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From: Fernan A. <fe...@ii...> - 2006-09-27 01:25:27
|
Hi, we're having problems loading files where genes have more than one EC number (we're using the supported InsertSequenceFeatures plugin). Depending on how you view the problem it could be an issue of the plugin, the underlying schema, or both. Given that it is not unexpected that proteins could have more than one EC number, this should not be the standard behaviour (maybe give a warning but still continue loading?). Is this a known bug? Anyone has already worked around this? If not, I'll be submitting an issue to the tracker, Fernan |
From: <mro...@cs...> - 2006-09-26 20:42:43
|
Steve, I am sure that I am NOT using the "build command to get my files from project_home to gus_home" because I do not what you are talking about, could you please tell me how to run that command. Then if I still have a problem I will replace $Revision$ in the plugin file with a number like 5023 I hope you are enjoying some great pasta and pizza Thank you very much Michael |
From: <sfi...@pc...> - 2006-09-25 21:53:50
|
michael- our file server is down and i'm in italy, so i'm working a bit in the dark. i don't know why the file you are getting from the svn site doesn't have the revision correctly. you need to replace $Revision$ in the plugin file with a number like 5023 also, are you using the build command to get your files from project_home to gus_home? steve Quoting mro...@cs...: > Steve, > > Thank you very much for your help, here is the complete situation as of > today. > > > 1 - The data that i am trying to load into gus is Pseudomonas_aeruginosa" > found at > > ftp://ftp.ncbi.nlm.nih.gov/genomes/Bacteria/Pseudomonas_aeruginosa/ > > > 2 - today I run (using revision 1000) > ga GUS::Supported::Plugin::InsertSequenceFeatures > --mapFile $HOME/"pseudomonas aeruginosa"/pa01/genbank2gus.xml > --inputFileOrDir $HOME/"pseudomonas aeruginosa"/pa01/NC_002516.gbk > --fileFormat genbank > --extDbName "Pseudomonas_aeruginosa" > --extDbRlsVer "1000" > --soCvsVersion "1.41" > --commit > > and I get the same error as before: > > The plugin has an illegal cvs revision: '$Revision$'. If that doesn't > include a revision number, then the plugin has never been checked into > CVS. Please do so to give it an intial revision at /home/acrl-storage- > 1/gusdb/GUS/gus_home/lib/perl/GUS/PluginMgr/Plugin.pm line 347. > Database handle destroyed without explicit disconnect. > > > 3 - today I realized that I needed to do > > ga +create GUS::Supported::Plugin::InsertSequenceFeatures > > and I get the same error as above > > The plugin has an illegal cvs revision: '$Revision$'. If that doesn't > include a revision number, then the plugin has never been checked into > CVS. Please do so to give it an intial revision at /home/acrl-storage- > 1/gusdb/GUS/gus_home/lib/perl/GUS/PluginMgr/Plugin.pm line 347. > > > 4 - I found "InsertSequenceFeatures.pm" and the following plugins at: > > https://www.cbil.upenn.edu/svn/gus/GusAppFramework/trunk/Supported/plugin/perl/ > > Revision 5023: /GusAppFramework/trunk/Supported/plugin/perl > .. > CalculateAASequenceMolWt.pm > CalculateTranslatedAASequences.pm > ExtractTranscriptSequences.pm > InsertArrayDesignControl.pm > InsertAssayControl.pm > InsertBlastSimilarities.pm > InsertExternalDatabase.pm > InsertExternalDatabaseRls.pm > InsertGOEvidenceCode.pm > InsertGene2Accession.pm > InsertGene2Go.pm > InsertGeneOntology.pm > InsertGeneOntologyAssoc.pm > InsertHomoloGene.pm > InsertOntologyEntry.pm > InsertOntologyRelationshipType.pm > InsertOntologyTermType.pm > InsertOntologyTermsAndRelationships.pm > InsertRadAnalysis.pm > InsertReviewStatus.pm > InsertSecondaryStructure.pm > InsertSequenceFeatures.pm > InsertSequenceFeaturesUndo.pm > InsertSequenceOntology.pm > InsertSequenceOntologyNew.pm > InsertSequenceOntologyOBO.pm > InsertTandemRepeatFeatures.pm > InsertTaxonAndTaxonName.pm > LoadArrayDesign.pm > LoadArrayResults.pm > LoadFastaSequences.pm > LoadGusXml.pm > LoadNRDB.pm > LoadRow.pm > LoadTaxon.pm > dbEST.pm > > since they were NOT at > > /home/acrl-storage-1/gusdb/GUS/gus_home/lib/perl/GUS/Supported/Plugin > > I added them to that directory > > Then I executed InsertSequenceFeatures.pm --help and it worked, > > > At this time I am comming to the conclusion that just copying the missing > plugins into the > "/home/acrl-storage-1/gusdb/GUS/gus_home/lib/perl/GUS/Supported/Plugin" > directory is not enough since the error talks about > > "the plugin has never been checked into CVS. Please do so to give it an > intial revision at > /home/acrl-storage-1/gusdb/GUS/gus_home/lib/perl/GUS/PluginMgr/Plugin.pm > line 347." > > if i am correct, how can i check all the above plugins into CVS, and is > there something else that I need to do? > > > > Thank you very much for your help > > > > Michael Robinson > Bioinformatics Research Group (Biorg) > Florida International University > Miami, Florida, USA 33199 > > > > > > ------------------------------------------------------------------------- > Take Surveys. Earn Cash. Influence the Future of IT > Join SourceForge.net's Techsay panel and you'll get the chance to share your > opinions on IT & business topics through brief surveys -- and earn cash > http://www.techsay.com/default.php?page=join.php&p=sourceforge&CID=DEVDEV > _______________________________________________ > Gusdev-gusdev mailing list > Gus...@li... > https://lists.sourceforge.net/lists/listinfo/gusdev-gusdev > |
From: <mro...@cs...> - 2006-09-25 16:52:15
|
Steve, Thank you very much for your help, here is the complete situation as of today. 1 - The data that i am trying to load into gus is Pseudomonas_aeruginosa" found at ftp://ftp.ncbi.nlm.nih.gov/genomes/Bacteria/Pseudomonas_aeruginosa/ 2 - today I run (using revision 1000) ga GUS::Supported::Plugin::InsertSequenceFeatures --mapFile $HOME/"pseudomonas aeruginosa"/pa01/genbank2gus.xml --inputFileOrDir $HOME/"pseudomonas aeruginosa"/pa01/NC_002516.gbk --fileFormat genbank --extDbName "Pseudomonas_aeruginosa" --extDbRlsVer "1000" --soCvsVersion "1.41" --commit and I get the same error as before: The plugin has an illegal cvs revision: '$Revision$'. If that doesn't include a revision number, then the plugin has never been checked into CVS. Please do so to give it an intial revision at /home/acrl-storage- 1/gusdb/GUS/gus_home/lib/perl/GUS/PluginMgr/Plugin.pm line 347. Database handle destroyed without explicit disconnect. 3 - today I realized that I needed to do ga +create GUS::Supported::Plugin::InsertSequenceFeatures and I get the same error as above The plugin has an illegal cvs revision: '$Revision$'. If that doesn't include a revision number, then the plugin has never been checked into CVS. Please do so to give it an intial revision at /home/acrl-storage- 1/gusdb/GUS/gus_home/lib/perl/GUS/PluginMgr/Plugin.pm line 347. 4 - I found "InsertSequenceFeatures.pm" and the following plugins at: https://www.cbil.upenn.edu/svn/gus/GusAppFramework/trunk/Supported/plugin= /perl/ Revision 5023: /GusAppFramework/trunk/Supported/plugin/perl .. CalculateAASequenceMolWt.pm CalculateTranslatedAASequences.pm ExtractTranscriptSequences.pm InsertArrayDesignControl.pm InsertAssayControl.pm InsertBlastSimilarities.pm InsertExternalDatabase.pm InsertExternalDatabaseRls.pm InsertGOEvidenceCode.pm InsertGene2Accession.pm InsertGene2Go.pm InsertGeneOntology.pm InsertGeneOntologyAssoc.pm InsertHomoloGene.pm InsertOntologyEntry.pm InsertOntologyRelationshipType.pm InsertOntologyTermType.pm InsertOntologyTermsAndRelationships.pm InsertRadAnalysis.pm InsertReviewStatus.pm InsertSecondaryStructure.pm InsertSequenceFeatures.pm InsertSequenceFeaturesUndo.pm InsertSequenceOntology.pm InsertSequenceOntologyNew.pm InsertSequenceOntologyOBO.pm InsertTandemRepeatFeatures.pm InsertTaxonAndTaxonName.pm LoadArrayDesign.pm LoadArrayResults.pm LoadFastaSequences.pm LoadGusXml.pm LoadNRDB.pm LoadRow.pm LoadTaxon.pm dbEST.pm since they were NOT at /home/acrl-storage-1/gusdb/GUS/gus_home/lib/perl/GUS/Supported/Plugin I added them to that directory Then I executed InsertSequenceFeatures.pm --help and it worked, At this time I am comming to the conclusion that just copying the missing plugins into the "/home/acrl-storage-1/gusdb/GUS/gus_home/lib/perl/GUS/Supported/Plugin" directory is not enough since the error talks about "the plugin has never been checked into CVS. Please do so to give it an intial revision at /home/acrl-storage-1/gusdb/GUS/gus_home/lib/perl/GUS/PluginMgr/Plugin.pm line 347." if i am correct, how can i check all the above plugins into CVS, and is there something else that I need to do? Thank you very much for your help Michael Robinson Bioinformatics Research Group (Biorg) Florida International University Miami, Florida, USA 33199 |
From: <mro...@cs...> - 2006-09-15 22:50:06
|
Good afternoon; I am trying to run: ga GUS::Supported::Plugin::InsertSequenceFeatures --mapFile $HOME/"pseudomonas aeruginosa"/pa01/genbank2gus.xml --inputFileOrDir $HOME/"pseudomonas aeruginosa"/pa01/NC_002516.gbk --fileFormat genbank --extDbName "Pseudomonas_aeruginosa" --extDbRlsVer "06-16-2006" --soCvsVersion "1.41" --commit and I get the following error: The plugin has an illegal cvs revision: '$Revision$'. If that doesn't include a revision number, then the plugin has never bee= n checked into CVS. Please do so to give it an intial revision at /home/acrl-storage-1/gusdb/GUS/gus_home/lib/perl/GUS/PluginMgr/Plugin.pm line 347. Database handle destroyed without explicit disconnect. Line 347 of Plugin.pm has the following code: $self->{cvsRevision} =3D~ /Revision:\s+(\S+)\s+/ || die "The plugin has = an illegal cvs revision: '$self->{cvsRevision}'. If that doesn't include a revision number, then the plugin has never been checked into CVS. Pleas= e do so to give it an intial revision"; return $1; I have been searching the "archive emails" but I have not been able to find how to "check the plugin into CVS". Any help will very much appreciated. Michael Robinson Bioinformatics Research Group (Biorg) Florida International University Miami, Florida, USA 33199 |
From: Chris S. <sto...@pc...> - 2006-08-25 19:55:10
|
Hi All, Have just posted a powerpoint presentations on GUS at http:// www.cbil.upenn.edu/downloads/GUS/documentation/ You can get there from the the "informal notes and presentations" link in the Other Resources section at the bottom of http://www.gusdb.org/documentation.php BRCGUS06.ppt : Mini workshop on GUS for the Bioinformatics Resource Center retreat in Philadelphia (Feb 06). Cheers, Chris Chris Stoeckert, Ph.D. Research Associate Professor, Dept. of Genetics 1415 Blockley Hall, Center for Bioinformatics 423 Guardian Dr., University of Pennsylvania Philadelphia, PA 19104 Ph: 215-573-4409 FAX: 215-573-3111 |
From: John I. <io...@pc...> - 2006-08-22 19:04:50
|
Dr. Galea, Thank you for finding this solution. I'll test it and check it in to the repository. This will take a little while, since I'll have to install PostgreSQL and GUS. But that will be a good thing to have. John Iodice Computational Biology and Informatics Lab On Aug 18, 2006, at 9:19 AM, an...@ne... wrote: > Hello all, > I've stumbled upon a bug in the PostgreSQL implementation of the > Perl object > layer: when generating Perl objects, most childRelations are > missing even if > the PostgreSQL database contains the relevant FK constraint. > > The same bug shows up on two different PostgreSQL versions, namely > 8.1.4 and > 7.4.13. > > The problem lies in file '$PROJECT_HOME/GUS/ObjRelP/lib/perl/ > PostgreSQL.pm': > the subroutine 'tableChildRelationsSql' returns incorrect (schema > names are > reversed) and incomplete (filtering by schema name discards > relations to > external schemas) data. > > I'm attaching a file 'query.pl' that shows the problem on child > relations for > table 'GUS::Model::SRes::Contact', and a modified version of > PostgreSQL.pm with > a query that works correctly for me. > > Here is the output with the original query: > > TABLE GUS::Model::SRes::Contact > ---- Child relations from $db->getTableChildRelations > GUS::Model::SRes::BibliographicReference, contact_id, contact_id > GUS::Model::SRes::Contact, contact_id, affiliation_id > ------------ > > While this is the output with the modified SQL (incidentally, this > very same > output should be what is obtained from a working installation on > Oracle): > > TABLE GUS::Model::SRes::Contact > ---- Child relations from $db->getTableChildRelations > GUS::Model::Core::UserInfo, contact_id, contact_id > GUS::Model::DoTS::AASequenceEnzymeClass, contact_id, > ec_assignment_contact_id > GUS::Model::DoTS::Attribution, contact_id, contact_id > GUS::Model::DoTS::CloneSet, contact_id, contact_id > GUS::Model::DoTS::EST, contact_id, contact_id > GUS::Model::DoTS::NASequenceImp, contact_id, > sequencing_center_contact_id > GUS::Model::RAD::Analysis, contact_id, operator_id > GUS::Model::RAD::ArrayDesign, contact_id, manufacturer_id > GUS::Model::RAD::Assay, contact_id, operator_id > GUS::Model::RAD::Quantification, contact_id, operator_id > GUS::Model::SRes::BibliographicReference, contact_id, contact_id > GUS::Model::SRes::Contact, contact_id, affiliation_id > GUS::Model::Study::BioMaterialImp, contact_id, bio_source_provider_id > GUS::Model::Study::Study, contact_id, contact_id > ------------ > > Is there anyone using GUSDB on PostgreSQL who can confirm this > issue and test > that my solution does not break anything else? > > Thanks a lot in advance, > > Antonio Galea > > > > ---------------------------------------------------------------- > Net Wise webmail system - http://www.netwise.it > This message was sent using IMP, the Internet Messaging Program. > <PostgreSQL.pm> > <query.pl> |
From: Chris S. <sto...@pc...> - 2006-08-18 19:04:27
|
Since Steve answered the others ... >> 4- Table NALocation >> LOC_ORDER A feature (e.g., RNA, Gene) can have multiple locations on a sequence (e.g., contig, virtual sequence). This tells you the order of those multiple locations although it may be inferred from the starts and ends. >> IS_EXCLUDED don't remember offhand .. >> DB_NAME >> DB_IDENTIFIER probably should be database_release_id and source_id >> LITERAL_SEQUENCE again don't really remember but think it may be for helping specify the sequence the location marks by literally providing it >> REMARK comment field >> >> DEBUG_FIELD I'm guessing another type of comment field Chris On Aug 18, 2006, at 9:47 AM, Steve Fischer wrote: > ok, see below > > Ana Carol wrote: >> Hello all! >> >> I need to know the description/definition of some terms that don't >> have in >> gus schema browser. >> Can you help me??? >> 1- Table NASequence >> SEQUENCE_PIECE_ID >> > this is a foreign key to the SequencePiece view. this is used to tie > together pieces of sequence into a virtual sequence. the real-world > application is to model scaffolds. >> 2- Table Similarity >> BIT_SCORE_SUMMARY >> > this is designed to hold the bit score summary provided in a BLAST > result. it summarizes all the bit scores of the individual HSPs >> 3- Table SimilaritySpan >> BIT_SCORE >> > a bit score of a BLAST HSP >> 4- Table NALocation >> LOC_ORDER >> IS_EXCLUDED >> DB_NAME >> DB_IDENTIFIER >> LITERAL_SEQUENCE >> REMARK >> DEBUG_FIELD >> > i don't know.... i say ignore these, if you can. >> 5- Table NAProtein/NAGene >> IS_VERIFIED >> > this is set to 1 if a curator has reviewed the protein or gene >> I need this to put in my final dissertation of master's degree and >> i don't >> know biology very well, but I studied GUS more or less... >> >> Thanks in advance, >> Ana Carolina >> >> >> >> --------------------------------------------------------------------- >> --- >> >> --------------------------------------------------------------------- >> ---- >> Using Tomcat but need to do more? Need to support web services, >> security? >> Get stuff done quickly with pre-integrated technology to make your >> job easier >> Download IBM WebSphere Application Server v.1.0.1 based on Apache >> Geronimo >> http://sel.as-us.falkag.net/sel? >> cmd=lnk&kid=120709&bid=263057&dat=121642 >> --------------------------------------------------------------------- >> --- >> >> _______________________________________________ >> Gusdev-gusdev mailing list >> Gus...@li... >> https://lists.sourceforge.net/lists/listinfo/gusdev-gusdev >> > > ---------------------------------------------------------------------- > --- > Using Tomcat but need to do more? Need to support web services, > security? > Get stuff done quickly with pre-integrated technology to make your > job easier > Download IBM WebSphere Application Server v.1.0.1 based on Apache > Geronimo > http://sel.as-us.falkag.net/sel? > cmd=lnk&kid=120709&bid=263057&dat=121642 > _______________________________________________ > Gusdev-gusdev mailing list > Gus...@li... > https://lists.sourceforge.net/lists/listinfo/gusdev-gusdev |
From: Steve F. <sfi...@pc...> - 2006-08-18 13:48:10
|
ok, see below Ana Carol wrote: > Hello all! > > I need to know the description/definition of some terms that don't have in > gus schema browser. > Can you help me??? > 1- Table NASequence > SEQUENCE_PIECE_ID > this is a foreign key to the SequencePiece view. this is used to tie together pieces of sequence into a virtual sequence. the real-world application is to model scaffolds. > 2- Table Similarity > BIT_SCORE_SUMMARY > this is designed to hold the bit score summary provided in a BLAST result. it summarizes all the bit scores of the individual HSPs > 3- Table SimilaritySpan > BIT_SCORE > a bit score of a BLAST HSP > 4- Table NALocation > LOC_ORDER > IS_EXCLUDED > DB_NAME > DB_IDENTIFIER > LITERAL_SEQUENCE > REMARK > DEBUG_FIELD > i don't know.... i say ignore these, if you can. > 5- Table NAProtein/NAGene > IS_VERIFIED > this is set to 1 if a curator has reviewed the protein or gene > I need this to put in my final dissertation of master's degree and i don't > know biology very well, but I studied GUS more or less... > > Thanks in advance, > Ana Carolina > > > > ------------------------------------------------------------------------ > > ------------------------------------------------------------------------- > Using Tomcat but need to do more? Need to support web services, security? > Get stuff done quickly with pre-integrated technology to make your job easier > Download IBM WebSphere Application Server v.1.0.1 based on Apache Geronimo > http://sel.as-us.falkag.net/sel?cmd=lnk&kid=120709&bid=263057&dat=121642 > ------------------------------------------------------------------------ > > _______________________________________________ > Gusdev-gusdev mailing list > Gus...@li... > https://lists.sourceforge.net/lists/listinfo/gusdev-gusdev > |
From: <an...@ne...> - 2006-08-18 13:19:19
|
Hello all, I've stumbled upon a bug in the PostgreSQL implementation of the Perl obj= ect layer: when generating Perl objects, most childRelations are missing even= if the PostgreSQL database contains the relevant FK constraint. The same bug shows up on two different PostgreSQL versions, namely 8.1.4 = and 7.4.13. The problem lies in file '$PROJECT_HOME/GUS/ObjRelP/lib/perl/PostgreSQL.p= m': the subroutine 'tableChildRelationsSql' returns incorrect (schema names a= re reversed) and incomplete (filtering by schema name discards relations to external schemas) data. I'm attaching a file 'query.pl' that shows the problem on child relations= for table 'GUS::Model::SRes::Contact', and a modified version of PostgreSQL.p= m with a query that works correctly for me. Here is the output with the original query: TABLE GUS::Model::SRes::Contact ---- Child relations from $db->getTableChildRelations GUS::Model::SRes::BibliographicReference, contact_id, contact_id GUS::Model::SRes::Contact, contact_id, affiliation_id ------------ While this is the output with the modified SQL (incidentally, this very s= ame output should be what is obtained from a working installation on Oracle): TABLE GUS::Model::SRes::Contact ---- Child relations from $db->getTableChildRelations GUS::Model::Core::UserInfo, contact_id, contact_id GUS::Model::DoTS::AASequenceEnzymeClass, contact_id, ec_assignment_contac= t_id GUS::Model::DoTS::Attribution, contact_id, contact_id GUS::Model::DoTS::CloneSet, contact_id, contact_id GUS::Model::DoTS::EST, contact_id, contact_id GUS::Model::DoTS::NASequenceImp, contact_id, sequencing_center_contact_id GUS::Model::RAD::Analysis, contact_id, operator_id GUS::Model::RAD::ArrayDesign, contact_id, manufacturer_id GUS::Model::RAD::Assay, contact_id, operator_id GUS::Model::RAD::Quantification, contact_id, operator_id GUS::Model::SRes::BibliographicReference, contact_id, contact_id GUS::Model::SRes::Contact, contact_id, affiliation_id GUS::Model::Study::BioMaterialImp, contact_id, bio_source_provider_id GUS::Model::Study::Study, contact_id, contact_id ------------ Is there anyone using GUSDB on PostgreSQL who can confirm this issue and = test that my solution does not break anything else? Thanks a lot in advance, Antonio Galea ---------------------------------------------------------------- Net Wise webmail system - http://www.netwise.it This message was sent using IMP, the Internet Messaging Program. |
From: Ana C. <an...@ig...> - 2006-08-17 21:49:26
|
Hello all! I need to know the description/definition of some terms that don't have in gus schema browser. Can you help me??? 1- Table NASequence SEQUENCE_PIECE_ID 2- Table Similarity BIT_SCORE_SUMMARY 3- Table SimilaritySpan BIT_SCORE 4- Table NALocation LOC_ORDER IS_EXCLUDED DB_NAME DB_IDENTIFIER LITERAL_SEQUENCE REMARK DEBUG_FIELD 5- Table NAProtein/NAGene IS_VERIFIED I need this to put in my final dissertation of master's degree and i don't know biology very well, but I studied GUS more or less... Thanks in advance, Ana Carolina |
From: Steve F. <sfi...@pc...> - 2006-08-07 14:15:33
|
michael- gus gets its bioperl from the standard perl location. you install bioperl using cpan, and then it is available in the regular perl path. steve mro...@cs... wrote: > Hello folks; > > We have Gus 3.5 installed and have been able to to load the taxon data, > now while trying to use InsertSequenceFeatures.pl which uses BioPerl, we > have the following problem: > > We have 2 different versions of Perl, and Bioperl is installed in a > different computer, I have been looking for the config files that Gus uses > to look for Bioperl. > > I found and modified: > $GUS_HOME/config/install.pro > > but it seems that there are other file(s) that need to be modified > > Does anybody know where they are? > > > Cheers, > Michael Robinson > Bioinformatics Research Group (BioRG) > Florida International University > Miami FL 33199 > > > > > ------------------------------------------------------------------------- > Take Surveys. Earn Cash. Influence the Future of IT > Join SourceForge.net's Techsay panel and you'll get the chance to share your > opinions on IT & business topics through brief surveys -- and earn cash > http://www.techsay.com/default.php?page=join.php&p=sourceforge&CID=DEVDEV > _______________________________________________ > Gusdev-gusdev mailing list > Gus...@li... > https://lists.sourceforge.net/lists/listinfo/gusdev-gusdev > |
From: <mro...@cs...> - 2006-08-05 21:01:15
|
Hello folks; We have Gus 3.5 installed and have been able to to load the taxon data, now while trying to use InsertSequenceFeatures.pl which uses BioPerl, we have the following problem: We have 2 different versions of Perl, and Bioperl is installed in a different computer, I have been looking for the config files that Gus use= s to look for Bioperl. I found and modified: $GUS_HOME/config/install.pro but it seems that there are other file(s) that need to be modified Does anybody know where they are? Cheers, Michael Robinson Bioinformatics Research Group (BioRG) Florida International University Miami FL 33199 |
From: Roger U. <un...@sd...> - 2006-07-31 18:03:02
|
Hi, I have followed the installguide.pdf for GUS, and successfully completed all instructions in it ending with the following two commands: build GUS install -append -installDBSchema ga +meta --commit The problem is that I am unable to locate instructions/examples for populating GUS with data from external databases. If anyone can point me to some documentation on this, I would appreciate it. Thanks, Roger -- Roger Unwin un...@sd... |
From: marc j. <de...@ho...> - 2006-07-28 18:08:12
|
Hello, I'm trying to upgrade GUS to the latest release from 3.0. I'm trying to use DumpSchema.pl and @INC is way off. I modified the script to point to the correct library and it's still off. Where's the config file for the perl scripts that would allow me to make one change. Regards, Marc |
From: Mark S. H. <mh...@ug...> - 2006-07-16 03:17:41
|
Hi Adhemar, See comments inline... On Jul 11, 2006, at 12:50 PM, Adhemar Zerlotini Neto wrote: > I'm trying to upload some blast results following a manual created > by Pablo, from Jessica's Lab. at UGA. > > The problem is that the perl script > 'parseBlastFilesForSimilarity.pl' isn't working. > > Here is the error: > <extraneous error msg cut> > Analyzing > opening > /home/japonicum/JaponicumDB/PROJECTS_INFO/BLAST/SJest.VS.SJcontigs/ > SJest.VS.SJcontigs.PARSED > Can't locate object method "new" via package "Blast::BlastAnal" > (perhaps you forgot to load "Blast::BlastAnal"?) at > parseBlastFilesForSimilarity.pl line 81. > > This looks like the same problem as before - the script is looking for 'Blast::BlastAnal ' but the correct package name is CBIL::Bio::Blast::BlastAnal. > I would like to know how to fix this script or to download a new > version of the blast parse scripts. > We use the DJob framework for our blast execution and result parsing. https://cbil.upenn.edu/svn/gus/DJob/trunk/DistribJob/doc/DistribJob.html Within the DJob project is a blastSimilarity script. Your parseBlastFilesForSimilarity.pl script appears to be pretty much the same as DJob's blastSimilarity except blastSimilarity also executes the blast command in addition to parsing the result. I don't know of any other supported blast parsing script. The output of blastSimilarity can be loaded into GUS with GUS::Supported::Plugin::InsertBlastSimilarities. I believe that's the same for your parseBlastFilesForSimilarity.pl DJob is well documented and a very useful system if you use a compute cluster or have several types of analyses that you want to wrap in a pipeline. If you are running your blast on a single machine and just want to parse the results for loading into GUS then you will probably want to continue to use the parseBlastFilesForSimilarity script. In summary, fix the package name in your script and continue to use that. Email Pablo or me directly if you continue to have problems with parseBlastFilesForSimilarity. -Mark |
From: Adhemar Z. N. <ad...@cp...> - 2006-07-16 02:00:36
|
I'm trying to upload some blast results following a manual created by Pablo, from Jessica's Lab. at UGA. <br /><br />The problem is that the perl script '<font size=3D"2"><span style=3D"font-family: courier new,courier,monospace;">parseBlastFilesForSimilarity.pl' </span></font>isn't working.<br /><br />Here is the error:<br /><br /><font size=3D"2"><span style=3D"font-family: courier new,courier,monospace;">perl parseBlastFilesForSimilarity.pl --regex=3D'(\S+)'</span><br style=3D"font-family: courier new,courier,monospace;" /><span style=3D"font-family: courier new,courier,monospace;">--outputFile=3D/home/japonicum/JaponicumDB/PROJEC= TS_INFO/BLAST/SJest.VS.SJcontigs/SJest.VS.SJcontigs.PARSED</span><br style=3D"font-family: courier new,courier,monospace;" /><span style=3D"font-family: courier new,courier,monospace;">--dir=3D/home/japonicum/JaponicumDB/PROJECTS_INFO= /BLAST/SJest.VS.SJcontigs/SJest.VS.SJcontigs.RESULTS</span><br style=3D"font-family: courier new,courier,monospace;" /><span style=3D"font-family: courier new,courier,monospace;">Opening</span><br style=3D"font-family: courier new,courier,monospace;" /><span style=3D"font-family: courier new,courier,monospace;">/home/japonicum/JaponicumDB/PROJECTS_INFO/BLAST/S= Jest.VS.SJcontigs/SJest.VS.SJcontigs.PARSED</span><br style=3D"font-family: courier new,courier,monospace;" /><span style=3D"font-family: courier new,courier,monospace;">/home/japonicum/JaponicumDB/PROJECTS_INFO/BLAST/S= Jest.VS.SJcontigs/SJest.VS.SJcontigs.PARSED</span><br style=3D"font-family: courier new,courier,monospace;" /><span style=3D"font-family: courier new,courier,monospace;">is open</span><br style=3D"font-family: courier new,courier,monospace;" /><span style=3D"font-family: courier new,courier,monospace;">Cutoff parameters:</span><br style=3D"font-family: courier new,courier,monospace= ;" /><span style=3D"font-family: courier new,courier,monospace;">=A0P value: 1e-05</span><br style=3D"font-family: courier new,courier,monospace;" /><span style=3D"font-family: courier new,courier,monospace;">=A0Length: 10</span><br style=3D"font-family: courier new,courier,monospace;" /><spa= n style=3D"font-family: courier new,courier,monospace;">=A0Percent Identity= : 20</span><br style=3D"font-family: courier new,courier,monospace;" /><br style=3D"font-family: courier new,courier,monospace;" /><br style=3D"font-family: courier new,courier,monospace;" /><span style=3D"font-family: courier new,courier,monospace;">Analyzing</span><br style=3D"font-family: courier new,courier,monospace;" /><span style=3D"font-family: courier new,courier,monospace;">opening</span><br style=3D"font-family: courier new,courier,monospace;" /><span style=3D"font-family: courier new,courier,monospace;">/home/japonicum/JaponicumDB/PROJECTS_INFO/BLAST/S= Jest.VS.SJcontigs/SJest.VS.SJcontigs.PARSED</span><br style=3D"font-family: courier new,courier,monospace;" /><span style=3D"font-family: courier new,courier,monospace;">Can't locate object method "new" via package "Blast::BlastAnal"</span><br style=3D"font-family: courier new,courier,monospace;" /><span style=3D"font-family: courier new,courier,monospace;">(perhaps you forgot= to load "Blast::BlastAnal"?) at</span><br style=3D"font-family: courier new,courier,monospace;" /><span style=3D"font-family: courier new,courier,monospace;">parseBlastFilesForSimilarity.pl line 81.</span></font><br />=A0<br />=A0<br />I would like to know how to fix = this script or to download a new version of the blast parse scripts.<br /><br />Thanks,<br /><br />Ad.<br /><br /> |
From: Adhemar Z. N. <ad...@cp...> - 2006-07-16 01:51:49
|
I'm trying to upload some blast results following a manual created by Pablo, from Jessica's Lab. at UGA.<br /><br />The problem is that the per= l script 'parseBlastFilesForSimilarity.pl' isn't working.<br /><br />Here i= s the error:<br /><br /><font size=3D"2" style=3D"font-family: courier new,courier,monospace;">perl parseBlastFilesForSimilarity.pl --regex=3D'(\S+)'<br />--outputFile=3D/home/japonicum/JaponicumDB/PROJECTS_INFO/BLAST/SJest.VS= .SJcontigs/SJest.VS.SJcontigs.PARSED<br />--dir=3D/home/japonicum/JaponicumDB/PROJECTS_INFO/BLAST/SJest.VS.SJcont= igs/SJest.VS.SJcontigs.RESULTS<br />Opening<br />/home/japonicum/JaponicumDB/PROJECTS_INFO/BLAST/SJest.VS.SJcontigs/SJes= t.VS.SJcontigs.PARSED<br />/home/japonicum/JaponicumDB/PROJECTS_INFO/BLAST/SJest.VS.SJcontigs/SJes= t.VS.SJcontigs.PARSED<br />is open<br />Cutoff parameters:<br />=A0P value: 1e-05<br />=A0Length: = 10<br />=A0Percent Identity: 20<br /><br /><br />Analyzing<br />opening<br />/home/japonicum/JaponicumDB/PROJECTS_INFO/BLAST/SJest.VS.SJcontigs/SJes= t.VS.SJcontigs.PARSED<br />Can't locate object method "new" via package "Blast::BlastAnal"<br />(perhaps you forgot to load "Blast::BlastAnal"?) at<br />parseBlastFilesForSimilarity.pl line 81.</font><br />=A0<br />=A0<br />I would like to know how to fix th= is script or to download a new version of the blast parse scripts.<br /><br />Thanks,<br /><br />Ad.<br /><br /> |
From: Adhemar Z. N. <ad...@cp...> - 2006-07-13 11:25:51
|
<ad...@cp...><gus...@li...>Now it's working. I changed all 'Blast::BlastAnal' entries in the perl script file to 'CBIL::Bio::Blast::BlastAnal' and it worked. <br /><br />As you and Steve told it isn't a supported script, I wonder if you could publish the supported scripts and documentation for uploading blast results. <br /><b= r />Thanks,<br />Ad.<br /><br /><br /> <br /><br /><br />Adhemar Zerlotini Neto<br />Centro de Pesquisas Ren=E9 Rachou - FIOCRUZ<br />Av. Augusto de Lima 1715<br />BH MG 30190-002, Brazil<br />Phone: +55-31-3349-7785</gus...@li...></ad...@cp...o= cruz.br> |
From: <ju...@cs...> - 2006-07-12 20:45:46
|
pi...@pc... (Debbie) writes: > > LoadTaxonomy was written to update taxon_id in child tables prior to deleting > the row in taxon_id so I suspect that something else is happening, for example > a group_write or group_id issue. No, the problem is as I describe. Yes, obviously the intent behind LoadTaxon would have been to update taxon_id in all child tables first, but this is not happening. LoadTaxon is broken. > >> GUS::Model::SRes::Taxon=3DHASH(0x9ee9e54) > >> DBD::Oracle::db do failed: ORA-02292: integrity constraint > >> (DOTS.NASEQUENCEIMP_FK02) violated - child record found (DBD > >> ERROR:OCIStmtExecute) > >> [for Statement "DELETE FROM SRes.Taxon WHERE taxon_id = 525455 > >> "] at /home/oragus35/GUS/gus_home/lib/perl/GUS/ObjRelP/DbiDbHandle.pm > >> line 119, <MERGED> line 5476. > >> [...] |
From: <pi...@pc...> - 2006-07-12 19:06:04
|
LoadTaxonomy was written to update taxon_id in child tables prior to deleting the row in taxon_id so I suspect that something else is happening, for example a group_write or group_id issue. Debbie Quoting Josef Jurek <ju...@cs...>: > > "Kumar, Sanjeev" <San...@ng...> writes: >> >> But I am getting a new type of error message, provided below. Can some >> one pl. help me in understanding/resolving the error message. >> >> >> GUS::Model::SRes::Taxon=3DHASH(0xa166cf8) merged with Taxon_id =3D >> GUS::Model::SRes::Taxon=3DHASH(0x9ee9e54) >> DBD::Oracle::db do failed: ORA-02292: integrity constraint >> (DOTS.NASEQUENCEIMP_FK02) violated - child record found (DBD >> ERROR:OCIStmtExecute) >> [for Statement "DELETE FROM >> SRes.Taxon WHERE taxon_id =3D 525455 >> "] at /home/oragus35/GUS/gus_home/lib/perl/GUS/ObjRelP/DbiDbHandle.pm >> line 119, <MERGED> line 5476. >> [...] > > I ran into this very same error as well. What is happening > is that LoadTaxon is attempting to delete obsolete > taxonomy entries _however_ some of these taxonomy > entries are still referenced by the TAXON_ID field > of DoTS.NASequence. > > What LoadTaxon _should_ _do_ is update all places > in DoTS.NASequence/DoTS.AASequence (and possibly > other tables) so that obsolete taxonomy entries > are replace in the tables that reference > them _before_ LoadTaxon tries to delete them > from SRes.Taxon. > > My jury-rig (and I do mean jury-rig) was > to run a perl script on merged.dmp to > convert each data line to a SQL line which > might look like: > > update DoTS.ExternalAASequence set taxon_id = 255892 where taxon_id > = 256194; > > thus, updating the taxon_id fields. > > When it comes to updating Taxonomy information > LoadTaxon does not appear to be very robust. > > Josef > > > > Josef Jurek, Ph.D. > > Daphne Preuss Laboratory > Molecular Genetics and Cell Biology > The University of Chicago > ju...@cs... > > voice: (773) 702-9559 > fax: (773) 702-6648 > > > > > > > > > ------------------------------------------------------------------------- > Using Tomcat but need to do more? Need to support web services, security? > Get stuff done quickly with pre-integrated technology to make your job easier > Download IBM WebSphere Application Server v.1.0.1 based on Apache Geronimo > http://sel.as-us.falkag.net/sel?cmd=lnk&kid=120709&bid=263057&dat=121642 > _______________________________________________ > Gusdev-gusdev mailing list > Gus...@li... > https://lists.sourceforge.net/lists/listinfo/gusdev-gusdev > |
From: <jl...@vb...> - 2006-07-12 17:52:26
|
What I did to update taxonomy is to use SQL commnds. Assume you only have taxon_id on DoTS.Sequence table. 1. Backup your old taxon_id SQL>CREATE TABLE DoTS.NASequenceTaxonID AS select a.na_sequence_id, a.taxon_id, b.ncbi_tax_id from dots.nasequence a, sres.taxon b where a.taxon_id=b.taxon_id; 2. Delete taxon_id on DoTS.Sequence table SQL>update dots.nasequence set taxon_id = null; 3. Delete taxonomy data SQL>delete from sres.taxonname; SQL>delete from sres.taxon; 4.Run plugin to load new taxonomy data 5.Restore taxon_id on DoTS.Sequence table SQL>update dots.nasequence s set taxon_id=(select b.taxon_id from dots.nasequencetaxonid a, sres.taxon b where a.ncbi_tax_id=b.ncbi_tax_id and a.na_sequence_id=s.na_sequence_id); On Wed, July 12, 2006 1:31 pm, Josef Jurek wrote: > > "Kumar, Sanjeev" <San...@ng...> writes: > >> >> But I am getting a new type of error message, provided below. Can some >> one pl. help me in understanding/resolving the error message. >> >> >> GUS::Model::SRes::Taxon=3DHASH(0xa166cf8) merged with Taxon_id =3D >> GUS::Model::SRes::Taxon=3DHASH(0x9ee9e54) >> DBD::Oracle::db do failed: ORA-02292: integrity constraint >> (DOTS.NASEQUENCEIMP_FK02) violated - child record found (DBD >> ERROR:OCIStmtExecute) >> [for Statement "DELETE FROM >> SRes.Taxon WHERE taxon_id =3D 525455 >> "] at /home/oragus35/GUS/gus_home/lib/perl/GUS/ObjRelP/DbiDbHandle.pm >> line 119, <MERGED> line 5476. [...] >> > > I ran into this very same error as well. What is happening > is that LoadTaxon is attempting to delete obsolete taxonomy entries > _however_ some of these taxonomy > entries are still referenced by the TAXON_ID field of DoTS.NASequence. > > What LoadTaxon _should_ _do_ is update all places > in DoTS.NASequence/DoTS.AASequence (and possibly other tables) so that > obsolete taxonomy entries are replace in the tables that reference them > _before_ LoadTaxon tries to delete them > from SRes.Taxon. > > My jury-rig (and I do mean jury-rig) was > to run a perl script on merged.dmp to convert each data line to a SQL line > which might look like: > > update DoTS.ExternalAASequence set taxon_id = 255892 where taxon_id = > 256194; > > > thus, updating the taxon_id fields. > > When it comes to updating Taxonomy information > LoadTaxon does not appear to be very robust. > > > Josef > > > > > Josef Jurek, Ph.D. > > > Daphne Preuss Laboratory > Molecular Genetics and Cell Biology > The University of Chicago > ju...@cs... > > voice: (773) 702-9559 > fax: (773) 702-6648 > > > > > > > > > > ------------------------------------------------------------------------- > Using Tomcat but need to do more? Need to support web services, > security? Get stuff done quickly with pre-integrated technology to make > your job easier Download IBM WebSphere Application Server v.1.0.1 based on > Apache Geronimo > http://sel.as-us.falkag.net/sel?cmd=lnk&kid=120709&bid=263057&dat=121642 > _______________________________________________ > Gusdev-gusdev mailing list > Gus...@li... > https://lists.sourceforge.net/lists/listinfo/gusdev-gusdev > > |
From: Adhemar Z. N. <ad...@cp...> - 2006-07-12 17:45:07
|
Now it's working. I changed all 'Blast::BlastAnal' in the perl script file to 'CBIL::Bio::Blast::BlastAnal' and it worked. <br /><br />As you and Steve told it isn't a supported script, I wonder if you could publish the supported scripts and documentation for uploading blast results. <br /><br />Thanks,<br />Ad.<br /><br /><br /> |
From: <ju...@cs...> - 2006-07-12 17:31:55
|
"Kumar, Sanjeev" <San...@ng...> writes: > > But I am getting a new type of error message, provided below. Can some > one pl. help me in understanding/resolving the error message. > > > GUS::Model::SRes::Taxon=3DHASH(0xa166cf8) merged with Taxon_id =3D > GUS::Model::SRes::Taxon=3DHASH(0x9ee9e54) > DBD::Oracle::db do failed: ORA-02292: integrity constraint > (DOTS.NASEQUENCEIMP_FK02) violated - child record found (DBD ERROR:OCIStmtExecute) > [for Statement "DELETE FROM > SRes.Taxon WHERE taxon_id =3D 525455 > "] at /home/oragus35/GUS/gus_home/lib/perl/GUS/ObjRelP/DbiDbHandle.pm > line 119, <MERGED> line 5476. > [...] I ran into this very same error as well. What is happening is that LoadTaxon is attempting to delete obsolete taxonomy entries _however_ some of these taxonomy entries are still referenced by the TAXON_ID field of DoTS.NASequence. What LoadTaxon _should_ _do_ is update all places in DoTS.NASequence/DoTS.AASequence (and possibly other tables) so that obsolete taxonomy entries are replace in the tables that reference them _before_ LoadTaxon tries to delete them from SRes.Taxon. My jury-rig (and I do mean jury-rig) was to run a perl script on merged.dmp to convert each data line to a SQL line which might look like: update DoTS.ExternalAASequence set taxon_id = 255892 where taxon_id = 256194; thus, updating the taxon_id fields. When it comes to updating Taxonomy information LoadTaxon does not appear to be very robust. Josef Josef Jurek, Ph.D. Daphne Preuss Laboratory Molecular Genetics and Cell Biology The University of Chicago ju...@cs... voice: (773) 702-9559 fax: (773) 702-6648 |
From: <pi...@pc...> - 2006-07-12 17:07:04
|
Sorry, I read two messages and replied to the wrong one. Obviously this reply concerns the LoadTaxonomy plugin problem. Quoting pi...@pc...: > One possibility: > > You might not be in the right group or group write is not set properly on the > child table. If true, the plugin is not able to update the child row's > taxon_id > and therefore deleting that taxon row would violate a foreign key constraint. > Check the core.groupinfo for which group you're in and the group_write > field in > the child table. > > Debbie > > > > > Quoting Pablo Mendes <pa...@ug...>: > >> >> Hi Adhemar, >> it seems you need the BlastAnal.pm package >> >>> Can't locate object method "new" via package "Blast::BlastAnal" >>> (perhaps you forgot to >>> load "Blast::BlastAnal"?) at >> >> It is available in a CBIL tar: >> http://www.cbil.upenn.edu/downloads/CBIL/releases/ >> >> CBIL/Bio/lib/perl/Blast/BlastAnal.pm >> >> You might want to change/add a "use lib" to >> 'parseBlastFilesForSimilarity.pl' or to add the CBIL to your >> "classpath", changing the $PERL5LIB variable. >> (http://www.perl.com/doc/manual/html/pod/perlrun.html) >> >> >> Good luck, >> Pablo >> ----- Original Message ----- >> From: Adhemar Zerlotini Neto >> To: gus...@li... >> Sent: Wednesday, July 12, 2006 11:31 AM >> Subject: [GUSDEV] Blast Similarities >> >> >> I'm trying to upload some blast results following a manual created >> by Pablo, who works at Jessica's Lab. at UGA. >> >> The problem is that the perl script >> 'parseBlastFilesForSimilarity.pl' isn't working. >> >> Here is the error: >> >> perl parseBlastFilesForSimilarity.pl --regex='(\S+)' >> >> --outputFile=/home/japonicum/JaponicumDB/PROJECTS_INFO/BLAST/SJest.VS.SJcontigs/SJest.VS.SJcontigs.PARSED >> --dir=/home/japonicum/JaponicumDB/PROJECTS_INFO/BLAST/SJest.VS.SJcontigs/SJest.VS.SJcontigs.RESULTS >> Opening >> >> /home/japonicum/JaponicumDB/PROJECTS_INFO/BLAST/SJest.VS.SJcontigs/SJest.VS.SJcontigs.PARSED /home/japonicum/JaponicumDB/PROJECTS_INFO/BLAST/SJest.VS.SJcontigs/SJest.VS.SJcontigs.PARSED >> is >> open >> Cutoff parameters: >> P value: 1e-05 >> Length: 10 >> Percent Identity: 20 >> >> >> Analyzing >> opening >> >> /home/japonicum/JaponicumDB/PROJECTS_INFO/BLAST/SJest.VS.SJcontigs/SJest.VS.SJcontigs.PARSED Can't locate object method "new" via package "Blast::BlastAnal" (perhaps you forgot to load >> "Blast::BlastAnal"?) >> at >> parseBlastFilesForSimilarity.pl line 81. >> >> >> I would like to know how to fix this script or to download a new >> version of the blast parse scripts. >> >> Thanks, >> >> Ad. >> >> >> >> >> >> ------------------------------------------------------------------------------ >> >> >> >> ------------------------------------------------------------------------- >> Using Tomcat but need to do more? Need to support web services, security? >> Get stuff done quickly with pre-integrated technology to make your >> job easier >> Download IBM WebSphere Application Server v.1.0.1 based on Apache Geronimo >> http://sel.as-us.falkag.net/sel?cmd=lnk&kid=120709&bid=263057&dat=121642 >> >> >> >> ------------------------------------------------------------------------------ >> >> >> _______________________________________________ >> Gusdev-gusdev mailing list >> Gus...@li... >> https://lists.sourceforge.net/lists/listinfo/gusdev-gusdev >> > > > > > > ------------------------------------------------------------------------- > Using Tomcat but need to do more? Need to support web services, security? > Get stuff done quickly with pre-integrated technology to make your job easier > Download IBM WebSphere Application Server v.1.0.1 based on Apache Geronimo > http://sel.as-us.falkag.net/sel?cmd=lnk&kid=120709&bid=263057&dat=121642 > _______________________________________________ > Gusdev-gusdev mailing list > Gus...@li... > https://lists.sourceforge.net/lists/listinfo/gusdev-gusdev > |