From: <fab...@de...> - 2005-05-03 19:13:28
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Hello all, we are trying to load a genbank file into GUS by GBParser. We downloaded = a file from NCBI (sequences.gb) as a result search about "tripanosoma cruzi". Wh= en we executed the command to run the GBParser (ga GUS::Common::Plugin::GBParse= r --file=3Dsequences.gb --gbRel=3D145.0 --db_rel_id=3D62 --commit) we got t= he following outputs on the screen: ... ERR LINE=3D234512 unparsed line =20 /locus_tag=3D"SCO7809" ERR LINE=3D234513 unparsed line /note=3D"S= C8E7.06, possible TetR-family transcriptional ERR LINE=3D234514 unparsed line regulator,= len: 210 aa. Similar to several other members ERR LINE=3D234515 unparsed line of the Tet= R-family e.g. Streptomyces glaucescens ERR LINE=3D234516 unparsed line SW:TCMR_ST= RGA (EMBL:M80674) tetracenomycin C ERR LINE=3D234517 unparsed line transcript= ional repressor, TcmR (226 aa), fasta scores ERR LINE=3D234518 unparsed line opt: 187 z= -score: 235.6 E(): 1.2e-05 26.4% identity in 197 ERR LINE=3D234519 unparsed line aa overlap= and Streptomyces coelicolor TR:CAB88457 ERR LINE=3D234520 unparsed line (EMBL:AL35= 3815) putative TetR-family transcriptional ... and finally this, ERR LINE=3D235521 unparsed line RBS =20 complement(8667068..8667073) VLD CBIL::Bio::GenBank::Origin 1 Tue May 3 15:28:12 2005 STATUS N=3D1 ACC=3DNC_003888 =20 TOTAL_OBJECTS=3D17 Tue May 3 15:28:35 2005 Genbank entries inserted=3D 0; updated=3D= 0; total #(inserted::updated::deleted)=3D17:::: Tue May 3 15:28:35 2005 FAILURES Unable to process 2 entri= es. See gbparserFailures/ Tue May 3 15:28:35 2005 RESULT Genbank entries inserted=3D 0; u= pdated=3D 0; failed=3D 2 In the gbparserFailures/errors file has only this two lines: ------------------ entry number 1 ----------------- Can't call method "getSingleFeature" on an undefined value at /usr/local/GUS/gus_home/lib/perl/GUS/Common/Plugin/GBParser.pm line 311, = <GEN0> line 379982. ------------------ entry number 2 ----------------- Can't call method "getAccession" on an undefined value at /usr/local/GUS/gus_home/lib/perl/GUS/Common/Plugin/GBParser.pm line 223, = <GEN0> line 379983. Does anyone know what the problem is? Thanks a lot. ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. |