From: davila <da...@io...> - 2007-05-11 17:36:07
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Hi Folks, could anyone help to debug this error ? ga GUS::Supported::Plugin::InsertSequenceFeatures --mapFile = $PROJECT_HOME/GUS/Supported/config/genbank2gus.xml --inputFileOrDir = /home/davila1/lguyanensis.gb --fileFormat genbank --extDbName GenBank = --extDbRlsVer 159.0 Fri May 11 13:20:31 2007 DSN dbi:Pg:dbname=3Dmydb Fri May 11 13:20:31 2007 PLUGIN = GUS::Supported::Plugin::InsertSequenceFeatures Fri May 11 13:20:31 2007 ARG algoinvo 1 Fri May 11 13:20:31 2007 ARG bioperlTreeOutput 0 Fri May 11 13:20:31 2007 ARG comment Fri May 11 13:20:31 2007 ARG commit 0 Fri May 11 13:20:31 2007 ARG debug 0 Fri May 11 13:20:31 2007 ARG defaultOrganism Fri May 11 13:20:31 2007 ARG extDbName GenBank Fri May 11 13:20:31 2007 ARG extDbRlsVer 159.0 Fri May 11 13:20:31 2007 ARG fileFormat genbank Fri May 11 13:20:31 2007 ARG gff2GroupTag Fri May 11 13:20:31 2007 ARG group Fri May 11 13:20:31 2007 ARG gusconfigfile = $GUS_HOME/config/gus.config Fri May 11 13:20:31 2007 ARG handlerExternalDbs Fri May 11 13:20:31 2007 ARG help Fri May 11 13:20:31 2007 ARG helpHTML Fri May 11 13:20:31 2007 ARG inputFileExtension Fri May 11 13:20:31 2007 ARG inputFileOrDir = /home/davila1/lguyanensis.gb Fri May 11 13:20:31 2007 ARG mapFile = $PROJECT_HOME/GUS/Supported/config/genbank2gus.xml Fri May 11 13:20:31 2007 ARG naSequenceSubclass Fri May 11 13:20:31 2007 ARG organism Fri May 11 13:20:31 2007 ARG project Fri May 11 13:20:31 2007 ARG seqExtDbName Fri May 11 13:20:31 2007 ARG seqExtDbRlsVer Fri May 11 13:20:31 2007 ARG seqIdColumn source_id Fri May 11 13:20:31 2007 ARG seqSoTerm Fri May 11 13:20:31 2007 ARG seqType Fri May 11 13:20:31 2007 ARG soCvsVersion Fri May 11 13:20:31 2007 ARG sqlVerbose 0 Fri May 11 13:20:31 2007 ARG testNumber Fri May 11 13:20:31 2007 ARG user Fri May 11 13:20:31 2007 ARG verbose 0 Fri May 11 13:20:31 2007 ARG veryVerbose 0 Fri May 11 13:20:31 2007 AlgInvocationId 61 Fri May 11 13:20:31 2007 COMMIT commit off Fri May 11 13:20:31 2007 Processing file = '/home/davila1/lguyanensis.gb'... ERROR: Attempting to get id from cache with null name at = $GUS_HOME/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm line = 1103, <GEN32> line 65. STACK TRACE: at $GUS_HOME/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm = line 1103 = GUS::Supported::Plugin::InsertSequenceFeatures::getIdFromCache('GUS::Supp= orted::Plugin::InsertSequenceFeatures=3DHASH(0xfdd7f0)', = 'taxonNameCache', 'undef', 'GUS::Model::SRes::TaxonName', 'name', = 'taxon_id') called at = $GUS_HOME/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm line = 998 = GUS::Supported::Plugin::InsertSequenceFeatures::getTaxonId('GUS::Supporte= d::Plugin::InsertSequenceFeatures=3DHASH(0xfdd7f0)', = 'Bio::Seq::RichSeq=3DHASH(0x28fe130)') called at = $GUS_HOME/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm line = 645 = GUS::Supported::Plugin::InsertSequenceFeatures::constructNASequence('GUS:= :Supported::Plugin::InsertSequenceFeatures=3DHASH(0xfdd7f0)', = 'Bio::Seq::RichSeq=3DHASH(0x28fe130)', 171) called at = $GUS_HOME/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm line = 604 = GUS::Supported::Plugin::InsertSequenceFeatures::bioperl2NASequence('GUS::= Supported::Plugin::InsertSequenceFeatures=3DHASH(0xfdd7f0)', = 'Bio::Seq::RichSeq=3DHASH(0x28fe130)', 171) called at = $GUS_HOME/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm line = 574 = GUS::Supported::Plugin::InsertSequenceFeatures::processSequence('GUS::Sup= ported::Plugin::InsertSequenceFeatures=3DHASH(0xfdd7f0)', = 'Bio::Seq::RichSeq=3DHASH(0x28fe130)', 171) called at = $GUS_HOME/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm line = 361 = GUS::Supported::Plugin::InsertSequenceFeatures::run('GUS::Supported::Plug= in::InsertSequenceFeatures=3DHASH(0xfdd7f0)', 'HASH(0x20628f0)') called = at $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 580 eval {...} called at = $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 569 = GUS::PluginMgr::GusApplication::doMajorMode_RunOrReport('GUS::PluginMgr::= GusApplication=3DHASH(0x5052a0)', = 'GUS::Supported::Plugin::InsertSequenceFeatures', 1) called at = $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 485 = GUS::PluginMgr::GusApplication::doMajorMode_Run('GUS::PluginMgr::GusAppli= cation=3DHASH(0x5052a0)', = 'GUS::Supported::Plugin::InsertSequenceFeatures') called at = $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 383 = GUS::PluginMgr::GusApplication::doMajorMode('GUS::PluginMgr::GusApplicati= on=3DHASH(0x5052a0)', 'GUS::Supported::Plugin::InsertSequenceFeatures') = called at $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 292 = GUS::PluginMgr::GusApplication::parseAndRun('GUS::PluginMgr::GusApplicati= on=3DHASH(0x5052a0)', 'ARRAY(0x505dd0)') called at $GUS_HOME/bin/ga line = 11 However, when I use --organism and --defaultOrganism the error changes: ga GUS::Supported::Plugin::InsertSequenceFeatures --mapFile = $PROJECT_HOME/GUS/Supported/config/genbank2gus.xml --inputFileOrDir = /home/davila1/lguyanensis.gb --fileFormat genbank --extDbName GenBank = --extDbRlsVer 159.0 --organism "Leishmania guyanensis" --defaultOrganism = "Leishmania guyanensis" --verbose Fri May 11 13:21:54 2007 DSN dbi:Pg:dbname=3Dmydb Fri May 11 13:21:54 2007 PLUGIN = GUS::Supported::Plugin::InsertSequenceFeatures Fri May 11 13:21:54 2007 ARG algoinvo 1 Fri May 11 13:21:54 2007 ARG bioperlTreeOutput 0 Fri May 11 13:21:54 2007 ARG comment Fri May 11 13:21:54 2007 ARG commit 0 Fri May 11 13:21:54 2007 ARG debug 0 Fri May 11 13:21:54 2007 ARG defaultOrganism Leishmania = guyanensis Fri May 11 13:21:54 2007 ARG extDbName GenBank Fri May 11 13:21:54 2007 ARG extDbRlsVer 159.0 Fri May 11 13:21:54 2007 ARG fileFormat genbank Fri May 11 13:21:54 2007 ARG gff2GroupTag Fri May 11 13:21:54 2007 ARG group Fri May 11 13:21:54 2007 ARG gusconfigfile = $GUS_HOME/config/gus.config Fri May 11 13:21:54 2007 ARG handlerExternalDbs Fri May 11 13:21:54 2007 ARG help Fri May 11 13:21:54 2007 ARG helpHTML Fri May 11 13:21:54 2007 ARG inputFileExtension Fri May 11 13:21:54 2007 ARG inputFileOrDir = /home/davila1/lguyanensis.gb Fri May 11 13:21:54 2007 ARG mapFile = $PROJECT_HOME/GUS/Supported/config/genbank2gus.xml Fri May 11 13:21:54 2007 ARG naSequenceSubclass Fri May 11 13:21:54 2007 ARG organism Leishmania = guyanensis Fri May 11 13:21:54 2007 ARG project Fri May 11 13:21:54 2007 ARG seqExtDbName Fri May 11 13:21:54 2007 ARG seqExtDbRlsVer Fri May 11 13:21:54 2007 ARG seqIdColumn source_id Fri May 11 13:21:54 2007 ARG seqSoTerm Fri May 11 13:21:54 2007 ARG seqType Fri May 11 13:21:54 2007 ARG soCvsVersion Fri May 11 13:21:54 2007 ARG sqlVerbose 0 Fri May 11 13:21:54 2007 ARG testNumber Fri May 11 13:21:54 2007 ARG user Fri May 11 13:21:54 2007 ARG verbose 1 Fri May 11 13:21:54 2007 ARG veryVerbose 0 Fri May 11 13:21:54 2007 AlgInvocationId 62 Fri May 11 13:21:54 2007 COMMIT commit off Fri May 11 13:21:54 2007 Processing file = '/home/davila1/lguyanensis.gb'... ERROR: Can't run bioperlSeq preprocessor method = 'GUS::Supported::UnflattenBioperlFeatures::preprocess'. Error: =20 ERROR: Undefined subroutine = &GUS::Supported::UnflattenBioperlFeatures::preprocess called at = $GUS_HOME/lib/perl/GUS/Supported/BioperlFeatMapperSet.pm line 63, = <GEN32> line 65. STACK TRACE: at /system/GUS/gus_home/lib/perl/GUS/Supported/BioperlFeatMapperSet.pm = line 63 eval {...} called at = $GUS_HOME/lib/perl/GUS/Supported/BioperlFeatMapperSet.pm line 59 = GUS::Supported::BioperlFeatMapperSet::preprocessBioperlSeq('GUS::Supporte= d::BioperlFeatMapperSet=3DHASH(0x207c9c0)', = 'Bio::Seq::RichSeq=3DHASH(0x28fe2d0)', = 'GUS::Supported::Plugin::InsertSequenceFeatures=3DHASH(0xfdd9b0)') = called at = $GUS_HOME/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm line = 365 = GUS::Supported::Plugin::InsertSequenceFeatures::run('GUS::Supported::Plug= in::InsertSequenceFeatures=3DHASH(0xfdd9b0)', 'HASH(0x2062960)') called = at $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 580 eval {...} called at = $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 569 = GUS::PluginMgr::GusApplication::doMajorMode_RunOrReport('GUS::PluginMgr::= GusApplication=3DHASH(0x5052a0)', = 'GUS::Supported::Plugin::InsertSequenceFeatures', 1) called at = $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 485 = GUS::PluginMgr::GusApplication::doMajorMode_Run('GUS::PluginMgr::GusAppli= cation=3DHASH(0x5052a0)', = 'GUS::Supported::Plugin::InsertSequenceFeatures') called at = $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 383 = GUS::PluginMgr::GusApplication::doMajorMode('GUS::PluginMgr::GusApplicati= on=3DHASH(0x5052a0)', 'GUS::Supported::Plugin::InsertSequenceFeatures') = called at $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 292 = GUS::PluginMgr::GusApplication::parseAndRun('GUS::PluginMgr::GusApplicati= on=3DHASH(0x5052a0)', 'ARRAY(0x505dd0)') called at $GUS_HOME/bin/ga line = 11 STACK TRACE: at $GUS_HOME/lib/perl/GUS/Supported/BioperlFeatMapperSet.pm line 67 = GUS::Supported::BioperlFeatMapperSet::preprocessBioperlSeq('GUS::Supporte= d::BioperlFeatMapperSet=3DHASH(0x207c9c0)', = 'Bio::Seq::RichSeq=3DHASH(0x28fe2d0)', = 'GUS::Supported::Plugin::InsertSequenceFeatures=3DHASH(0xfdd9b0)') = called at = $GUS_HOME/lib/perl/GUS/Supported/Plugin/InsertSequenceFeatures.pm line = 365 = GUS::Supported::Plugin::InsertSequenceFeatures::run('GUS::Supported::Plug= in::InsertSequenceFeatures=3DHASH(0xfdd9b0)', 'HASH(0x2062960)') called = at $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 580 eval {...} called at = $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 569 = GUS::PluginMgr::GusApplication::doMajorMode_RunOrReport('GUS::PluginMgr::= GusApplication=3DHASH(0x5052a0)', = 'GUS::Supported::Plugin::InsertSequenceFeatures', 1) called at = $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 485 = GUS::PluginMgr::GusApplication::doMajorMode_Run('GUS::PluginMgr::GusAppli= cation=3DHASH(0x5052a0)', = 'GUS::Supported::Plugin::InsertSequenceFeatures') called at = $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 383 = GUS::PluginMgr::GusApplication::doMajorMode('GUS::PluginMgr::GusApplicati= on=3DHASH(0x5052a0)', 'GUS::Supported::Plugin::InsertSequenceFeatures') = called at $GUS_HOME/lib/perl/GUS/PluginMgr/GusApplication.pm line 292 = GUS::PluginMgr::GusApplication::parseAndRun('GUS::PluginMgr::GusApplicati= on=3DHASH(0x5052a0)', 'ARRAY(0x505dd0)') called at $GUS_HOME/bin/ga line = 11 Today I re-ran the LoadTaxon plugin with a new version of the Taxonomy = (*.dmp) file from May 11 2007, apparently it does not make a new load, = but updates the respective Sres tables, anyway such as the first run the = re-run of LoadTaxon provided these warnings: Fri May 11 12:42:27 2007 Merging taxons Fri May 11 12:42:27 2007 Doing merge and NCBI tax id '12' not in = database, skipping Fri May 11 12:42:27 2007 Doing merge and NCBI tax id '30' not in = database, skipping Fri May 11 12:42:27 2007 Doing merge and NCBI tax id '37' not in = database, skipping Fri May 11 12:42:27 2007 Doing merge and NCBI tax id '46' not in = database, skipping Fri May 11 12:42:27 2007 Doing merge and NCBI tax id '67' not in = database, skipping Fri May 11 12:42:27 2007 Doing merge and NCBI tax id '76' not in = database, skipping Fri May 11 12:42:27 2007 Doing merge and NCBI tax id '77' not in = database, skipping Fri May 11 12:42:27 2007 Doing merge and NCBI tax id '79' not in = database, skipping What this exactly means ? The first time I ran LoadTaxon I got this log: tail LoadTaxon.log=20 Mon May 7 00:07:35 2007 Deleting TaxonNames when redundant Mon May 7 00:07:39 2007 0 TaxonName entries deleted Mon May 7 00:07:42 2007 TIME 6653 sec (1:50:53) Mon May 7 00:07:42 2007 RESULT 1 Mon May 7 00:07:47 2007 Rows Written SRes.TaxonName: 485022 Mon May 7 00:07:47 2007 Rows Written SRes.Taxon: 359527 Mon May 7 00:07:47 2007 Rows Written SRes.GeneticCode: 18 Mon May 7 00:07:47 2007 AlgInvocationId 7 Mon May 7 00:07:47 2007 COMMIT commit on The second time: tail LoadTaxon_new.log=20 Fri May 11 13:07:47 2007 Deleting TaxonNames when redundant Fri May 11 13:07:51 2007 288 TaxonName entries deleted Fri May 11 13:07:55 2007 TIME 1528 sec (0:25:28) Fri May 11 13:07:55 2007 RESULT 1 Fri May 11 13:07:56 2007 Rows Written SRes.TaxonName: 4023 Fri May 11 13:07:56 2007 Rows Written SRes.Taxon: 2263 Fri May 11 13:07:56 2007 Rows Written SRes.GeneticCode: 0 Fri May 11 13:07:56 2007 AlgInvocationId 56 Fri May 11 13:07:56 2007 COMMIT commit on I just checked the sres.taxonname and it has Leishmania guyanensis = there: SELECT = "taxon_name_id","taxon_id","name","unique_name_variant","name_class","mod= ification_date","row_project_id","row_alg_invocation_id" FROM = "sres"."taxonname" WHERE=20 "name" =3D 'Leishmania guyanensis'; taxon_name_id | taxon_id | name | unique_name_variant = | name_class | modification_date | row_project_id | = row_alg_invocation_id=20 ---------------+----------+-----------------------+---------------------+= -----------------+---------------------------+----------------+----------= ------------- 369208 | 96595 | Leishmania guyanensis | = | scientific name | 2007-05-06 23:41:16.98889 | 1 | = 7 (1 row) According to the log, it could be an = UnflattenBioperlFeatures::preprocess error too... Some of th GIs (L. guyanensis) I am trying to load with the ISF pluging = are: 104530485 60280340 110816091 76577720 76577719 76577718 Thanks for any help you might provide. Cheers, Alberto |