From: Motokazu I. <m.i...@is...> - 2009-10-16 00:44:14
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I want to show exon-intron strucutre of mRNA. mRNA shape (gray arrow) can successfully be shown, but subfeatures (UTR and CDS) weren't shown on on it. Any subfeature-*.json file weren't created under data/tracks directory. Could you give me some advices ? GFF3 file and config file are belows. ** GFF3 file chr22 hg18_refGene gene 49288242 49293001 . - . ID=gene:NM_033200; Name=gene:NM_033200;Note=NM_033200 chr22 hg18_refGene mRNA 49288242 49293001 . - . ID=mRNA:NM_033200.1; Name=mRNA:NM_033200.1; Parent=gene:NM_033200; Index=1 chr22 hg18_refGene five_prime_UTR 49292971 49293001 . - . ID=five_prime_UTR:NM_033200.1.1; Name=five_prime_UTR:NM_033200.1.1; Parent=mRNA:NM_033200.1 chr22 hg18_refGene CDS 49292877 49292970 . - 0 ID=CDS:NM_033200.1.2; Name=CDS:NM_033200.1.2; Parent=mRNA:NM_033200.1 ** conf file { "description": "ucsc tracks", "db_adaptor": "Bio::DB::SeqFeature::Store", "db_args": { "-adaptor": "memory", "-dir": "stage/ucsc" }, "TRACK DEFAULTS": { "class": "feature", "autocomplete": "all" }, "tracks": [ { "track": "ucsc_gene", "key": "ucsc Gene Span", "feature": ["gene"], "autocomplete": "all", "class": "feature2", "category": "Gene Model features", "urlTemplate": "http://flybase.org/cgi-bin/fbidq.html?{load_id}", "extraData": {"load_id": "sub {shift->attributes(\"load_id\");}"} }, { "track": "ucsc_mRNA", "feature": ["mRNA"], "autocomplete": "alias", "subfeatures": true, "key": "ucsc mRNA", "class": "transcript", "subfeature_classes": { "CDS": "transcript-CDS", "five_prime_UTR": "transcript-five_prime_UTR", "three_prime_UTR": "transcript-three_prime_UTR" }, "arrowheadClass": "transcript-arrowhead", "clientConfig": { "histScale":5 }, "urlTemplate": "http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term={load_id}", "extraData": {"load_id": "sub {shift->attributes(\"load_id\");}"} }, { "track": "ucsc_snp", "feature": ["point_mutation"], "key": "ucsc Mutation: point mutation", "class": "feature", "category": "Mapped features" } ] } -- Osaka University Graduate School of Information Science and Technology Motokazu Ishikawa E-mail: m.i...@is... Web: http://www-mats.ist.osaka-u.ac.jp/ |