From: Davis, A. C. <da...@mu...> - 2007-12-04 16:59:50
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I have been having problems getting the tile generator to generate tiles fo= r EST reads. I have them set up with EST DNA fasta files so that when zoom= ed in it displays the DNA with insertions/deletions and mismatches. It all= displays perfectly fine in gbrowse, but when I try to generate tiles, it h= angs and never even starts the entire landmark view. I tried disabling the= DNA display when zoomed in, but it still wouldnt generate. I'm wondering = if it has to do with having the GFF file set up to utilize the DNA fasta fi= le for the EST reads. For all of this, I loaded everything into database f= irst, then generated tiles after checking that it displayed properly in gbr= owse. Here are some example lines from my EST gff file scaffold_45 est match 753297 756343 . + . Tar= get EST:CACW5781.b1 1 905 scaffold_30 est match 563618 564742 . - . Tar= get EST:CACW6759.g1 1 742 scaffold_2 est match 1281895 1282741 . - . Tar= get EST:CACW11451.g1 1 863 scaffold_68 est match 160772 161720 . + . Tar= get EST:CACW4784.g2 1 725 scaffold_45 est HSP 753297 753324 . + . Tar= get EST:CACW5781.b1 1 28 scaffold_45 est HSP 753463 753501 . + . Tar= get EST:CACW5781.b1 29 67 scaffold_45 est HSP 753854 753979 . + . Tar= get EST:CACW5781.b1 68 193 scaffold_45 est HSP 754933 755079 . + . Tar= get EST:CACW5781.b1 194 340 scaffold_45 est HSP 755505 755688 . + . Tar= get EST:CACW5781.b1 341 524 scaffold_45 est HSP 755963 756343 . + . Tar= get EST:CACW5781.b1 525 905 scaffold_30 est HSP 563618 564293 . + . Tar= get EST:CACW6759.g1 1 675 scaffold_30 est HSP 564676 564742 . + . Tar= get EST:CACW6759.g1 676 742 scaffold_2 est HSP 1281895 1282741 . - . Tar= get EST:CACW11451.g1 1 863 scaffold_68 est HSP 161163 161720 . - . Tar= get EST:CACW4784.g2 1 560 scaffold_68 est HSP 160772 160936 . - . Tar= get EST:CACW4784.g2 561 725 And here is the track in my conf file: [EST] feature =3D match:est glyph =3D segments label_position =3D left draw_target =3D 1 show_mismatch =3D 1 fontcolor =3D blue mismatch_color =3D red true_target =3D 1 realign =3D 1 bgcolor =3D white fgcolor =3D black key =3D ESTs [EST:1000] label_position =3D top I have tried commenting out most of the track settings trying to make it ju= st a generic track, and it still doesnt work, which is why I think it has t= o do with the GFF file formatting. If anyone can possibly provide any insight as to why I can't get the tiles = to generate (or if it just isn't supported), I would greatly appreciate it. -Adam Davis |