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From: Kumar, S. <San...@ng...> - 2006-05-02 15:24:19
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Hi Group,
I am having little problem with InsertSequenceFeature in our
environment.
It is loading all features from the genBank file except "intron"
feature. I tried to look into the code and found that it is all together
skipping the "intron" feature from genBank file. Is that the issue with
"BioPerl"?
Did any one face this issue earlier?
The version of bioperl in our environment is :1.5.0
And I am using very first version of GUS3.5. Do we have any subsequent
release like GUS3.5.X?=20
And the file I am trying to load is related to MICROSPORIDIA organism
(NC_003238.gbf).
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The genBank2gus.xml has entry for the intron like this:
<feature name=3D"intron" table=3D"DoTS::IntronFeature" so=3D"intron">
<qualifier name=3D"allele" lost=3D"true"/>
<qualifier name=3D"citation"/>
<qualifier name=3D"cons_splice"/>
<qualifier name=3D"evidence"/>
<qualifier name=3D"function"/>
<qualifier name=3D"gene" specialcase=3D"gene"/>
<qualifier name=3D"label"/>
<qualifier name=3D"locus_tag" column=3D"source_id"/>
<qualifier name=3D"map"/>
<qualifier name=3D"note" specialcase=3D"note"/>
<qualifier name=3D"number" column=3D"num"/>
<qualifier name=3D"old_locus_tag" lost=3D"true"/>
<qualifier name=3D"standard_name"/>
<qualifier name=3D"usedin"/>
<qualifier name=3D"db_xref" specialcase=3D"dbxref"/>
</feature>
And the intron entry in genBank file looks like this:
gene complement(52254..52444)
/db_xref=3D"GeneID:860376"
/locus_tag=3D"ECU09_0395"
CDS
join(complement(52254..52409),complement(52442..52444))
/db_xref=3D"GI:19173160"
/db_xref=3D"GeneID:860376"
/locus_tag=3D"ECU09_0395"
/codon_start=3D1
/protein_id=3D"NP_596963.1"
=20
/translation=3D"MGSRKTALVKTRLMRALKRNREIPAWKRMMKEHKGEYNRARRHW
RSKKLKIY"
/product=3D"60S RIBOSOMAL PROTEIN L39"
misc_feature
join(complement(52260..52409),complement(52442..52444))
/db_xref=3D"CDD:11875"
/locus_tag=3D"ECU09_0395"
/note=3D"RPL39; Region: Ribosomal protein L39E
[Translation, ribosomal structure and biogenesis]"
intron complement(52410..52441)
/locus_tag=3D"ECU09_0395"
/note=3D"intron L39"
Any help will be appreciated.
Thanks
Sanjeev Kumar
Northrop Grumman
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