > Fri May 18 12:42:57 2007 DSN dbi:Pg:dbname=protozoa > Fri May 18 12:42:57 2007 PLUGIN > GUS::Supported::Plugin::InsertSequenceFeatures > Fri May 18 12:42:57 2007 ARG algoinvo 1 > Fri May 18 12:42:57 2007 ARG bioperlTreeOutput 0 > Fri May 18 12:42:57 2007 ARG comment > Fri May 18 12:42:57 2007 ARG commit 0 > Fri May 18 12:42:57 2007 ARG debug 0 > Fri May 18 12:42:57 2007 ARG defaultOrganism Leishmania > guyanensis > Fri May 18 12:42:57 2007 ARG extDbName GenBank > Fri May 18 12:42:57 2007 ARG extDbRlsVer 159.0 > Fri May 18 12:42:57 2007 ARG fileFormat genbank > Fri May 18 12:42:57 2007 ARG gff2GroupTag > Fri May 18 12:42:57 2007 ARG group > Fri May 18 12:42:57 2007 ARG gusconfigfile > /system/GUS/gus_home/config/gus.config > Fri May 18 12:42:57 2007 ARG handlerExternalDbs > Fri May 18 12:42:57 2007 ARG help > Fri May 18 12:42:57 2007 ARG helpHTML > Fri May 18 12:42:57 2007 ARG inputFileExtension > Fri May 18 12:42:57 2007 ARG inputFileOrDir > /home/davila1/lguyanensis.gb > Fri May 18 12:42:57 2007 ARG mapFile > /system/GUS/gus_home/config/genbank2gus.xml > Fri May 18 12:42:57 2007 ARG naSequenceSubclass > Fri May 18 12:42:57 2007 ARG organism Leishmania > guyanensis > Fri May 18 12:42:57 2007 ARG project > Fri May 18 12:42:57 2007 ARG seqExtDbName > Fri May 18 12:42:57 2007 ARG seqExtDbRlsVer > Fri May 18 12:42:57 2007 ARG seqIdColumn source_id > Fri May 18 12:42:57 2007 ARG seqSoTerm > Fri May 18 12:42:57 2007 ARG seqType > Fri May 18 12:42:57 2007 ARG soCvsVersion 1.45 > Fri May 18 12:42:57 2007 ARG sqlVerbose 0 > Fri May 18 12:42:57 2007 ARG testNumber > Fri May 18 12:42:57 2007 ARG user > Fri May 18 12:42:57 2007 ARG verbose 1 > Fri May 18 12:42:57 2007 ARG veryVerbose 0 > Fri May 18 12:42:57 2007 AlgInvocationId 83 > Fri May 18 12:42:57 2007 COMMIT commit off > Fri May 18 12:42:58 2007 Processing file > '/home/davila1/lguyanensis.gb'... > DBD::Pg::st execute failed: ERROR: duplicate key violates unique > constraint "pk_externaldatabase" > > ERROR: > > SQL ERROR!! involving > > INSERT INTO SRes.ExternalDatabase ( group_write, other_read, > group_read, name, user_read, row_user_id, external_database_id, > modification_date, other_write, row_alg_invocation_id, row_project_id, > row_group_id, user_write ) > VALUES ( ?, ?, ?, ?, ?, ?, ?, now(), ?, ?, ?, ?, ? ) > Values: 1, 1, 1, HSSP, 1, 1, 18, 0, 83, 1, 1, 1 at > /system/GUS/gus_home/lib/perl/GUS/ObjRelP/DbiDbHandle.pm line 166 > > GUS::ObjRelP::DbiDbHandle::death('GUS::ObjRelP::DbiDbHandle=HASH(0x1e619d0)', > '\x{a} SQL ERROR!! involving\x{a} \x{a} INSERT INTO > SRes.ExternalDataba...') called at > /system/GUS/gus_home/lib/perl/GUS/ObjRelP/DbiDbHandle.pm line 150 > > GUS::ObjRelP::DbiDbHandle::sqlExec('GUS::ObjRelP::DbiDbHandle=HASH(0x1e619d0)', > 'GUS::ObjRelP::DbiDbHandle::st=HASH(0x30dae70)', 'ARRAY(0x30daec0)', > '\x{a} INSERT INTO SRes.ExternalDatabase ( group_write, > other_...') called at > /system/GUS/gus_home/lib/perl/GUS/ObjRelP/DbiRow.pm line 673 > > GUS::ObjRelP::DbiRow::quote_and_insert('GUS::Model::SRes::ExternalDatabase=HASH(0x30d4b00)', > 'HASH(0x30d4870)') called at > /system/GUS/gus_home/lib/perl/GUS/ObjRelP/DbiRow.pm line 620 > > GUS::ObjRelP::DbiRow::insert('GUS::Model::SRes::ExternalDatabase=HASH(0x30d4b00)') > called at /system/GUS/gus_home/lib/perl/GUS/Model/GusRow.pm line 1692 > > GUS::Model::GusRow::submit('GUS::Model::SRes::ExternalDatabase=HASH(0x30d4b00)') > called at > /system/GUS/gus_home/lib/perl/GUS/Supported/SpecialCaseQualifierHandlers.pm > line 160 > > GUS::Supported::SpecialCaseQualifierHandlers::_getExtDatabaseRlsId('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdda20)', > 'HSSP') called at > /system/GUS/gus_home/lib/perl/GUS/Supported/SpecialCaseQualifierHandlers.pm > line 134 > > GUS::Supported::SpecialCaseQualifierHandlers::_getDbXRefId('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdda20)', > 'HSSP:P07711') called at > /system/GUS/gus_home/lib/perl/GUS/Supported/SpecialCaseQualifierHandlers.pm > line 117 > > GUS::Supported::SpecialCaseQualifierHandlers::buildDbXRef('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdda20)', > 'HSSP:P07711') called at > /system/GUS/gus_home/lib/perl/GUS/Supported/SpecialCaseQualifierHandlers.pm > line 106 > > GUS::Supported::SpecialCaseQualifierHandlers::dbXRef('GUS::Supported::SpecialCaseQualifierHandlers=HASH(0x10694f0)', > 'db_xref', 'Bio::SeqFeature::Generic=HASH(0x2d2e390)', > 'GUS::Model::DoTS::Transcript=HASH(0x2f3bc00)') called at > /system/GUS/gus_home/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm > line 1021 > > GUS::Supported::Plugin::InsertSequenceFeatures::handleFeatureTag('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdda20)', > 'Bio::SeqFeature::Generic=HASH(0x2d2e390)', > 'GUS::Supported::BioperlFeatMapper=HASH(0x2456250)', > 'GUS::Model::DoTS::Transcript=HASH(0x2f3bc00)', 'db_xref') called at > /system/GUS/gus_home/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm > line 891 > > GUS::Supported::Plugin::InsertSequenceFeatures::applyQualifiers('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdda20)', > 'Bio::SeqFeature::Generic=HASH(0x2d2e390)') called at > /system/GUS/gus_home/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm > line 897 > > GUS::Supported::Plugin::InsertSequenceFeatures::applyQualifiers('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdda20)', > 'Bio::SeqFeature::Generic=HASH(0x30cdbe0)') called at > /system/GUS/gus_home/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm > line 897 > > GUS::Supported::Plugin::InsertSequenceFeatures::applyQualifiers('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdda20)', > 'Bio::SeqFeature::Generic=HASH(0x3081dd0)') called at > /system/GUS/gus_home/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm > line 771 > > GUS::Supported::Plugin::InsertSequenceFeatures::processFeatureTrees('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdda20)', > 'Bio::Seq::RichSeq=HASH(0x300b780)', 96, 171, 'undef') called at > /system/GUS/gus_home/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm > line 367 > > GUS::Supported::Plugin::InsertSequenceFeatures::run('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdda20)', > 'HASH(0x2062a70)') called at > /system/GUS/gus_home/lib/perl/GUS/PluginMgr/GusApplication.pm line 580 > eval {...} called at > /system/GUS/gus_home/lib/perl/GUS/PluginMgr/GusApplication.pm line 569 > > GUS::PluginMgr::GusApplication::doMajorMode_RunOrReport('GUS::PluginMgr::GusApplication=HASH(0x5052a0)', > 'GUS::Supported::Plugin::InsertSequenceFeatures', 1) called at > /system/GUS/gus_home/lib/perl/GUS/PluginMgr/GusApplication.pm line 485 > > GUS::PluginMgr::GusApplication::doMajorMode_Run('GUS::PluginMgr::GusApplication=HASH(0x5052a0)', > 'GUS::Supported::Plugin::InsertSequenceFeatures') called at > /system/GUS/gus_home/lib/perl/GUS/PluginMgr/GusApplication.pm line 383 > > GUS::PluginMgr::GusApplication::doMajorMode('GUS::PluginMgr::GusApplication=HASH(0x5052a0)', > 'GUS::Supported::Plugin::InsertSequenceFeatures') called at > /system/GUS/gus_home/lib/perl/GUS/PluginMgr/GusApplication.pm line 292 > > GUS::PluginMgr::GusApplication::parseAndRun('GUS::PluginMgr::GusApplication=HASH(0x5052a0)', > 'ARRAY(0x505dd0)') called at /system/GUS/gus_home/bin/ga line 11 > > STACK TRACE: > at /system/GUS/gus_home/lib/perl/GUS/ObjRelP/DbiDbHandle.pm line 166 > > GUS::ObjRelP::DbiDbHandle::death('GUS::ObjRelP::DbiDbHandle=HASH(0x1e619d0)', > '\x{a} SQL ERROR!! involving\x{a} \x{a} INSERT INTO > SRes.ExternalDataba...') called at > /system/GUS/gus_home/lib/perl/GUS/ObjRelP/DbiDbHandle.pm line 150 > > GUS::ObjRelP::DbiDbHandle::sqlExec('GUS::ObjRelP::DbiDbHandle=HASH(0x1e619d0)', > 'GUS::ObjRelP::DbiDbHandle::st=HASH(0x30dae70)', 'ARRAY(0x30daec0)', > '\x{a} INSERT INTO SRes.ExternalDatabase ( group_write, > other_...') called at > /system/GUS/gus_home/lib/perl/GUS/ObjRelP/DbiRow.pm line 673 > > GUS::ObjRelP::DbiRow::quote_and_insert('GUS::Model::SRes::ExternalDatabase=HASH(0x30d4b00)', > 'HASH(0x30d4870)') called at > /system/GUS/gus_home/lib/perl/GUS/ObjRelP/DbiRow.pm line 620 > > GUS::ObjRelP::DbiRow::insert('GUS::Model::SRes::ExternalDatabase=HASH(0x30d4b00)') > called at /system/GUS/gus_home/lib/perl/GUS/Model/GusRow.pm line 1692 > > GUS::Model::GusRow::submit('GUS::Model::SRes::ExternalDatabase=HASH(0x30d4b00)') > called at > /system/GUS/gus_home/lib/perl/GUS/Supported/SpecialCaseQualifierHandlers.pm > line 160 > > GUS::Supported::SpecialCaseQualifierHandlers::_getExtDatabaseRlsId('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdda20)', > 'HSSP') called at > /system/GUS/gus_home/lib/perl/GUS/Supported/SpecialCaseQualifierHandlers.pm > line 134 > > GUS::Supported::SpecialCaseQualifierHandlers::_getDbXRefId('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdda20)', > 'HSSP:P07711') called at > /system/GUS/gus_home/lib/perl/GUS/Supported/SpecialCaseQualifierHandlers.pm > line 117 > > GUS::Supported::SpecialCaseQualifierHandlers::buildDbXRef('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdda20)', > 'HSSP:P07711') called at > /system/GUS/gus_home/lib/perl/GUS/Supported/SpecialCaseQualifierHandlers.pm > line 106 > > GUS::Supported::SpecialCaseQualifierHandlers::dbXRef('GUS::Supported::SpecialCaseQualifierHandlers=HASH(0x10694f0)', > 'db_xref', 'Bio::SeqFeature::Generic=HASH(0x2d2e390)', > 'GUS::Model::DoTS::Transcript=HASH(0x2f3bc00)') called at > /system/GUS/gus_home/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm > line 1021 > > GUS::Supported::Plugin::InsertSequenceFeatures::handleFeatureTag('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdda20)', > 'Bio::SeqFeature::Generic=HASH(0x2d2e390)', > 'GUS::Supported::BioperlFeatMapper=HASH(0x2456250)', > 'GUS::Model::DoTS::Transcript=HASH(0x2f3bc00)', 'db_xref') called at > /system/GUS/gus_home/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm > line 891 > > GUS::Supported::Plugin::InsertSequenceFeatures::applyQualifiers('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdda20)', > 'Bio::SeqFeature::Generic=HASH(0x2d2e390)') called at > /system/GUS/gus_home/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm > line 897 > > GUS::Supported::Plugin::InsertSequenceFeatures::applyQualifiers('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdda20)', > 'Bio::SeqFeature::Generic=HASH(0x30cdbe0)') called at > /system/GUS/gus_home/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm > line 897 > > GUS::Supported::Plugin::InsertSequenceFeatures::applyQualifiers('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdda20)', > 'Bio::SeqFeature::Generic=HASH(0x3081dd0)') called at > /system/GUS/gus_home/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm > line 771 > > GUS::Supported::Plugin::InsertSequenceFeatures::processFeatureTrees('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdda20)', > 'Bio::Seq::RichSeq=HASH(0x300b780)', 96, 171, 'undef') called at > /system/GUS/gus_home/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm > line 367 > > GUS::Supported::Plugin::InsertSequenceFeatures::run('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdda20)', > 'HASH(0x2062a70)') called at > /system/GUS/gus_home/lib/perl/GUS/PluginMgr/GusApplication.pm line 580 > eval {...} called at > /system/GUS/gus_home/lib/perl/GUS/PluginMgr/GusApplication.pm line 569 > > GUS::PluginMgr::GusApplication::doMajorMode_RunOrReport('GUS::PluginMgr::GusApplication=HASH(0x5052a0)', > 'GUS::Supported::Plugin::InsertSequenceFeatures', 1) called at > /system/GUS/gus_home/lib/perl/GUS/PluginMgr/GusApplication.pm line 485 > > GUS::PluginMgr::GusApplication::doMajorMode_Run('GUS::PluginMgr::GusApplication=HASH(0x5052a0)', > 'GUS::Supported::Plugin::InsertSequenceFeatures') called at > /system/GUS/gus_home/lib/perl/GUS/PluginMgr/GusApplication.pm line 383 > > GUS::PluginMgr::GusApplication::doMajorMode('GUS::PluginMgr::GusApplication=HASH(0x5052a0)', > 'GUS::Supported::Plugin::InsertSequenceFeatures') called at > /system/GUS/gus_home/lib/perl/GUS/PluginMgr/GusApplication.pm line 292 > > GUS::PluginMgr::GusApplication::parseAndRun('GUS::PluginMgr::GusApplication=HASH(0x5052a0)', > 'ARRAY(0x505dd0)') called at /system/GUS/gus_home/bin/ga line 11 > > This is my external_database table: > > -- PostgreSQL database dump > -- > > SET client_encoding = 'UTF8'; > SET standard_conforming_strings = off; > SET check_function_bodies = false; > SET client_min_messages = warning; > SET escape_string_warning = off; > > SET search_path = sres, pg_catalog; > > > COPY externaldatabase (external_database_id, name, modification_date, > user_read, user_write, group_read, group_write, other_read, > other_write, row_user_id, row_group_id, row_project_id, > row_alg_invocation_id) FROM stdin; > 164 RepBase 4.03 zebrep.ref 4.0.0 2007-05-06 22:04:51.888548 > 1 1 1 1 1 0 1 1 1 3 > 106 maize-2dpage 2007-05-06 22:04:51.888548 1 1 1 > 1 1 0 1 1 1 3 > 2093 homstrad 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 89 mim 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 1 assembly 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 154 RepBase 4.03 celrep.ref 4.0.4 2007-05-06 22:04:51.888548 > 1 1 1 1 1 0 1 1 1 3 > 82 mendel 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 185 RIKEN/FANTOM 2007-05-06 22:04:51.888548 1 1 1 > 1 1 0 1 1 1 3 > 292 Plasmodium_GSS 2007-05-06 22:04:51.888548 1 1 1 > 1 1 0 1 1 1 3 > 102 hsc-2dpage 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 2096 PRODOM 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 189 COMPEL 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 3010 GeneDB_Lmajor 2007-05-06 22:04:51.888548 1 1 1 > 1 1 0 1 1 1 3 > 2095 load 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 97 subtilist 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 90 mgd 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 17 GSDB 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 92 aarhus/ghent-2dpage 2007-05-06 22:04:51.888548 1 1 > 1 1 1 0 1 1 1 3 > 8 Brookhaven Protein Data Bank (nrdb) 2007-05-06 > 22:04:51.888548 1 1 1 1 1 0 1 1 1 3 > 184 ORNL GRAILExp gene predictions 2007-05-06 22:04:51.888548 > 1 1 1 1 1 0 1 1 1 3 > 12 GenPept 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 692 Plasmodium_TIGR 2007-05-06 22:04:51.888548 1 1 1 > 1 1 0 1 1 1 3 > 144 flybase translated orfs 2007-05-06 22:04:51.888548 1 > 1 1 1 1 0 1 1 1 3 > 3008 GeneDB_Afumigatus 2007-05-06 22:04:51.888548 1 1 > 1 1 1 0 1 1 1 3 > 165 RepBase 4.03 humsub.ref 1.0.2 2007-05-06 22:04:51.888548 > 1 1 1 1 1 0 1 1 1 3 > 13 IMAGE 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 94 ecogene 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 3024 GOA 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 3011 GeneDB_Pberghei 2007-05-06 22:04:51.888548 1 1 > 1 1 1 0 1 1 1 3 > 3018 GeneDB_Bpertussis 2007-05-06 22:04:51.888548 1 1 > 1 1 1 0 1 1 1 3 > 1392 CDD 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 3 EMBL Data Library (nrdb) 2007-05-06 22:04:51.888548 1 > 1 1 1 1 0 1 1 1 3 > 2097 PFAMA 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 172 URL 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 108 hiv 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 101 stygene 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 3014 GeneDB_Tannulata 2007-05-06 22:04:51.888548 1 1 > 1 1 1 0 1 1 1 3 > 181 Signaling PAthway Database (SPAD) 2007-05-06 > 22:04:51.888548 1 1 1 1 1 0 1 1 1 3 > 10 GenInfo Backbone Id (nrdb) 2007-05-06 22:04:51.888548 1 > 1 1 1 1 0 1 1 1 3 > 2092 dbSNP 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 78 embl 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 84 prosite 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 3025 PubMed 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 6 Protein Research Foundation (nrdb) 2007-05-06 > 22:04:51.888548 1 1 1 1 1 0 1 1 1 3 > 928 GI 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 85 flybase 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 14 EGAD 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 126 MEDLINE 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 1792 dbSTS 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 156 RepBase 4.03 humrep.ref 7.0.6 2007-05-06 22:04:51.888548 > 1 1 1 1 1 0 1 1 1 3 > 152 TUBERCULIST 2007-05-06 22:04:51.888548 1 1 1 > 1 1 0 1 1 1 3 > 3005 GOC 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 392 EPCon 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 1892 PID 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 98 eco2dbase 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 104 transfac 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 293 Plasmodium_EST 2007-05-06 22:04:51.888548 1 1 1 > 1 1 0 1 1 1 3 > 127 sptrembl 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 130 imgt/ligm 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 1292 RATMAP 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 1192 ATCC 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 3001 GO Function 2007-05-06 22:04:51.888548 1 1 1 > 1 1 0 1 1 1 3 > 124 SWISS-PROT 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 1494 CDD-Pfam 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 143 NCBI genomic Homo Sapiens 2007-05-06 22:04:51.888548 1 > 1 1 1 1 0 1 1 1 3 > 18 DOTS 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 133 estlib 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 161 RepBase 4.03 rodrep.ref 3.3.0 2007-05-06 22:04:51.888548 > 1 1 1 1 1 0 1 1 1 3 > 157 RepBase 4.03 invrep.ref 4.0.0 2007-05-06 22:04:51.888548 > 1 1 1 1 1 0 1 1 1 3 > 1495 CDD-Smart 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 79 pfam 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 103 carbbank 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 191 Whitehead Institute/MIT Center for Genome Research Mouse RH > Map 2007-05-06 22:04:51.888548 1 1 1 1 1 0 1 > 1 1 3 > 155 RepBase 4.03 drorep.ref 4.0.0 2007-05-06 22:04:51.888548 > 1 1 1 1 1 0 1 1 1 3 > 125 PSEUDO 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 3006 GeneDB_Spombe 2007-05-06 22:04:51.888548 1 1 1 > 1 1 0 1 1 1 3 > 3022 PMID 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 153 RepBase 4.03 athrep.ref 4.0.5 2007-05-06 22:04:51.888548 > 1 1 1 1 1 0 1 1 1 3 > 122 SPTREMBL 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 186 UCSC human draft genomic sequence (October 7 release) > 2007-05-06 22:04:51.888548 1 1 1 1 1 0 1 1 > 1 3 > 179 GeneMap 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 135 agis 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 3013 GeneDB_Pfalciparum 2007-05-06 22:04:51.888548 1 1 > 1 1 1 0 1 1 1 3 > 170 Pfam expert 2007-05-06 22:04:51.888548 1 1 1 > 1 1 0 1 1 1 3 > 178 StemCellDB public 2007-05-06 22:04:51.888548 1 1 > 1 1 1 0 1 1 1 3 > 149 Bucan lab. mouse BAC ends 2007-05-06 22:04:51.888548 1 > 1 1 1 1 0 1 1 1 3 > 148 Prodom 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 3012 GeneDB_Pchabaudi 2007-05-06 22:04:51.888548 1 1 > 1 1 1 0 1 1 1 3 > 128 pseudo 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 175 INTERPRO 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 80 MEDLINE 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 3002 GO Component 2007-05-06 22:04:51.888548 1 1 1 > 1 1 0 1 1 1 3 > 21 gss_human_bak.seq 2007-05-06 22:04:51.888548 1 1 > 1 1 1 0 1 1 1 3 > 1293 RGD 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 9 Patents (nrdb) 2007-05-06 22:04:51.888548 1 1 1 > 1 1 0 1 1 1 3 > 15 BLOCKS 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 37 swissprot 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 2 GenBank (nrdb) 2007-05-06 22:04:51.888548 1 1 1 > 1 1 0 1 1 1 3 > 20 dbEST 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 166 Demeter 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 5 NBRF PIR (nrdb) 2007-05-06 22:04:51.888548 1 1 1 > 1 1 0 1 1 1 3 > 81 pir 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 95 tigr 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 1692 dbSTS 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 16 PRINTS 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 83 hssp 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 3009 GeneDB_Ddiscoideum 2007-05-06 22:04:51.888548 1 1 > 1 1 1 0 1 1 1 3 > 163 RepBase 4.03 vrtrep.ref 4.0.0 2007-05-06 22:04:51.888548 > 1 1 1 1 1 0 1 1 1 3 > 3017 GeneDB_Bparapertussis 2007-05-06 22:04:51.888548 1 > 1 1 1 1 0 1 1 1 3 > 87 wormpep 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 151 Plasmodium_RoosLab 2007-05-06 22:04:51.888548 1 1 > 1 1 1 0 1 1 1 3 > 3016 GeneDB_Bbronchiseptica 2007-05-06 22:04:51.888548 1 > 1 1 1 1 0 1 1 1 3 > 146 S. cerevisiae translated orfs 2007-05-06 22:04:51.888548 > 1 1 1 1 1 0 1 1 1 3 > 182 UCSC human draft genomic sequence (June 15 release) > 2007-05-06 22:04:51.888548 1 1 1 1 1 0 1 1 > 1 3 > 4 DDBJ (nrdb) 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 159 RepBase 4.03 plnrep.ref 4.0.0 2007-05-06 22:04:51.888548 > 1 1 1 1 1 0 1 1 1 3 > 105 zfin 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 190 TRRD 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 2094 cazy 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 22 GenBank 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 140 epd 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 3019 GeneDB_Styphi 2007-05-06 22:04:51.888548 1 1 1 > 1 1 0 1 1 1 3 > 145 C.elegans translated orfs 2007-05-06 22:04:51.888548 1 > 1 1 1 1 0 1 1 1 3 > 162 RepBase 4.03 simple.ref 3.1 2007-05-06 22:04:51.888548 > 1 1 1 1 1 0 1 1 1 3 > 169 JGI/ORNL contigs 2007-05-06 22:04:51.888548 1 1 > 1 1 1 0 1 1 1 3 > 88 dictydb 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 173 SCOP 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 793 Derisi Oligos 2007-05-06 22:04:51.888548 1 1 1 > 1 1 0 1 1 1 3 > 7 Swiss Prot (nrdb) 2007-05-06 22:04:51.888548 1 1 1 > 1 1 0 1 1 1 3 > 3007 GeneDB_Scerevisiae 2007-05-06 22:04:51.888548 1 1 > 1 1 1 0 1 1 1 3 > 160 RepBase 4.03 pseudo.ref 1.0.5 2007-05-06 22:04:51.888548 > 1 1 1 1 1 0 1 1 1 3 > 91 swiss-2dpage 2007-05-06 22:04:51.888548 1 1 1 > 1 1 0 1 1 1 3 > 129 imgt/hla 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 123 taxon 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 100 yepd 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 96 sgd 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 1092 LocusID 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 86 maizedb 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 142 ORNL_grailexp_mrna 2007-05-06 22:04:51.888548 1 1 > 1 1 1 0 1 1 1 3 > 992 NCBI RefSeq 2007-05-06 22:04:51.888548 1 1 1 > 1 1 0 1 1 1 3 > 3015 GeneDB_Tbrucei 2007-05-06 22:04:51.888548 1 1 1 > 1 1 0 1 1 1 3 > 147 TIGR mouse BAC ends 2007-05-06 22:04:51.888548 1 1 > 1 1 1 0 1 1 1 3 > 192 NCBI Malaria Genetics and Genome Project 2007-05-06 > 22:04:51.888548 1 1 1 1 1 0 1 1 1 3 > 99 gcrdb 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 11 General database Identifier (nrdb) 2007-05-06 > 22:04:51.888548 1 1 1 1 1 0 1 1 1 3 > 107 rebase 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 180 KEGG: Kyoto Encyclopedia of Genes and Genomes 2007-05-06 > 22:04:51.888548 1 1 1 1 1 0 1 1 1 3 > 3026 SWALL 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 158 RepBase 4.03 mamrep.ref 5.2.2 2007-05-06 22:04:51.888548 > 1 1 1 1 1 0 1 1 1 3 > 492 Mouse BAC fingerprint map 2007-05-06 22:04:51.888548 1 > 1 1 1 1 0 1 1 1 3 > 177 Incyte 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 134 gdb 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 93 pdb 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 3003 GO Process 2007-05-06 22:04:51.888548 1 1 1 > 1 1 0 1 1 1 3 > 167 Riethman lab. telomere sequences 2007-05-06 > 22:04:51.888548 1 1 1 1 1 0 1 1 1 3 > 3021 SPTR 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 141 ORNL_genscan_mrna 2007-05-06 22:04:51.888548 1 1 > 1 1 1 0 1 1 1 3 > 3023 TC 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 174 SMART 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 3020 GeneDB_Gmorsitans 2007-05-06 22:04:51.888548 1 1 > 1 1 1 0 1 1 1 3 > 139 rzpd 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 168 NCBI Reference Sequence (nrdb) 2007-05-06 22:04:51.888548 > 1 1 1 1 1 0 1 1 1 3 > 1294 COG 2007-05-06 22:04:51.888548 1 1 1 1 1 > 0 1 1 1 3 > 1592 Schwartz Lab Optical Mapping Data 2007-05-06 > 22:04:51.888548 1 1 1 1 1 0 1 1 1 3 > 19 Unigene_44 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 183 ORNL GenScan gene predictions 2007-05-06 22:04:51.888548 > 1 1 1 1 1 0 1 1 1 3 > 171 Pfam-B 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 176 MEROPS 2007-05-06 22:04:51.888548 1 1 1 1 > 1 0 1 1 1 3 > 150 Plasmodium_Sanger 2007-05-06 22:04:51.888548 1 1 > 1 1 1 0 1 1 1 3 > \. > > > Thanks in advance for any help you might provide ;-) > > Cheers, Alberto > > > > > davila wrote: >> Thanks Steve ! >> >> I will open the script and check it, however, is this a GUS plugin : >> "GUS::Supported::UnflattenBioperlFeatures::preprocess" ? if so, it is >> not available at $GUS_HOME or $PROJECT_HOME plugin directories: >> >> [davila1@kineto4 Supported]$ ls -lh >> $PROJECT_HOME/GUS/Supported/plugin/perl/ >> total 668K >> -rw-r--r-- 1 davila1 gus 3.3K May 3 17:12 CalculateAASequenceMolWt.pm >> -rw-r--r-- 1 davila1 gus 12K May 3 17:12 >> CalculateTranslatedAASequences.pm >> -rw-r--r-- 1 davila1 gus 38K May 3 17:12 dbEST.pm >> -rw-r--r-- 1 davila1 gus 7.5K May 3 17:12 ExtractTranscriptSequences.pm >> -rw-r--r-- 1 davila1 gus 28K May 3 17:12 InsertArrayDesignControl.pm >> -rw-r--r-- 1 davila1 gus 18K May 3 17:12 InsertAssayControl.pm >> -rw-r--r-- 1 davila1 gus 26K May 3 17:12 InsertBlastSimilarities.pm >> -rw-r--r-- 1 davila1 gus 5.0K May 3 17:12 InsertContact.pm >> -rw-r--r-- 1 davila1 gus 4.1K May 3 17:12 InsertExternalDatabase.pm >> -rw-r--r-- 1 davila1 gus 8.4K May 3 17:12 InsertExternalDatabaseRls.pm >> -rw-r--r-- 1 davila1 gus 14K May 3 17:12 InsertGene2Accession.pm >> -rw-r--r-- 1 davila1 gus 14K May 3 17:12 InsertGene2Go.pm >> -rw-r--r-- 1 davila1 gus 37K May 3 17:12 InsertGeneOntologyAssoc.pm >> -rw-r--r-- 1 davila1 gus 26K May 3 17:12 InsertGeneOntology.pm >> -rw-r--r-- 1 davila1 gus 4.2K May 3 17:12 InsertGOEvidenceCode.pm >> -rw-r--r-- 1 davila1 gus 14K May 3 17:12 InsertHomoloGene.pm >> -rw-r--r-- 1 davila1 gus 9.5K May 3 17:12 InsertOntologyEntry.pm >> -rw-r--r-- 1 davila1 gus 3.2K May 3 17:12 >> InsertOntologyRelationshipType.pm >> -rw-r--r-- 1 davila1 gus 16K May 3 17:12 >> InsertOntologyTermsAndRelationships.pm >> -rw-r--r-- 1 davila1 gus 3.1K May 3 17:12 InsertOntologyTermType.pm >> -rw-r--r-- 1 davila1 gus 30K May 3 17:12 InsertRadAnalysis.pm >> -rw-r--r-- 1 davila1 gus 4.1K May 3 17:12 InsertReviewStatus.pm >> -rw-r--r-- 1 davila1 gus 9.5K May 3 17:12 InsertSecondaryStructure.pm >> -rw-r--r-- 1 davila1 gus 41K May 3 17:12 InsertSequenceFeatures.pm >> -rw-r--r-- 1 davila1 gus 6.2K May 3 17:12 InsertSequenceFeaturesUndo.pm >> -rw-r--r-- 1 davila1 gus 4.3K May 3 17:12 InsertSequenceOntologyNew.pm >> -rw-r--r-- 1 davila1 gus 4.2K May 3 17:12 InsertSequenceOntologyOBO.pm >> -rw-r--r-- 1 davila1 gus 4.3K May 3 17:12 InsertSequenceOntology.pm >> -rw-r--r-- 1 davila1 gus 7.1K May 3 17:12 InsertTandemRepeatFeatures.pm >> -rw-r--r-- 1 davila1 gus 6.4K May 3 17:12 InsertTaxonAndTaxonName.pm >> -rw-r--r-- 1 davila1 gus 55K May 3 17:12 LoadArrayDesign.pm >> -rw-r--r-- 1 davila1 gus 46K May 3 17:12 LoadArrayResults.pm >> -rw-r--r-- 1 davila1 gus 23K May 3 17:12 LoadFastaSequences.pm >> -rw-r--r-- 1 davila1 gus 5.0K May 3 17:12 LoadGusXml.pm >> -rw-r--r-- 1 davila1 gus 25K May 3 17:12 LoadNRDB.pm >> -rw-r--r-- 1 davila1 gus 4.3K May 3 17:12 LoadRow.pm >> -rw-r--r-- 1 davila1 gus 13K May 3 17:12 LoadTaxon.pm >> drwxr-xr-x 3 davila1 gus 4.0K May 3 17:12 Test >> >> [davila1@kineto4 Supported]$ ls -lh >> $GUS_HOME/lib/perl/GUS/Supported/Plugin/ >> total 668K >> -rw-r--r-- 1 davila1 gus 3.3K May 3 17:12 CalculateAASequenceMolWt.pm >> -rw-r--r-- 1 davila1 gus 12K May 3 17:12 >> CalculateTranslatedAASequences.pm >> -rw-r--r-- 1 davila1 gus 38K May 3 17:12 dbEST.pm >> -rw-r--r-- 1 davila1 gus 7.5K May 3 17:12 ExtractTranscriptSequences.pm >> -rw-r--r-- 1 davila1 gus 28K May 3 17:12 InsertArrayDesignControl.pm >> -rw-r--r-- 1 davila1 gus 18K May 3 17:12 InsertAssayControl.pm >> -rw-r--r-- 1 davila1 gus 26K May 3 17:12 InsertBlastSimilarities.pm >> -rw-r--r-- 1 davila1 gus 5.0K May 3 17:12 InsertContact.pm >> -rw-r--r-- 1 davila1 gus 4.1K May 3 17:12 InsertExternalDatabase.pm >> -rw-r--r-- 1 davila1 gus 8.4K May 3 17:12 InsertExternalDatabaseRls.pm >> -rw-r--r-- 1 davila1 gus 14K May 3 17:12 InsertGene2Accession.pm >> -rw-r--r-- 1 davila1 gus 14K May 3 17:12 InsertGene2Go.pm >> -rw-r--r-- 1 davila1 gus 37K May 3 17:12 InsertGeneOntologyAssoc.pm >> -rw-r--r-- 1 davila1 gus 26K May 3 17:12 InsertGeneOntology.pm >> -rw-r--r-- 1 davila1 gus 4.2K May 3 17:12 InsertGOEvidenceCode.pm >> -rw-r--r-- 1 davila1 gus 14K May 3 17:12 InsertHomoloGene.pm >> -rw-r--r-- 1 davila1 gus 9.5K May 3 17:12 InsertOntologyEntry.pm >> -rw-r--r-- 1 davila1 gus 3.2K May 3 17:12 >> InsertOntologyRelationshipType.pm >> -rw-r--r-- 1 davila1 gus 16K May 3 17:12 >> InsertOntologyTermsAndRelationships.pm >> -rw-r--r-- 1 davila1 gus 3.1K May 3 17:12 InsertOntologyTermType.pm >> -rw-r--r-- 1 davila1 gus 30K May 3 17:12 InsertRadAnalysis.pm >> -rw-r--r-- 1 davila1 gus 4.1K May 3 17:12 InsertReviewStatus.pm >> -rw-r--r-- 1 davila1 gus 9.5K May 3 17:12 InsertSecondaryStructure.pm >> -rw-r--r-- 1 davila1 gus 41K May 3 17:12 InsertSequenceFeatures.pm >> -rw-r--r-- 1 davila1 gus 6.2K May 3 17:12 InsertSequenceFeaturesUndo.pm >> -rw-r--r-- 1 davila1 gus 4.3K May 3 17:12 InsertSequenceOntologyNew.pm >> -rw-r--r-- 1 davila1 gus 4.2K May 3 17:12 InsertSequenceOntologyOBO.pm >> -rw-r--r-- 1 davila1 gus 4.3K May 3 17:12 InsertSequenceOntology.pm >> -rw-r--r-- 1 davila1 gus 7.1K May 3 17:12 InsertTandemRepeatFeatures.pm >> -rw-r--r-- 1 davila1 gus 6.4K May 3 17:12 InsertTaxonAndTaxonName.pm >> -rw-r--r-- 1 davila1 gus 55K May 3 17:12 LoadArrayDesign.pm >> -rw-r--r-- 1 davila1 gus 46K May 3 17:12 LoadArrayResults.pm >> -rw-r--r-- 1 davila1 gus 23K May 3 17:12 LoadFastaSequences.pm >> -rw-r--r-- 1 davila1 gus 5.0K May 3 17:12 LoadGusXml.pm >> -rw-r--r-- 1 davila1 gus 25K May 3 17:12 LoadNRDB.pm >> -rw-r--r-- 1 davila1 gus 4.3K May 3 17:12 LoadRow.pm >> -rw-r--r-- 1 davila1 gus 13K May 3 17:12 LoadTaxon.pm >> drwxr-xr-x 2 davila1 gus 4.0K May 3 17:12 Test >> >> This is the part of the >> "$GUS_HOME/lib/perl/GUS/Supported/BioperlFeatMapperSet.pm" plugin >> originating the error: >> >> sub preprocessBioperlSeq { >> my ($self, $bioperlSeq) = @_; >> return unless $self->{bioperlSeqPreprocessor}; >> my $class = $self->{bioperlSeqPreprocessor}->{class}; >> >> eval { >> no strict "refs"; >> eval "require $class"; >> my $method = "${class}::preprocess"; >> &$method($bioperlSeq); ### Line 63 originating the error ##### >> }; >> >> my $err = $@; >> if ($err) { die "Can't run bioperlSeq preprocessor method >> '${class}::preprocess'. Error:\n $err\n"; } >> } >> >> Where I can find "$bioperlSeq" ? >> >> Thanks, Alberto >> >> >> >> -----Mensagem original----- >> De: Steve Fischer [mailto:sfi...@pc...] >> Enviada: sex 11-mai-07 3:01 >> Para: davila >> Cc: gus...@li...; mro...@cs...; >> cj...@fr... >> Assunto: Re: [GUSDEV] ISF error >> >> it looks as though it is trying to find the taxon of the sequence but is >> coming up with a null (see the method getTaxonId in the plugin). >> >> if the file contains all one species, you can provide that on the >> command line. >> >> otherwise, you'll need to look at the plugin code to see where in the >> files it is expecting to find that info and correct the files so that it >> is there. >> >> steve >> >> davila wrote: >> > >> > Hi Folks, could anyone help to debug this error ? >> > >> > ga GUS::Supported::Plugin::InsertSequenceFeatures --mapFile >> > $PROJECT_HOME/GUS/Supported/config/genbank2gus.xml --inputFileOrDir >> > /home/davila1/lguyanensis.gb --fileFormat genbank --extDbName GenBank >> > --extDbRlsVer 159.0 >> > Fri May 11 13:20:31 2007 DSN dbi:Pg:dbname=mydb >> > Fri May 11 13:20:31 2007 PLUGIN > >> GUS::Supported::Plugin::InsertSequenceFeatures >> > Fri May 11 13:20:31 2007 ARG algoinvo 1 >> > Fri May 11 13:20:31 2007 ARG bioperlTreeOutput 0 >> > Fri May 11 13:20:31 2007 ARG comment >> > Fri May 11 13:20:31 2007 ARG commit 0 >> > Fri May 11 13:20:31 2007 ARG debug 0 >> > Fri May 11 13:20:31 2007 ARG defaultOrganism >> > Fri May 11 13:20:31 2007 ARG extDbName GenBank >> > Fri May 11 13:20:31 2007 ARG extDbRlsVer 159.0 >> > Fri May 11 13:20:31 2007 ARG fileFormat genbank >> > Fri May 11 13:20:31 2007 ARG gff2GroupTag >> > Fri May 11 13:20:31 2007 ARG group >> > Fri May 11 13:20:31 2007 ARG gusconfigfile > >> $GUS_HOME/config/gus.config >> > Fri May 11 13:20:31 2007 ARG handlerExternalDbs >> > Fri May 11 13:20:31 2007 ARG help >> > Fri May 11 13:20:31 2007 ARG helpHTML >> > Fri May 11 13:20:31 2007 ARG inputFileExtension >> > Fri May 11 13:20:31 2007 ARG inputFileOrDir > >> /home/davila1/lguyanensis.gb >> > Fri May 11 13:20:31 2007 ARG mapFile >> > $PROJECT_HOME/GUS/Supported/config/genbank2gus.xml >> > Fri May 11 13:20:31 2007 ARG naSequenceSubclass >> > Fri May 11 13:20:31 2007 ARG organism >> > Fri May 11 13:20:31 2007 ARG project >> > Fri May 11 13:20:31 2007 ARG seqExtDbName >> > Fri May 11 13:20:31 2007 ARG seqExtDbRlsVer >> > Fri May 11 13:20:31 2007 ARG seqIdColumn source_id >> > Fri May 11 13:20:31 2007 ARG seqSoTerm >> > Fri May 11 13:20:31 2007 ARG seqType >> > Fri May 11 13:20:31 2007 ARG soCvsVersion >> > Fri May 11 13:20:31 2007 ARG sqlVerbose 0 >> > Fri May 11 13:20:31 2007 ARG testNumber >> > Fri May 11 13:20:31 2007 ARG user >> > Fri May 11 13:20:31 2007 ARG verbose 0 >> > Fri May 11 13:20:31 2007 ARG veryVerbose 0 >> > Fri May 11 13:20:31 2007 AlgInvocationId 61 >> > Fri May 11 13:20:31 2007 COMMIT commit off >> > Fri May 11 13:20:31 2007 Processing file >> > '/home/davila1/lguyanensis.gb'... >> > >> > ERROR: >> > Attempting to get id from cache with null name at >> > $GUS_HOME/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm >> line >> > 1103, <GEN32> line 65. >> > >> > STACK TRACE: >> > at $GUS_HOME/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm >> > line 1103 >> > > >> GUS::Supported::Plugin::InsertSequenceFeatures::getIdFromCache('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdd7f0)', >> >> > 'taxonNameCache', 'undef', 'GUS::Model::SRes::TaxonName', 'name', >> > 'taxon_id') called at >> > $GUS_HOME/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm >> line 998 >> > > >> GUS::Supported::Plugin::InsertSequenceFeatures::getTaxonId('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdd7f0)', >> >> > 'Bio::Seq::RichSeq=HASH(0x28fe130)') called at >> > $GUS_HOME/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm >> line 645 >> > > >> GUS::Supported::Plugin::InsertSequenceFeatures::constructNASequence('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdd7f0)', >> >> > 'Bio::Seq::RichSeq=HASH(0x28fe130)', 171) called at >> > $GUS_HOME/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm >> line 604 >> > > >> GUS::Supported::Plugin::InsertSequenceFeatures::bioperl2NASequence('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdd7f0)', >> >> > 'Bio::Seq::RichSeq=HASH(0x28fe130)', 171) called at >> > $GUS_HOME/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm >> line 574 >> > > >> GUS::Supported::Plugin::InsertSequenceFeatures::processSequence('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdd7f0)', >> >> > 'Bio::Seq::RichSeq=HASH(0x28fe130)', 171) called at >> > $GUS_HOME/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm >> line 361 >> > > >> GUS::Supported::Plugin::InsertSequenceFeatures::run('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdd7f0)', >> >> > 'HASH(0x20628f0)') called at >> > $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 580 >> > eval {...} called at >> > $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 569 >> > > >> GUS::PluginMgr::GusApplication::doMajorMode_RunOrReport('GUS::PluginMgr::GusApplication=HASH(0x5052a0)', >> >> > 'GUS::Supported::Plugin::InsertSequenceFeatures', 1) called at >> > $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 485 >> > > >> GUS::PluginMgr::GusApplication::doMajorMode_Run('GUS::PluginMgr::GusApplication=HASH(0x5052a0)', >> >> > 'GUS::Supported::Plugin::InsertSequenceFeatures') called at >> > $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 383 >> > > >> GUS::PluginMgr::GusApplication::doMajorMode('GUS::PluginMgr::GusApplication=HASH(0x5052a0)', >> >> > 'GUS::Supported::Plugin::InsertSequenceFeatures') called at >> > $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 292 >> > > >> GUS::PluginMgr::GusApplication::parseAndRun('GUS::PluginMgr::GusApplication=HASH(0x5052a0)', >> >> > 'ARRAY(0x505dd0)') called at $GUS_HOME/bin/ga line 11 >> > >> > >> > However, when I use --organism and --defaultOrganism the error >> changes: >> > >> > ga GUS::Supported::Plugin::InsertSequenceFeatures --mapFile >> > $PROJECT_HOME/GUS/Supported/config/genbank2gus.xml --inputFileOrDir >> > /home/davila1/lguyanensis.gb --fileFormat genbank --extDbName GenBank >> > --extDbRlsVer 159.0 --organism "Leishmania guyanensis" >> > --defaultOrganism "Leishmania guyanensis" --verbose >> > Fri May 11 13:21:54 2007 DSN dbi:Pg:dbname=mydb >> > Fri May 11 13:21:54 2007 PLUGIN > >> GUS::Supported::Plugin::InsertSequenceFeatures >> > Fri May 11 13:21:54 2007 ARG algoinvo 1 >> > Fri May 11 13:21:54 2007 ARG bioperlTreeOutput 0 >> > Fri May 11 13:21:54 2007 ARG comment >> > Fri May 11 13:21:54 2007 ARG commit 0 >> > Fri May 11 13:21:54 2007 ARG debug 0 >> > Fri May 11 13:21:54 2007 ARG defaultOrganism Leishmania >> > guyanensis >> > Fri May 11 13:21:54 2007 ARG extDbName GenBank >> > Fri May 11 13:21:54 2007 ARG extDbRlsVer 159.0 >> > Fri May 11 13:21:54 2007 ARG fileFormat genbank >> > Fri May 11 13:21:54 2007 ARG gff2GroupTag >> > Fri May 11 13:21:54 2007 ARG group >> > Fri May 11 13:21:54 2007 ARG gusconfigfile > >> $GUS_HOME/config/gus.config >> > Fri May 11 13:21:54 2007 ARG handlerExternalDbs >> > Fri May 11 13:21:54 2007 ARG help >> > Fri May 11 13:21:54 2007 ARG helpHTML >> > Fri May 11 13:21:54 2007 ARG inputFileExtension >> > Fri May 11 13:21:54 2007 ARG inputFileOrDir > >> /home/davila1/lguyanensis.gb >> > Fri May 11 13:21:54 2007 ARG mapFile >> > $PROJECT_HOME/GUS/Supported/config/genbank2gus.xml >> > Fri May 11 13:21:54 2007 ARG naSequenceSubclass >> > Fri May 11 13:21:54 2007 ARG organism Leishmania >> > guyanensis >> > Fri May 11 13:21:54 2007 ARG project >> > Fri May 11 13:21:54 2007 ARG seqExtDbName >> > Fri May 11 13:21:54 2007 ARG seqExtDbRlsVer >> > Fri May 11 13:21:54 2007 ARG seqIdColumn source_id >> > Fri May 11 13:21:54 2007 ARG seqSoTerm >> > Fri May 11 13:21:54 2007 ARG seqType >> > Fri May 11 13:21:54 2007 ARG soCvsVersion >> > Fri May 11 13:21:54 2007 ARG sqlVerbose 0 >> > Fri May 11 13:21:54 2007 ARG testNumber >> > Fri May 11 13:21:54 2007 ARG user >> > Fri May 11 13:21:54 2007 ARG verbose 1 >> > Fri May 11 13:21:54 2007 ARG veryVerbose 0 >> > Fri May 11 13:21:54 2007 AlgInvocationId 62 >> > Fri May 11 13:21:54 2007 COMMIT commit off >> > Fri May 11 13:21:54 2007 Processing file >> > '/home/davila1/lguyanensis.gb'... >> > >> > ERROR: >> > Can't run bioperlSeq preprocessor method >> > 'GUS::Supported::UnflattenBioperlFeatures::preprocess'. Error: >> > >> > ERROR: >> > Undefined subroutine >> > &GUS::Supported::UnflattenBioperlFeatures::preprocess called at >> > $GUS_HOME/lib/perl/GUS/Supported/BioperlFeatMapperSet.pm line 63, >> > <GEN32> line 65. >> > >> > STACK TRACE: >> > at >> > /system/GUS/gus_home/lib/perl/GUS/Supported/BioperlFeatMapperSet.pm >> > line 63 >> > eval {...} called at >> > $GUS_HOME/lib/perl/GUS/Supported/BioperlFeatMapperSet.pm line 59 >> > > >> GUS::Supported::BioperlFeatMapperSet::preprocessBioperlSeq('GUS::Supported::BioperlFeatMapperSet=HASH(0x207c9c0)', >> >> > 'Bio::Seq::RichSeq=HASH(0x28fe2d0)', >> > 'GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdd9b0)') >> > called at >> > $GUS_HOME/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm >> line 365 >> > > >> GUS::Supported::Plugin::InsertSequenceFeatures::run('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdd9b0)', >> >> > 'HASH(0x2062960)') called at >> > $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 580 >> > eval {...} called at >> > $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 569 >> > > >> GUS::PluginMgr::GusApplication::doMajorMode_RunOrReport('GUS::PluginMgr::GusApplication=HASH(0x5052a0)', >> >> > 'GUS::Supported::Plugin::InsertSequenceFeatures', 1) called at >> > $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 485 >> > > >> GUS::PluginMgr::GusApplication::doMajorMode_Run('GUS::PluginMgr::GusApplication=HASH(0x5052a0)', >> >> > 'GUS::Supported::Plugin::InsertSequenceFeatures') called at >> > $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 383 >> > > >> GUS::PluginMgr::GusApplication::doMajorMode('GUS::PluginMgr::GusApplication=HASH(0x5052a0)', >> >> > 'GUS::Supported::Plugin::InsertSequenceFeatures') called at >> > $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 292 >> > > >> GUS::PluginMgr::GusApplication::parseAndRun('GUS::PluginMgr::GusApplication=HASH(0x5052a0)', >> >> > 'ARRAY(0x505dd0)') called at $GUS_HOME/bin/ga line 11 >> > >> > >> > >> > STACK TRACE: >> > at $GUS_HOME/lib/perl/GUS/Supported/BioperlFeatMapperSet.pm line 67 >> > > >> GUS::Supported::BioperlFeatMapperSet::preprocessBioperlSeq('GUS::Supported::BioperlFeatMapperSet=HASH(0x207c9c0)', >> >> > 'Bio::Seq::RichSeq=HASH(0x28fe2d0)', >> > 'GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdd9b0)') >> > called at >> > $GUS_HOME/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm >> line 365 >> > > >> GUS::Supported::Plugin::InsertSequenceFeatures::run('GUS::Supported::Plugin::InsertSequenceFeatures=HASH(0xfdd9b0)', >> >> > 'HASH(0x2062960)') called at >> > $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 580 >> > eval {...} called at >> > $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 569 >> > > >> GUS::PluginMgr::GusApplication::doMajorMode_RunOrReport('GUS::PluginMgr::GusApplication=HASH(0x5052a0)', >> >> > 'GUS::Supported::Plugin::InsertSequenceFeatures', 1) called at >> > $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 485 >> > > >> GUS::PluginMgr::GusApplication::doMajorMode_Run('GUS::PluginMgr::GusApplication=HASH(0x5052a0)', >> >> > 'GUS::Supported::Plugin::InsertSequenceFeatures') called at >> > $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 383 >> > > >> GUS::PluginMgr::GusApplication::doMajorMode('GUS::PluginMgr::GusApplication=HASH(0x5052a0)', >> >> > 'GUS::Supported::Plugin::InsertSequenceFeatures') called at >> > $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 292 >> > > >> GUS::PluginMgr::GusApplication::parseAndRun('GUS::PluginMgr::GusApplication=HASH(0x5052a0)', >> >> > 'ARRAY(0x505dd0)') called at $GUS_HOME/bin/ga line 11 >> > >> > Today I re-ran the LoadTaxon plugin with a new version of the >> Taxonomy >> > (*.dmp) file from May 11 2007, apparently it does not make a new >> load, >> > but updates the respective Sres tables, anyway such as the first run >> > the re-run of LoadTaxon provided these warnings: >> > >> > Fri May 11 12:42:27 2007 Merging taxons >> > Fri May 11 12:42:27 2007 Doing merge and NCBI tax id '12' not >> > in database, skipping >> > Fri May 11 12:42:27 2007 Doing merge and NCBI tax id '30' not >> > in database, skipping >> > Fri May 11 12:42:27 2007 Doing merge and NCBI tax id '37' not >> > in database, skipping >> > Fri May 11 12:42:27 2007 Doing merge and NCBI tax id '46' not >> > in database, skipping >> > Fri May 11 12:42:27 2007 Doing merge and NCBI tax id '67' not >> > in database, skipping >> > Fri May 11 12:42:27 2007 Doing merge and NCBI tax id '76' not >> > in database, skipping >> > Fri May 11 12:42:27 2007 Doing merge and NCBI tax id '77' not >> > in database, skipping >> > Fri May 11 12:42:27 2007 Doing merge and NCBI tax id '79' not >> > in database, skipping >> > >> > What this exactly means ? >> > >> > The first time I ran LoadTaxon I got this log: >> > >> > tail LoadTaxon.log >> > Mon May 7 00:07:35 2007 Deleting TaxonNames when redundant >> > Mon May 7 00:07:39 2007 0 TaxonName entries deleted >> > >> > Mon May 7 00:07:42 2007 TIME 6653 sec (1:50:53) >> > Mon May 7 00:07:42 2007 RESULT 1 >> > Mon May 7 00:07:47 2007 Rows Written SRes.TaxonName: >> 485022 >> > Mon May 7 00:07:47 2007 Rows Written SRes.Taxon: 359527 >> > Mon May 7 00:07:47 2007 Rows Written SRes.GeneticCode: 18 >> > Mon May 7 00:07:47 2007 AlgInvocationId 7 >> > Mon May 7 00:07:47 2007 COMMIT commit on >> > >> > The second time: >> > >> > tail LoadTaxon_new.log >> > Fri May 11 13:07:47 2007 Deleting TaxonNames when redundant >> > Fri May 11 13:07:51 2007 288 TaxonName entries deleted >> > >> > Fri May 11 13:07:55 2007 TIME 1528 sec (0:25:28) >> > Fri May 11 13:07:55 2007 RESULT 1 >> > Fri May 11 13:07:56 2007 Rows Written SRes.TaxonName: 4023 >> > Fri May 11 13:07:56 2007 Rows Written SRes.Taxon: 2263 >> > Fri May 11 13:07:56 2007 Rows Written SRes.GeneticCode: 0 >> > Fri May 11 13:07:56 2007 AlgInvocationId 56 >> > Fri May 11 13:07:56 2007 COMMIT commit on >> > >> > I just checked the sres.taxonname and it has Leishmania guyanensis >> there: >> > >> > SELECT >> > >> "taxon_name_id","taxon_id","name","unique_name_variant","name_class","modification_date","row_project_id","row_alg_invocation_id" >> >> > FROM "sres"."taxonname" WHERE >> > "name" = 'Leishmania guyanensis'; >> > taxon_name_id | taxon_id | name | >> > unique_name_variant | name_class | modification_date | >> > row_project_id | row_alg_invocation_id >> > >> ---------------+----------+-----------------------+---------------------+-----------------+---------------------------+----------------+----------------------- >> >> > 369208 | 96595 | Leishmania guyanensis >> > | | scientific name | 2007-05-06 23:41:16.98889 >> > | 1 | 7 >> > (1 row) >> > >> > According to the log, it could be an >> > UnflattenBioperlFeatures::preprocess error too... >> > >> > Some of th GIs (L. guyanensis) I am trying to load with the ISF >> > pluging are: >> > >> > 104530485 >> > 60280340 >> > 110816091 >> > 76577720 >> > 76577719 >> > 76577718 >> > >> > Thanks for any help you might provide. >> > >> > Cheers, Alberto >> > >> >> >> ------------------------------------------------------------------------ >> >> ------------------------------------------------------------------------- >> >> This SF.net email is sponsored by DB2 Express >> Download DB2 Express C - the FREE version of DB2 express and take >> control of your XML. 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