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From: Scott C. <sc...@sc...> - 2019-08-16 18:37:36
|
Hi Ahmet, Let me start with this: display and data storage are completely separate; you can have all of the data in a single directory if you want, and still have them display organized by lab or dataset (though of course, it might make life easier for you if you have them mirror each other). But the point is, if you have them laid out one way in the display and decide it doesn't work well for your users, you can change the display without having to change the underlying file system. I imagine this flexibility is at the root of your observation that documentation are not clear in what you want to know. I suppose what I would do if I were setting this up would be to create dataset directories where the files are stored, plus a json file that defines the tracks associated with that dataset. Then in the main trackList.json file, you can use include directives to pull in all of the configurations for the different datasets. The only possible downside to this is that it will require several "extra" http GETs to fetch each of the include files. Depending on how many include files you have, it might be a noticeable slowdown on initial startup. If that happens, you could write a little script that combines all of the include files into a single trackList.json for your production site. I do that for the main C. elegans JBrowse instance at WormBase because I would have to do includes for about 70 files, causing the load time to go up by 5 or 10 seconds. The other species at WormBase though just use the includes because they have significantly fewer tracks. The script that does the "bundling" of the various include files is here: https://github.com/WormBase/website-genome-browsers/blob/jbrowse-staging/jbrowse/bin/bundle_track_config.pl A trackList.json file that uses includes looks like this: https://www.wormbase.org/tools/genome/jbrowse-simple/data/b_malayi_PRJNA10729/trackList.json and an include file looks like this: https://www.wormbase.org/tools/genome/jbrowse-simple/data/b_malayi_PRJNA10729/includes/genes.json (on a side note--this is one of the things that I really love about JBrowse: the configuration files for public sites are easily viewable, so if you find a display you really like, you can look at how they did it.) Finally, to group the tracks together by dataset, you can make use of the "category" attribute and the default hierarchical track selector ( http://jbrowse.org/docs/track_selectors.html). You can also make subcategories by including a forward slash in the name of the category, which will result in a "sub-box" in the track selector. Ok, that's enough to get you started :-) Please let us know if you have more questions. Scott On Fri, Aug 16, 2019 at 10:44 AM Ozturk, Ahmet <Ahm...@um...> wrote: > Hello, > > I’m trying to set up JBrowse for the data in our lab for around 200 > samples. I’m working on the documentation, however, some parts are not > clear and I have some questions about the organization of data and conf > files. What is the best practice for adding many tracks, grouped by > researchers or datasets? I want people in the lab to compare their data to > others. Is it a good practice to create folders for each researcher, and > subfolders for each dataset containing 2 or more bam files and create > subcategories in the main conf file? Also, is there a way to create > individual conf files for each dataset/subfolder and include those in the > main conf file so that it’s tidy and easy to maintain? > > Thanks, Best, > > > Ahmet > _______________________________________________ > Gmod-ajax mailing list > Gmo...@li... > https://lists.sourceforge.net/lists/listinfo/gmod-ajax > -- ------------------------------------------------------------------------ Scott Cain, Ph. D. scott at scottcain dot net GMOD Coordinator (http://gmod.org/) 216-392-3087 Ontario Institute for Cancer Research |
From: Ozturk, A. <Ahm...@um...> - 2019-08-16 12:44:13
|
Hello, I’m trying to set up JBrowse for the data in our lab for around 200 samples. I’m working on the documentation, however, some parts are not clear and I have some questions about the organization of data and conf files. What is the best practice for adding many tracks, grouped by researchers or datasets? I want people in the lab to compare their data to others. Is it a good practice to create folders for each researcher, and subfolders for each dataset containing 2 or more bam files and create subcategories in the main conf file? Also, is there a way to create individual conf files for each dataset/subfolder and include those in the main conf file so that it’s tidy and easy to maintain? Thanks, Best, Ahmet |
From: Scott C. <sc...@sc...> - 2019-08-13 19:06:01
|
Hi Chin-Rang, That's good news--the only downside to using the non-dev version is that you can't install plugins if there are any you want--but that's a problem for another day :-) Scott On Tue, Aug 13, 2019 at 11:55 AM Yang, Chin-Rang (NIH/NHLBI) [E] < chi...@ni...> wrote: > Yes, it is working now with non-dev version. > > Thank you very much. > > > > Chin-Rang > > > > > > *From:* Scott Cain <sc...@sc...> > *Sent:* Tuesday, August 13, 2019 2:26 PM > *To:* Yang, Chin-Rang (NIH/NHLBI) [E] <chi...@ni...> > *Subject:* Re: [Gmod-ajax] jBrowse set up issue > > > > Hi Chin-Rang, > > > > The trick with sorting out a 404 error is to examine the url it's trying > to fetch and then figure out why the file it's looking for isn't where it > expects. If you have jbrowse-dev, you'll need to run setup.sh (oh, which > probably won't work on Windows) to generate the javascript bundle files. I > believe if you get the non-dev version, those files are already generated. > > > > Scott > > > > > > On Tue, Aug 13, 2019 at 11:24 AM Scott Cain <sc...@sc...> wrote: > > > > > > On Tue, Aug 13, 2019 at 11:00 AM Yang, Chin-Rang (NIH/NHLBI) [E] < > chi...@ni...> wrote: > > Hi Scott, > > > > Thank you for your prompt reply. > > > > Yes, my plan is to setup a web server for others to access my data in the > future. > > I was thinking to test it out in a local machine first. > > > > The error message I found from the Chrome browser is > > “Failed to load resource: the server responded with a status > main.bundle.js:1 of 404 (Not Found)” > > > > Thank you very much for your advice. > > I will talk to our IT people to see how to get an access for a web server > as well. > > > > > > Chin-Rang > > > > *From:* Scott Cain <sc...@sc...> > *Sent:* Tuesday, August 13, 2019 1:46 PM > *To:* Yang, Chin-Rang (NIH/NHLBI) [E] <chi...@ni...> > *Cc:* gmo...@li... > *Subject:* Re: [Gmod-ajax] jBrowse set up issue > > > > Hi Chin-Rang, > > > > I have a few suggestions: First, if you are only looking to display your > RNASeq reads locally, you'll probably find it a lot easier to use the > JBrowse desktop application: https://jbrowse.org/docs/jbrowse_desktop.html > (download at http://jbrowse.org/blog/2019/07/11/jbrowse-1-16-6.html). > It's JBrowse running in an Electron wrapper, so you don't have to deal with > a webserver at all. > > > > On the other hand if your goal is to get a server running so you can share > it with lab mates or the like, you can continue down the road using a > webserver. I have no experience trying to set up JBrowse on a Windows > machine. While the webserver part should work fine, I would guess that > you're likely to run into problems running the processing perl scripts like > prepare-refseqs.pl and flatfile-to-json.pl, but that's a step or two away > at the moment. To address your immediate problem, please open your > javascript console to see if there are any errors that might help us figure > out what's going on. > > > > Good luck, > > Scott > > > > > > On Tue, Aug 13, 2019 at 10:07 AM Yang, Chin-Rang (NIH/NHLBI) [E] via > Gmod-ajax <gmo...@li...> wrote: > > Hi, > > > > I am trying to setup jBrowse on a Apache server at the local Windows 10 > workstation to display my RNA-Seq data. > > > > The Apache server is running OK. Test web page shows “It works”. > > > > I put the Jbrowse folder in the Apache htdocs folder. > > > > When I run > > http://localhost/jbrowse/ > > the web page shows “ Loading …” > > > > I tried the “volvox” example, get tracks.conf, volvox.fa, volvox.fa.fai in > the data folder. > > Then run > > http://localhost/jbrowse/?data=data > > the web page shows “Loading …”, but nothing loaded … > > > > I am wondering if other people have the similar issue. > > Do I need to set jbrowse up in a really working web server, not a local > one? > > > > Please advise. Thank you. > > > > Chin-Rang > > > > _______________________________________________ > Gmod-ajax mailing list > Gmo...@li... > https://lists.sourceforge.net/lists/listinfo/gmod-ajax > > > > -- > > ------------------------------------------------------------------------ > Scott Cain, Ph. D. scott at scottcain > dot net > GMOD Coordinator (http://gmod.org/) 216-392-3087 > Ontario Institute for Cancer Research > > > > -- > > ------------------------------------------------------------------------ > Scott Cain, Ph. D. scott at scottcain > dot net > GMOD Coordinator (http://gmod.org/) 216-392-3087 > Ontario Institute for Cancer Research > > > > -- > > ------------------------------------------------------------------------ > Scott Cain, Ph. D. scott at scottcain > dot net > GMOD Coordinator (http://gmod.org/) 216-392-3087 > Ontario Institute for Cancer Research > -- ------------------------------------------------------------------------ Scott Cain, Ph. D. scott at scottcain dot net GMOD Coordinator (http://gmod.org/) 216-392-3087 Ontario Institute for Cancer Research |
From: Scott C. <sc...@sc...> - 2019-08-13 18:24:30
|
On Tue, Aug 13, 2019 at 11:00 AM Yang, Chin-Rang (NIH/NHLBI) [E] < chi...@ni...> wrote: > Hi Scott, > > > > Thank you for your prompt reply. > > > > Yes, my plan is to setup a web server for others to access my data in the > future. > > I was thinking to test it out in a local machine first. > > > > The error message I found from the Chrome browser is > > “Failed to load resource: the server responded with a status > main.bundle.js:1 of 404 (Not Found)” > > > > Thank you very much for your advice. > > I will talk to our IT people to see how to get an access for a web server > as well. > > > > > > Chin-Rang > > > > *From:* Scott Cain <sc...@sc...> > *Sent:* Tuesday, August 13, 2019 1:46 PM > *To:* Yang, Chin-Rang (NIH/NHLBI) [E] <chi...@ni...> > *Cc:* gmo...@li... > *Subject:* Re: [Gmod-ajax] jBrowse set up issue > > > > Hi Chin-Rang, > > > > I have a few suggestions: First, if you are only looking to display your > RNASeq reads locally, you'll probably find it a lot easier to use the > JBrowse desktop application: https://jbrowse.org/docs/jbrowse_desktop.html > (download at http://jbrowse.org/blog/2019/07/11/jbrowse-1-16-6.html). > It's JBrowse running in an Electron wrapper, so you don't have to deal with > a webserver at all. > > > > On the other hand if your goal is to get a server running so you can share > it with lab mates or the like, you can continue down the road using a > webserver. I have no experience trying to set up JBrowse on a Windows > machine. While the webserver part should work fine, I would guess that > you're likely to run into problems running the processing perl scripts like > prepare-refseqs.pl and flatfile-to-json.pl, but that's a step or two away > at the moment. To address your immediate problem, please open your > javascript console to see if there are any errors that might help us figure > out what's going on. > > > > Good luck, > > Scott > > > > > > On Tue, Aug 13, 2019 at 10:07 AM Yang, Chin-Rang (NIH/NHLBI) [E] via > Gmod-ajax <gmo...@li...> wrote: > > Hi, > > > > I am trying to setup jBrowse on a Apache server at the local Windows 10 > workstation to display my RNA-Seq data. > > > > The Apache server is running OK. Test web page shows “It works”. > > > > I put the Jbrowse folder in the Apache htdocs folder. > > > > When I run > > http://localhost/jbrowse/ > > the web page shows “ Loading …” > > > > I tried the “volvox” example, get tracks.conf, volvox.fa, volvox.fa.fai in > the data folder. > > Then run > > http://localhost/jbrowse/?data=data > > the web page shows “Loading …”, but nothing loaded … > > > > I am wondering if other people have the similar issue. > > Do I need to set jbrowse up in a really working web server, not a local > one? > > > > Please advise. Thank you. > > > > Chin-Rang > > > > _______________________________________________ > Gmod-ajax mailing list > Gmo...@li... > https://lists.sourceforge.net/lists/listinfo/gmod-ajax > > > > -- > > ------------------------------------------------------------------------ > Scott Cain, Ph. D. scott at scottcain > dot net > GMOD Coordinator (http://gmod.org/) 216-392-3087 > Ontario Institute for Cancer Research > -- ------------------------------------------------------------------------ Scott Cain, Ph. D. scott at scottcain dot net GMOD Coordinator (http://gmod.org/) 216-392-3087 Ontario Institute for Cancer Research |
From: Scott C. <sc...@sc...> - 2019-08-13 18:14:27
|
Hi Chin-Rang, I have a few suggestions: First, if you are only looking to display your RNASeq reads locally, you'll probably find it a lot easier to use the JBrowse desktop application: https://jbrowse.org/docs/jbrowse_desktop.html (download at http://jbrowse.org/blog/2019/07/11/jbrowse-1-16-6.html). It's JBrowse running in an Electron wrapper, so you don't have to deal with a webserver at all. On the other hand if your goal is to get a server running so you can share it with lab mates or the like, you can continue down the road using a webserver. I have no experience trying to set up JBrowse on a Windows machine. While the webserver part should work fine, I would guess that you're likely to run into problems running the processing perl scripts like prepare-refseqs.pl and flatfile-to-json.pl, but that's a step or two away at the moment. To address your immediate problem, please open your javascript console to see if there are any errors that might help us figure out what's going on. Good luck, Scott On Tue, Aug 13, 2019 at 10:07 AM Yang, Chin-Rang (NIH/NHLBI) [E] via Gmod-ajax <gmo...@li...> wrote: > Hi, > > > > I am trying to setup jBrowse on a Apache server at the local Windows 10 > workstation to display my RNA-Seq data. > > > > The Apache server is running OK. Test web page shows “It works”. > > > > I put the Jbrowse folder in the Apache htdocs folder. > > > > When I run > > http://localhost/jbrowse/ > > the web page shows “ Loading …” > > > > I tried the “volvox” example, get tracks.conf, volvox.fa, volvox.fa.fai in > the data folder. > > Then run > > http://localhost/jbrowse/?data=data > > the web page shows “Loading …”, but nothing loaded … > > > > I am wondering if other people have the similar issue. > > Do I need to set jbrowse up in a really working web server, not a local > one? > > > > Please advise. Thank you. > > > > Chin-Rang > > > _______________________________________________ > Gmod-ajax mailing list > Gmo...@li... > https://lists.sourceforge.net/lists/listinfo/gmod-ajax > -- ------------------------------------------------------------------------ Scott Cain, Ph. D. scott at scottcain dot net GMOD Coordinator (http://gmod.org/) 216-392-3087 Ontario Institute for Cancer Research |
From: Yang, Chin-R. (NIH/N. [E] <chi...@ni...> - 2019-08-13 18:00:15
|
Hi Scott, Thank you for your prompt reply. Yes, my plan is to setup a web server for others to access my data in the future. I was thinking to test it out in a local machine first. The error message I found from the Chrome browser is “Failed to load resource: the server responded with a status main.bundle.js:1 of 404 (Not Found)” Thank you very much for your advice. I will talk to our IT people to see how to get an access for a web server as well. Chin-Rang From: Scott Cain <sc...@sc...> Sent: Tuesday, August 13, 2019 1:46 PM To: Yang, Chin-Rang (NIH/NHLBI) [E] <chi...@ni...> Cc: gmo...@li... Subject: Re: [Gmod-ajax] jBrowse set up issue Hi Chin-Rang, I have a few suggestions: First, if you are only looking to display your RNASeq reads locally, you'll probably find it a lot easier to use the JBrowse desktop application: https://jbrowse.org/docs/jbrowse_desktop.html (download at http://jbrowse.org/blog/2019/07/11/jbrowse-1-16-6.html). It's JBrowse running in an Electron wrapper, so you don't have to deal with a webserver at all. On the other hand if your goal is to get a server running so you can share it with lab mates or the like, you can continue down the road using a webserver. I have no experience trying to set up JBrowse on a Windows machine. While the webserver part should work fine, I would guess that you're likely to run into problems running the processing perl scripts like prepare-refseqs.pl<http://prepare-refseqs.pl> and flatfile-to-json.pl<http://flatfile-to-json.pl>, but that's a step or two away at the moment. To address your immediate problem, please open your javascript console to see if there are any errors that might help us figure out what's going on. Good luck, Scott On Tue, Aug 13, 2019 at 10:07 AM Yang, Chin-Rang (NIH/NHLBI) [E] via Gmod-ajax <gmo...@li...<mailto:gmo...@li...>> wrote: Hi, I am trying to setup jBrowse on a Apache server at the local Windows 10 workstation to display my RNA-Seq data. The Apache server is running OK. Test web page shows “It works”. I put the Jbrowse folder in the Apache htdocs folder. When I run http://localhost/jbrowse/ the web page shows “ Loading …” I tried the “volvox” example, get tracks.conf, volvox.fa, volvox.fa.fai in the data folder. Then run http://localhost/jbrowse/?data=data the web page shows “Loading …”, but nothing loaded … I am wondering if other people have the similar issue. Do I need to set jbrowse up in a really working web server, not a local one? Please advise. Thank you. Chin-Rang _______________________________________________ Gmod-ajax mailing list Gmo...@li...<mailto:Gmo...@li...> https://lists.sourceforge.net/lists/listinfo/gmod-ajax -- ------------------------------------------------------------------------ Scott Cain, Ph. D. scott at scottcain dot net GMOD Coordinator (http://gmod.org/) 216-392-3087 Ontario Institute for Cancer Research |
From: Yang, Chin-R. (NIH/N. [E] <chi...@ni...> - 2019-08-13 16:48:01
|
Hi, I am trying to setup jBrowse on a Apache server at the local Windows 10 workstation to display my RNA-Seq data. The Apache server is running OK. Test web page shows "It works". I put the Jbrowse folder in the Apache htdocs folder. When I run http://localhost/jbrowse/ the web page shows " Loading ..." I tried the "volvox" example, get tracks.conf, volvox.fa, volvox.fa.fai in the data folder. Then run http://localhost/jbrowse/?data=data the web page shows "Loading ...", but nothing loaded ... I am wondering if other people have the similar issue. Do I need to set jbrowse up in a really working web server, not a local one? Please advise. Thank you. Chin-Rang |
From: Colin <col...@gm...> - 2019-08-12 15:08:19
|
Hi there, The main thing to note is that generate-names.pl does not accept a specific file as input. By default, generate-names.pl runs on the whole data data directory that you provide to the --out parameter, so you just use bin/generate-names.pl --out /efs/apollo/Nitab-v4.5 This automatically detects the tracks you have loaded in this directory and populates the "names" folder. You can use the -v argument to get verbose. The --tracks argument can accept a list of comma separated track "labels" from the trackList.json/tracks.conf -Colin On Mon, Aug 12, 2019 at 8:39 AM Michał T. Lorenc <m.t...@gm...> wrote: > Hi Scott, > I think, I ran *flatfile-to-json.pl <http://flatfile-to-json.pl>* first > and it generated the following folders: > > > > > > > > > > > > */efs/apollo/Nitab-v4.5$ ls -hlattotal 4.1Gdrwxr-sr-x 10 ubuntu > apollo_users 6.0K Aug 12 04:16 ..-rw-rw-r-- 1 ubuntu apollo_users 541 Aug > 11 23:33 .htaccessdrwxrwsr-x 2 ubuntu apollo_users 14K Aug 11 23:05 > names-rw-rw-r-- 1 ubuntu apollo_users 819 Aug 11 23:04 > trackList.jsondrwxrwsr-x 4 ubuntu apollo_users 6.0K Jul 14 22:23 > tracks-rw-rw-r-- 1 ubuntu apollo_users 0 Jul 14 12:21 > tracks.confdrwxrwsr-x 26 ubuntu apollo_users 6.0K Jul 14 12:21 > seq-rw-rw-r-- 1 ubuntu apollo_users 49M Jul 14 02:39 > Nitab-v4.5_gene_models_Chr_Edwards2017.gff-rw-rw-r-- 1 ubuntu apollo_users > 2.8G Jul 14 02:24 Nitab-v4.5_genome_Chr_Edwards2017.fasta* > > What did I miss? > > Thank you in advance, > > Michal > > On Mon, Aug 12, 2019 at 2:57 PM Scott Cain <sc...@sc...> wrote: > >> Hi Michal, >> >> Did you run flatfile-to-json.pl first? It creates a set of names.txt >> files that are required for generate-names to work on. >> >> Scott >> >> >> On Aug 11, 2019, at 7:20 PM, Michał T. Lorenc <m.t...@gm...> >> wrote: >> >> Hi all, >> I tried to use `generate-names.pl` in the following way: >> >> ``` >> >> *> /efs/apollo/Nitab-v4.5$ perl >> ~/tomcat7-deploy/apollo/jbrowse/bin/prepare-refseqs.pl >> <http://prepare-refseqs.pl> --fasta >> /efs/apollo/Nitab-v4.5_genome_Chr_Edwards2017.fasta --out >> /efs/apollo/Nitab-v4.5> /efs/apollo/Nitab-v4.5$ perl >> ~/tomcat7-deploy/apollo/jbrowse/bin/generate-names.pl >> <http://generate-names.pl> --tracks >> Nitab-v4.5_gene_models_Chr_Edwards2017.gff --out /efs/apollo/Nitab-v4.5* >> ``` >> on the below GFF3 file: >> ``` >> >> >> >> >> >> >> >> >> >> *> cat Nitab-v4.5_gene_models_Chr_Edwards2017.gff...Nt01 maker gene >> 513567 517023 . - . >> ID=Nitab4.5_0002367g0080;Name=Nitab4.5_0002367g0080;Parent=Nitab4.5_0002367Nt01 >> maker mRNA 513567 517023 . - . >> ID=Nitab4.5_0002367g0080.1;Parent=Nitab4.5_0002367g0080;Name=Nitab4.5_0002367g0080.1;_AED=0.01;_eAED=0.01;_QI=61|1|1|1|1|1|2|173|194;Note="Ribosomal >> protein S6%2C plastid/chloroplast%2C Translation elongation factor >> EF1B/ribosomal protein S6%2C Ribosomal protein S6"Nt01 maker exon >> 513567 513979 . - . >> ID=Nitab4.5_0002367g0080.1:exon:2876;Parent=Nitab4.5_0002367g0080.1Nt01 >> maker exon 516618 517023 . - . >> ID=Nitab4.5_0002367g0080.1:exon:2875;Parent=Nitab4.5_0002367g0080.1Nt01 >> maker five_prime_UTR 516963 517023 . - . >> ID=Nitab4.5_0002367g0080.1:five_prime_utr;Parent=Nitab4.5_0002367g0080.1Nt01 >> maker CDS 516618 516962 . - 0 >> ID=Nitab4.5_0002367g0080.1:cds;Parent=Nitab4.5_0002367g0080.1Nt01 maker >> CDS 513740 513979 . - 0 >> ID=Nitab4.5_0002367g0080.1:cds;Parent=Nitab4.5_0002367g0080.1Nt01 maker >> three_prime_UTR 513567 513739 . - . >> ID=Nitab4.5_0002367g0080.1:three_prime_utr;Parent=Nitab4.5_0002367g0080.1* >> ``` >> >> Unfortunately, it did not work. What did I miss? >> >> Thank you in advance. >> >> Michal >> >> _______________________________________________ >> Gmod-ajax mailing list >> Gmo...@li... >> https://lists.sourceforge.net/lists/listinfo/gmod-ajax >> >> _______________________________________________ > Gmod-ajax mailing list > Gmo...@li... > https://lists.sourceforge.net/lists/listinfo/gmod-ajax > |
From: Michał T. L. <m.t...@gm...> - 2019-08-12 12:39:14
|
Hi Scott, I think, I ran *flatfile-to-json.pl <http://flatfile-to-json.pl>* first and it generated the following folders: */efs/apollo/Nitab-v4.5$ ls -hlattotal 4.1Gdrwxr-sr-x 10 ubuntu apollo_users 6.0K Aug 12 04:16 ..-rw-rw-r-- 1 ubuntu apollo_users 541 Aug 11 23:33 .htaccessdrwxrwsr-x 2 ubuntu apollo_users 14K Aug 11 23:05 names-rw-rw-r-- 1 ubuntu apollo_users 819 Aug 11 23:04 trackList.jsondrwxrwsr-x 4 ubuntu apollo_users 6.0K Jul 14 22:23 tracks-rw-rw-r-- 1 ubuntu apollo_users 0 Jul 14 12:21 tracks.confdrwxrwsr-x 26 ubuntu apollo_users 6.0K Jul 14 12:21 seq-rw-rw-r-- 1 ubuntu apollo_users 49M Jul 14 02:39 Nitab-v4.5_gene_models_Chr_Edwards2017.gff-rw-rw-r-- 1 ubuntu apollo_users 2.8G Jul 14 02:24 Nitab-v4.5_genome_Chr_Edwards2017.fasta* What did I miss? Thank you in advance, Michal On Mon, Aug 12, 2019 at 2:57 PM Scott Cain <sc...@sc...> wrote: > Hi Michal, > > Did you run flatfile-to-json.pl first? It creates a set of names.txt > files that are required for generate-names to work on. > > Scott > > > On Aug 11, 2019, at 7:20 PM, Michał T. Lorenc <m.t...@gm...> > wrote: > > Hi all, > I tried to use `generate-names.pl` in the following way: > > ``` > > *> /efs/apollo/Nitab-v4.5$ perl > ~/tomcat7-deploy/apollo/jbrowse/bin/prepare-refseqs.pl > <http://prepare-refseqs.pl> --fasta > /efs/apollo/Nitab-v4.5_genome_Chr_Edwards2017.fasta --out > /efs/apollo/Nitab-v4.5> /efs/apollo/Nitab-v4.5$ perl > ~/tomcat7-deploy/apollo/jbrowse/bin/generate-names.pl > <http://generate-names.pl> --tracks > Nitab-v4.5_gene_models_Chr_Edwards2017.gff --out /efs/apollo/Nitab-v4.5* > ``` > on the below GFF3 file: > ``` > > > > > > > > > > *> cat Nitab-v4.5_gene_models_Chr_Edwards2017.gff...Nt01 maker gene > 513567 517023 . - . > ID=Nitab4.5_0002367g0080;Name=Nitab4.5_0002367g0080;Parent=Nitab4.5_0002367Nt01 > maker mRNA 513567 517023 . - . > ID=Nitab4.5_0002367g0080.1;Parent=Nitab4.5_0002367g0080;Name=Nitab4.5_0002367g0080.1;_AED=0.01;_eAED=0.01;_QI=61|1|1|1|1|1|2|173|194;Note="Ribosomal > protein S6%2C plastid/chloroplast%2C Translation elongation factor > EF1B/ribosomal protein S6%2C Ribosomal protein S6"Nt01 maker exon > 513567 513979 . - . > ID=Nitab4.5_0002367g0080.1:exon:2876;Parent=Nitab4.5_0002367g0080.1Nt01 > maker exon 516618 517023 . - . > ID=Nitab4.5_0002367g0080.1:exon:2875;Parent=Nitab4.5_0002367g0080.1Nt01 > maker five_prime_UTR 516963 517023 . - . > ID=Nitab4.5_0002367g0080.1:five_prime_utr;Parent=Nitab4.5_0002367g0080.1Nt01 > maker CDS 516618 516962 . - 0 > ID=Nitab4.5_0002367g0080.1:cds;Parent=Nitab4.5_0002367g0080.1Nt01 maker > CDS 513740 513979 . - 0 > ID=Nitab4.5_0002367g0080.1:cds;Parent=Nitab4.5_0002367g0080.1Nt01 maker > three_prime_UTR 513567 513739 . - . > ID=Nitab4.5_0002367g0080.1:three_prime_utr;Parent=Nitab4.5_0002367g0080.1* > ``` > > Unfortunately, it did not work. What did I miss? > > Thank you in advance. > > Michal > > _______________________________________________ > Gmod-ajax mailing list > Gmo...@li... > https://lists.sourceforge.net/lists/listinfo/gmod-ajax > > |
From: Scott C. <sc...@sc...> - 2019-08-12 05:26:39
|
Hi Michal, Did you run flatfile-to-json.pl first? It creates a set of names.txt files that are required for generate-names to work on. Scott > On Aug 11, 2019, at 7:20 PM, Michał T. Lorenc <m.t...@gm...> wrote: > > Hi all, > I tried to use `generate-names.pl` in the following way: > > ``` > > /efs/apollo/Nitab-v4.5$ perl ~/tomcat7-deploy/apollo/jbrowse/bin/prepare-refseqs.pl --fasta /efs/apollo/Nitab-v4.5_genome_Chr_Edwards2017.fasta --out /efs/apollo/Nitab-v4.5 > > /efs/apollo/Nitab-v4.5$ perl ~/tomcat7-deploy/apollo/jbrowse/bin/generate-names.pl --tracks Nitab-v4.5_gene_models_Chr_Edwards2017.gff --out /efs/apollo/Nitab-v4.5 > ``` > on the below GFF3 file: > ``` > > cat Nitab-v4.5_gene_models_Chr_Edwards2017.gff > ... > Nt01 maker gene 513567 517023 . - . ID=Nitab4.5_0002367g0080;Name=Nitab4.5_0002367g0080;Parent=Nitab4.5_0002367 > Nt01 maker mRNA 513567 517023 . - . ID=Nitab4.5_0002367g0080.1;Parent=Nitab4.5_0002367g0080;Name=Nitab4.5_0002367g0080.1;_AED=0.01;_eAED=0.01;_QI=61|1|1|1|1|1|2|173|194;Note="Ribosomal protein S6%2C plastid/chloroplast%2C Translation elongation factor EF1B/ribosomal protein S6%2C Ribosomal protein S6" > Nt01 maker exon 513567 513979 . - . ID=Nitab4.5_0002367g0080.1:exon:2876;Parent=Nitab4.5_0002367g0080.1 > Nt01 maker exon 516618 517023 . - . ID=Nitab4.5_0002367g0080.1:exon:2875;Parent=Nitab4.5_0002367g0080.1 > Nt01 maker five_prime_UTR 516963 517023 . - . ID=Nitab4.5_0002367g0080.1:five_prime_utr;Parent=Nitab4.5_0002367g0080.1 > Nt01 maker CDS 516618 516962 . - 0 ID=Nitab4.5_0002367g0080.1:cds;Parent=Nitab4.5_0002367g0080.1 > Nt01 maker CDS 513740 513979 . - 0 ID=Nitab4.5_0002367g0080.1:cds;Parent=Nitab4.5_0002367g0080.1 > Nt01 maker three_prime_UTR 513567 513739 . - . ID=Nitab4.5_0002367g0080.1:three_prime_utr;Parent=Nitab4.5_0002367g0080.1 > ``` > > Unfortunately, it did not work. What did I miss? > > Thank you in advance. > > Michal > _______________________________________________ > Gmod-ajax mailing list > Gmo...@li... > https://lists.sourceforge.net/lists/listinfo/gmod-ajax |
From: Michał T. L. <m.t...@gm...> - 2019-08-12 02:20:22
|
Hi all, I tried to use `generate-names.pl` in the following way: ``` *> /efs/apollo/Nitab-v4.5$ perl ~/tomcat7-deploy/apollo/jbrowse/bin/prepare-refseqs.pl <http://prepare-refseqs.pl> --fasta /efs/apollo/Nitab-v4.5_genome_Chr_Edwards2017.fasta --out /efs/apollo/Nitab-v4.5> /efs/apollo/Nitab-v4.5$ perl ~/tomcat7-deploy/apollo/jbrowse/bin/generate-names.pl <http://generate-names.pl> --tracks Nitab-v4.5_gene_models_Chr_Edwards2017.gff --out /efs/apollo/Nitab-v4.5* ``` on the below GFF3 file: ``` *> cat Nitab-v4.5_gene_models_Chr_Edwards2017.gff...Nt01 maker gene 513567 517023 . - . ID=Nitab4.5_0002367g0080;Name=Nitab4.5_0002367g0080;Parent=Nitab4.5_0002367Nt01 maker mRNA 513567 517023 . - . ID=Nitab4.5_0002367g0080.1;Parent=Nitab4.5_0002367g0080;Name=Nitab4.5_0002367g0080.1;_AED=0.01;_eAED=0.01;_QI=61|1|1|1|1|1|2|173|194;Note="Ribosomal protein S6%2C plastid/chloroplast%2C Translation elongation factor EF1B/ribosomal protein S6%2C Ribosomal protein S6"Nt01 maker exon 513567 513979 . - . ID=Nitab4.5_0002367g0080.1:exon:2876;Parent=Nitab4.5_0002367g0080.1Nt01 maker exon 516618 517023 . - . ID=Nitab4.5_0002367g0080.1:exon:2875;Parent=Nitab4.5_0002367g0080.1Nt01 maker five_prime_UTR 516963 517023 . - . ID=Nitab4.5_0002367g0080.1:five_prime_utr;Parent=Nitab4.5_0002367g0080.1Nt01 maker CDS 516618 516962 . - 0 ID=Nitab4.5_0002367g0080.1:cds;Parent=Nitab4.5_0002367g0080.1Nt01 maker CDS 513740 513979 . - 0 ID=Nitab4.5_0002367g0080.1:cds;Parent=Nitab4.5_0002367g0080.1Nt01 maker three_prime_UTR 513567 513739 . - . ID=Nitab4.5_0002367g0080.1:three_prime_utr;Parent=Nitab4.5_0002367g0080.1* ``` Unfortunately, it did not work. What did I miss? Thank you in advance. Michal |
From: Colin <col...@gm...> - 2019-08-09 13:10:04
|
Hi there, There are several options for how the action can occur 1. Configuring left-click 2. Configuring right-click menu 3. Customizing the View details popup These are all documented in the jbrowse docs https://jbrowse.org/docs/mouse_configs.html The callback can contain such things like this function(feature) { return 'http://google.com/?q='+feature.get('Dbxref') } That will generate a google link for a Dbxref. Note the Dbxref in full is *TIGRFAM*:TIGR00756 not just the bolded part It will just take a little javascript if you want to add conditional statements or parse the string or something like this -Colin On Thu, Aug 8, 2019 at 10:50 PM Michał T. Lorenc <m.t...@gm...> wrote: > Hi, > I have below a subset of a GFF3 file which contains different DbXrefs, > InterProScan accession numbers and GO terms. However, some lines of the > GFF3 are missing InterProScan accession numbers and GO terms. However, all > lines contain DbXrefs. Here (https://www.uniprot.org/docs/dbxref) is a > list of all possible dbxrefs. > > How is possible to allow the user to click in JBrowse on a feature and get > options to be redirected to DbXrefs, InterProScan and ontogy? > > - NbV1Ch09 interpo domain 71124892 71125030 . . . Name=RNase_HI_RT_Ty1; > *Dbxref=CDD*:cd09272;Note=CDD > - NbV1Ch14 interpo domain 93274595 93274797 . . . Name=Ribonuclease H > superfamily;*Dbxref=Gene3D*:G3DSA:3.30.420.10;Note=Gene3D;_Accession= > *IPR036397*;Ontology_term=*GO:0003676* > - NbV1Ch16 interpo domain 66731787 66732421 . . . > Name=Deoxyxylulose-5-phosphate synthase;*Dbxref=Hamap* > :MF_00315;Note=Hamap;_Accession=*IPR005477*;Ontology_term= > *GO:0008661,GO:0016114* > - NbV1Ch09 interpo domain 71124160 71124256 . . . Name=consensus > disorder prediction;*Dbxref=MobiDBLite*:mobidb-lite;Note=MobiDBLite > - NbV1Ch01 interpo domain 175378467 175379194 . . . Name=AP-1/2/4 > complex subunit beta;*Dbxref=PIRSF*:PIRSF002291;Note=PIRSF;_Accession= > *IPR016342*;Ontology_term=*GO:0015031,GO:0030276* > - NbV1Ch07 interpo domain 107909842 107909860 . . . Name=Recoverin > family signature;*Dbxref=PRINTS*:PR00450;Note=PRINTS > - NbV1Ch09 interpo domain 71123771 71123905 . . . Name=gag-polypeptide > of LTR copia-type;*Dbxref=Pfam*:PF14223;Note=Pfam > - NbV1Ch03 interpo domain 33983707 33983721 . . . Name=Trp-Asp (WD) > repeats signature.;*Dbxref=ProSitePatterns* > :PS00678;Note=ProSitePatterns;_Accession=*IPR019775* > - NbV1Ch09 interpo domain 71124317 71124413 . . . Name=Integrase, > catalytic core;*Dbxref=ProSiteProfiles* > :PS50994;Note=ProSiteProfiles;_Accession=*IPR001584*;Ontology_term= > *GO:0015074* > - NbV1Ch17 interpo domain 30522620 30522886 . . . Name=C1.5: HAD, > Beta-PGM, Phosphatase Like;*Dbxref=SFLD*:SFLDG01129;Note=SFLD > - NbV1Ch03 interpo domain 33983723 33983761 . . . Name=WD40 repeat; > *Dbxref=SMART*:SM00320;Note=SMART;_Accession=*IPR001680*;Ontology_term= > *GO:0005515* > - NbV1Ch09 interpo domain 71124311 71124414 . . . Name=Ribonuclease > H-like superfamily;*Dbxref=SUPERFAMILY* > :SSF53098;Note=SUPERFAMILY;_Accession=*IPR012337* > - NbV1Ch01 interpo domain 157928056 157928088 . . . Name=PPR: > pentatricopeptide repeat domain;*Dbxref=TIGRFAM* > :TIGR00756;Note=TIGRFAM;_Accession=*IPR002885* > > Thank you in advance, > > Michal > _______________________________________________ > Gmod-ajax mailing list > Gmo...@li... > https://lists.sourceforge.net/lists/listinfo/gmod-ajax > |
From: Michał T. L. <m.t...@gm...> - 2019-08-09 02:49:57
|
Hi, I have below a subset of a GFF3 file which contains different DbXrefs, InterProScan accession numbers and GO terms. However, some lines of the GFF3 are missing InterProScan accession numbers and GO terms. However, all lines contain DbXrefs. Here (https://www.uniprot.org/docs/dbxref) is a list of all possible dbxrefs. How is possible to allow the user to click in JBrowse on a feature and get options to be redirected to DbXrefs, InterProScan and ontogy? - NbV1Ch09 interpo domain 71124892 71125030 . . . Name=RNase_HI_RT_Ty1; *Dbxref=CDD*:cd09272;Note=CDD - NbV1Ch14 interpo domain 93274595 93274797 . . . Name=Ribonuclease H superfamily;*Dbxref=Gene3D*:G3DSA:3.30.420.10;Note=Gene3D;_Accession= *IPR036397*;Ontology_term=*GO:0003676* - NbV1Ch16 interpo domain 66731787 66732421 . . . Name=Deoxyxylulose-5-phosphate synthase;*Dbxref=Hamap* :MF_00315;Note=Hamap;_Accession=*IPR005477*;Ontology_term= *GO:0008661,GO:0016114* - NbV1Ch09 interpo domain 71124160 71124256 . . . Name=consensus disorder prediction;*Dbxref=MobiDBLite*:mobidb-lite;Note=MobiDBLite - NbV1Ch01 interpo domain 175378467 175379194 . . . Name=AP-1/2/4 complex subunit beta;*Dbxref=PIRSF*:PIRSF002291;Note=PIRSF;_Accession= *IPR016342*;Ontology_term=*GO:0015031,GO:0030276* - NbV1Ch07 interpo domain 107909842 107909860 . . . Name=Recoverin family signature;*Dbxref=PRINTS*:PR00450;Note=PRINTS - NbV1Ch09 interpo domain 71123771 71123905 . . . Name=gag-polypeptide of LTR copia-type;*Dbxref=Pfam*:PF14223;Note=Pfam - NbV1Ch03 interpo domain 33983707 33983721 . . . Name=Trp-Asp (WD) repeats signature.;*Dbxref=ProSitePatterns* :PS00678;Note=ProSitePatterns;_Accession=*IPR019775* - NbV1Ch09 interpo domain 71124317 71124413 . . . Name=Integrase, catalytic core;*Dbxref=ProSiteProfiles* :PS50994;Note=ProSiteProfiles;_Accession=*IPR001584*;Ontology_term= *GO:0015074* - NbV1Ch17 interpo domain 30522620 30522886 . . . Name=C1.5: HAD, Beta-PGM, Phosphatase Like;*Dbxref=SFLD*:SFLDG01129;Note=SFLD - NbV1Ch03 interpo domain 33983723 33983761 . . . Name=WD40 repeat; *Dbxref=SMART*:SM00320;Note=SMART;_Accession=*IPR001680*;Ontology_term= *GO:0005515* - NbV1Ch09 interpo domain 71124311 71124414 . . . Name=Ribonuclease H-like superfamily;*Dbxref=SUPERFAMILY* :SSF53098;Note=SUPERFAMILY;_Accession=*IPR012337* - NbV1Ch01 interpo domain 157928056 157928088 . . . Name=PPR: pentatricopeptide repeat domain;*Dbxref=TIGRFAM* :TIGR00756;Note=TIGRFAM;_Accession=*IPR002885* Thank you in advance, Michal |
From: Scott C. <sc...@sc...> - 2019-08-08 17:51:45
|
Hi Michał, Can you be a little more specific when you say it doesn't work for BED data? What doesn't work, hiding the labels or displaying the data at all or something else? Thanks, Scott On Wed, Aug 7, 2019 at 6:45 PM Michał T. Lorenc <m.t...@gm...> wrote: > Hi Scott, > Thank you it worked for GFF3 files but it does not work for the below BED > file: > > > > > > > > > > > > > > > * { "category" : "iii. ChIP-seq", "compress" : 1, > "key" : "QldK9.bed", "label" : "QldK9.bed", > "storeClass" : "JBrowse/Store/SeqFeature/NCList", "style" : { > "className" : "feature", "label" : false }, > "trackType" : "CanvasFeatures", "type" : "CanvasFeatures", > "urlTemplate" : "tracks/QldK9.bed/{refseq}/trackData.jsonz" },* > > What did I miss? > > Thank you in advance, > > Michal > > > > > On Wed, Jul 31, 2019 at 3:52 PM Scott Cain <sc...@sc...> wrote: > >> Hi Michal, >> >> Yes, you can suppress the feature labels by adding "label" : false to the >> style section of the trackList.json config section. >> >> For adding a link for a feature, it is moderately more complicated and >> depends on where you want it to link to. This section of the docs will get >> you started: >> >> >> http://jbrowse.org/docs/mouse_configs.html#customizing-left-click-behavior-1 >> >> but you might need a javascript callback to get it working. If it's not >> obvious, please respond and give a description of what you'd like the link >> to do and we can give you more help. >> >> Scott >> >> >> On Tue, Jul 30, 2019 at 10:03 PM Michał T. Lorenc <m.t...@gm...> >> wrote: >> >>> Hi, >>> I have a GFF3 file with SNPs (please see below) and I loaded it into >>> Jbrowse/Apollo with *flatfile-to-json.pl <http://flatfile-to-json.pl> >>> --gff /efs/apollo/snps.fasta.m_snp.gff3 --compress --trackLabel "SNPs" >>> --out /efs/apollo/ * . >>> >>> >>> >>> *##gff-version 3NbV1Ch01 SGSautoSNP SNP 1245 1245 >>> . . . >>> Name=NbV1ChF00000000000000000001;ID=NbV1ChF00000000000000000001;Contig >>> name=NbV1Ch01;SNP score=5;SNP pos. on scaffold=1245;Genotype >>> L=26*T;Genotype Q=5*C;Changes=C/T* >>> *NbV1Ch01 SGSautoSNP SNP 1303 1303 . . >>> . >>> Name=NbV1ChF00000000000000000002;ID=NbV1ChF00000000000000000002;Contig >>> name=NbV1Ch01;SNP score=4;SNP pos. on scaffold=1303;Genotype >>> L=18*T;Genotype Q=4*A;Changes=A/T* >>> >>> [image: image.png] >>> >>> How is it possible to hide the SNP description and only show it when the >>> user mouse over or click on the SNP? Additionally, when the SNP description >>> appears would it be possible to add a click event to be redirected to a new >>> page? >>> >>> Thank you in advance, >>> >>> Best wishes, >>> >>> Michal >>> >>> >>> _______________________________________________ >>> Gmod-ajax mailing list >>> Gmo...@li... >>> https://lists.sourceforge.net/lists/listinfo/gmod-ajax >>> >> >> >> -- >> ------------------------------------------------------------------------ >> Scott Cain, Ph. D. scott at scottcain >> dot net >> GMOD Coordinator (http://gmod.org/) 216-392-3087 >> Ontario Institute for Cancer Research >> > -- ------------------------------------------------------------------------ Scott Cain, Ph. D. scott at scottcain dot net GMOD Coordinator (http://gmod.org/) 216-392-3087 Ontario Institute for Cancer Research |
From: Michał T. L. <m.t...@gm...> - 2019-08-08 01:45:14
|
Hi Scott, Thank you it worked for GFF3 files but it does not work for the below BED file: * { "category" : "iii. ChIP-seq", "compress" : 1, "key" : "QldK9.bed", "label" : "QldK9.bed", "storeClass" : "JBrowse/Store/SeqFeature/NCList", "style" : { "className" : "feature", "label" : false }, "trackType" : "CanvasFeatures", "type" : "CanvasFeatures", "urlTemplate" : "tracks/QldK9.bed/{refseq}/trackData.jsonz" },* What did I miss? Thank you in advance, Michal On Wed, Jul 31, 2019 at 3:52 PM Scott Cain <sc...@sc...> wrote: > Hi Michal, > > Yes, you can suppress the feature labels by adding "label" : false to the > style section of the trackList.json config section. > > For adding a link for a feature, it is moderately more complicated and > depends on where you want it to link to. This section of the docs will get > you started: > > > http://jbrowse.org/docs/mouse_configs.html#customizing-left-click-behavior-1 > > but you might need a javascript callback to get it working. If it's not > obvious, please respond and give a description of what you'd like the link > to do and we can give you more help. > > Scott > > > On Tue, Jul 30, 2019 at 10:03 PM Michał T. Lorenc <m.t...@gm...> > wrote: > >> Hi, >> I have a GFF3 file with SNPs (please see below) and I loaded it into >> Jbrowse/Apollo with *flatfile-to-json.pl <http://flatfile-to-json.pl> >> --gff /efs/apollo/snps.fasta.m_snp.gff3 --compress --trackLabel "SNPs" >> --out /efs/apollo/ * . >> >> >> >> *##gff-version 3NbV1Ch01 SGSautoSNP SNP 1245 1245 . >> . . >> Name=NbV1ChF00000000000000000001;ID=NbV1ChF00000000000000000001;Contig >> name=NbV1Ch01;SNP score=5;SNP pos. on scaffold=1245;Genotype >> L=26*T;Genotype Q=5*C;Changes=C/T* >> *NbV1Ch01 SGSautoSNP SNP 1303 1303 . . >> . >> Name=NbV1ChF00000000000000000002;ID=NbV1ChF00000000000000000002;Contig >> name=NbV1Ch01;SNP score=4;SNP pos. on scaffold=1303;Genotype >> L=18*T;Genotype Q=4*A;Changes=A/T* >> >> [image: image.png] >> >> How is it possible to hide the SNP description and only show it when the >> user mouse over or click on the SNP? Additionally, when the SNP description >> appears would it be possible to add a click event to be redirected to a new >> page? >> >> Thank you in advance, >> >> Best wishes, >> >> Michal >> >> >> _______________________________________________ >> Gmod-ajax mailing list >> Gmo...@li... >> https://lists.sourceforge.net/lists/listinfo/gmod-ajax >> > > > -- > ------------------------------------------------------------------------ > Scott Cain, Ph. D. scott at scottcain > dot net > GMOD Coordinator (http://gmod.org/) 216-392-3087 > Ontario Institute for Cancer Research > |
From: Scott C. <sc...@sc...> - 2019-07-31 06:21:13
|
Hi Michal, Yes, you can suppress the feature labels by adding "label" : false to the style section of the trackList.json config section. For adding a link for a feature, it is moderately more complicated and depends on where you want it to link to. This section of the docs will get you started: http://jbrowse.org/docs/mouse_configs.html#customizing-left-click-behavior-1 but you might need a javascript callback to get it working. If it's not obvious, please respond and give a description of what you'd like the link to do and we can give you more help. Scott On Tue, Jul 30, 2019 at 10:03 PM Michał T. Lorenc <m.t...@gm...> wrote: > Hi, > I have a GFF3 file with SNPs (please see below) and I loaded it into > Jbrowse/Apollo with *flatfile-to-json.pl <http://flatfile-to-json.pl> > --gff /efs/apollo/snps.fasta.m_snp.gff3 --compress --trackLabel "SNPs" > --out /efs/apollo/ * . > > > > *##gff-version 3NbV1Ch01 SGSautoSNP SNP 1245 1245 . > . . > Name=NbV1ChF00000000000000000001;ID=NbV1ChF00000000000000000001;Contig > name=NbV1Ch01;SNP score=5;SNP pos. on scaffold=1245;Genotype > L=26*T;Genotype Q=5*C;Changes=C/T* > *NbV1Ch01 SGSautoSNP SNP 1303 1303 . . . > > Name=NbV1ChF00000000000000000002;ID=NbV1ChF00000000000000000002;Contig > name=NbV1Ch01;SNP score=4;SNP pos. on scaffold=1303;Genotype > L=18*T;Genotype Q=4*A;Changes=A/T* > > [image: image.png] > > How is it possible to hide the SNP description and only show it when the > user mouse over or click on the SNP? Additionally, when the SNP description > appears would it be possible to add a click event to be redirected to a new > page? > > Thank you in advance, > > Best wishes, > > Michal > > > _______________________________________________ > Gmod-ajax mailing list > Gmo...@li... > https://lists.sourceforge.net/lists/listinfo/gmod-ajax > -- ------------------------------------------------------------------------ Scott Cain, Ph. D. scott at scottcain dot net GMOD Coordinator (http://gmod.org/) 216-392-3087 Ontario Institute for Cancer Research |
From: Michał T. L. <m.t...@gm...> - 2019-07-31 05:03:33
|
Hi, I have a GFF3 file with SNPs (please see below) and I loaded it into Jbrowse/Apollo with *flatfile-to-json.pl <http://flatfile-to-json.pl> --gff /efs/apollo/snps.fasta.m_snp.gff3 --compress --trackLabel "SNPs" --out /efs/apollo/ * . *##gff-version 3NbV1Ch01 SGSautoSNP SNP 1245 1245 . . . Name=NbV1ChF00000000000000000001;ID=NbV1ChF00000000000000000001;Contig name=NbV1Ch01;SNP score=5;SNP pos. on scaffold=1245;Genotype L=26*T;Genotype Q=5*C;Changes=C/T* *NbV1Ch01 SGSautoSNP SNP 1303 1303 . . . Name=NbV1ChF00000000000000000002;ID=NbV1ChF00000000000000000002;Contig name=NbV1Ch01;SNP score=4;SNP pos. on scaffold=1303;Genotype L=18*T;Genotype Q=4*A;Changes=A/T* [image: image.png] How is it possible to hide the SNP description and only show it when the user mouse over or click on the SNP? Additionally, when the SNP description appears would it be possible to add a click event to be redirected to a new page? Thank you in advance, Best wishes, Michal |
From: Colin <col...@gm...> - 2019-07-29 18:16:23
|
Hi there, The JBrowse desktop installations don't require the setup.sh script to be run generally If you are intending to put your JBrowse instance on the web, you should use the normal JBrowse-1.16.5.zip or JBrowse-1.16.5-dev.zip Linking BLAST results to jbrowse can be done using different ways, one way I have done is using sequenceserver and that doesn't require any special build of jbrowse. It's not super easy to configure but not too bad either https://jbrowse.org/docs/faq.html#how-can-i-link-blast-results-to-jbrowse -Colin On Mon, Jul 29, 2019 at 1:58 PM 一恆 <114...@qq...> wrote: > Hello sir > My name is Liangye Xu. I'm Zhiqiang Xia's student. I'm working > in Chinese Academy of Tropical Agricultural Sciences(CATAS). I want to use > the JBrowser to show our data of cassava. But in the process of > installation, I encountered a problem that could not be solved. This is my > setup.log file. Could you tell me how to solve this problem, thank you very > much. > If I link BLAST results to JBrowse. Do I must setup the development > version of JBrowser? > _______________________________________________ > Gmod-ajax mailing list > Gmo...@li... > https://lists.sourceforge.net/lists/listinfo/gmod-ajax > |
From: <114...@qq...> - 2019-07-29 02:20:42
|
Hello sir My name is Liangye Xu. I'm Zhiqiang Xia's student. I'm working in Chinese Academy of Tropical Agricultural Sciences(CATAS). I want to use the JBrowser to show our data of cassava. But in the process of installation, I encountered a problem that could not be solved. This is my setup.log file. Could you tell me how to solve this problem, thank you very much. If I link BLAST results to JBrowse. Do I must setup the development version of JBrowser? |
From: Michał T. L. <m.t...@gm...> - 2019-07-28 00:11:17
|
Hi, I tried to install ElasticSearch plugin ( https://github.com/elsiklab/jbrowse_elasticsearch ) into Apollo. With the following steps: 1. git clone https://github.com/GMOD/Apollo.git 2. cd Apollo 3. I added ElasticSearch to `cat docker-files/docker-apollo-config.groovy` *```jbrowse { git { url = "https://github.com/GMOD/jbrowse" branch = "1.16.4-release" } plugins { ... ElasticSearch{ git = 'https://github.com/elsiklab/jbrowse_elasticsearch' branch = 'master' alwaysRecheck = "true" alwaysPull = "true" } }}* ``` Next, I executed *```docker build -t apollo .docker run -it -v "$(pwd)"/mydata:/data -p 8888:8080 -p 8080:3000 apollo```* - http://localhost:8080/ does not work for elasticsearch - http://localhost:8888/ only Apollo works without elasticsearch What did I miss? Thank you in advance, Michal |
From: Scott C. <sc...@sc...> - 2019-07-25 21:04:01
|
Hi Michal, The thing that is needed is a tool to map your InterProScan results on to chromosomes. Off hand I don’t know of a generic one but one might exist. I wonder if there is a Galaxy workflow for it. Anyone on the list know? Scott > On Jul 25, 2019, at 7:53 AM, Michał T. Lorenc <m.t...@gm...> wrote: > > Hi > I ran InterProScan and I got GFF3, TSV and XML. It appears that the InterProScan's GFF3 file does not contain any chromosome names: > > ``` > ##gff-version 3 > ##feature-ontology http://song.cvs.sourceforge.net/viewvc/song/ontology/sofa.obo?revision=1.269 > ##interproscan-version 5.36-75.0 > ##sequence-region g109343.t1 1 1358 > g109343.t1 . polypeptide 1 1358 . + . ID=g109343.t1;md5=3e908dc966fefe367e64dc9d98b0d3ab > g109343.t1 ProSiteProfiles protein_match 628 724 18.261 + . date=20-07-2019;Target=g109343.t1 628 724;Ontology_term="GO:0015074";ID=match$1_628_724;signature_desc=Integrase c > atalytic domain profile.;Name=PS50994;status=T;Dbxref="InterPro:IPR001584" > g109343.t1 SUPERFAMILY protein_match 586 624 4.19E-5 + . date=20-07-2019;Target=g109343.t1 586 624;Ontology_term="GO:0003676","GO:0008270";ID=match$2_586_624;Name=SSF57756 > ;status=T;Dbxref="InterPro:IPR036875" > g109343.t1 SUPERFAMILY protein_match 622 725 4.93E-29 + . date=20-07-2019;Target=g109343.t1 622 725;ID=match$3_622_725;Name=SSF53098;status=T;Dbxref="InterPro:IPR01 > 2337" > g109343.t1 ProSiteProfiles protein_match 278 294 9.636 + . date=20-07-2019;Target=g109343.t1 278 294;Ontology_term="GO:0003676","GO:0008270";ID=match$4_278_294;signature_des > c=Zinc finger CCHC-type profile.;Name=PS50158;status=T;Dbxref="InterPro:IPR001878" > g109343.t1 SMART protein_match 600 616 0.36 + . date=20-07-2019;Target=g109343.t1 600 616;Ontology_term="GO:0003676","GO:0008270";ID=match$5_600_616;Name=SM00343;status=T > ;Dbxref="InterPro:IPR001878" > g109343.t1 SMART protein_match 278 294 6.3E-4 + . date=20-07-2019;Target=g109343.t1 278 294;Ontology_term="GO:0003676","GO:0008270";ID=match$5_278_294;Name=SM00343;status=T > ;Dbxref="InterPro:IPR001878" > g109343.t1 Pfam protein_match 82 216 1.2E-24 + . date=20-07-2019;Target=g109343.t1 82 216;ID=match$6_82_216;signature_desc=gag-polypeptide of LTR copia-type;Name=PF14223;s > tatus=T > ``` > Is there a solution how to load InterProScan results as a track into JBrowse? > > Thank you in advance, > > Michal > _______________________________________________ > Gmod-ajax mailing list > Gmo...@li... > https://lists.sourceforge.net/lists/listinfo/gmod-ajax |
From: Michał T. L. <m.t...@gm...> - 2019-07-25 14:53:42
|
Hi I ran InterProScan and I got GFF3, TSV and XML. It appears that the InterProScan's GFF3 file does not contain any chromosome names: ``` *##gff-version 3##feature-ontology http://song.cvs.sourceforge.net/viewvc/song/ontology/sofa.obo?revision=1.269 <http://song.cvs.sourceforge.net/viewvc/song/ontology/sofa.obo?revision=1.269>##interproscan-version 5.36-75.0##sequence-region g109343.t1 1 1358g109343.t1 . polypeptide 1 1358 . + . ID=g109343.t1;md5=3e908dc966fefe367e64dc9d98b0d3abg109343.t1 ProSiteProfiles protein_match 628 724 18.261 + . date=20-07-2019;Target=g109343.t1 628 724;Ontology_term="GO:0015074";ID=match$1_628_724;signature_desc=Integrase catalytic domain profile.;Name=PS50994;status=T;Dbxref="InterPro:IPR001584"g109343.t1 SUPERFAMILY protein_match 586 624 4.19E-5 + . date=20-07-2019;Target=g109343.t1 586 624;Ontology_term="GO:0003676","GO:0008270";ID=match$2_586_624;Name=SSF57756;status=T;Dbxref="InterPro:IPR036875"g109343.t1 SUPERFAMILY protein_match 622 725 4.93E-29 + . date=20-07-2019;Target=g109343.t1 622 725;ID=match$3_622_725;Name=SSF53098;status=T;Dbxref="InterPro:IPR012337"g109343.t1 ProSiteProfiles protein_match 278 294 9.636 + . date=20-07-2019;Target=g109343.t1 278 294;Ontology_term="GO:0003676","GO:0008270";ID=match$4_278_294;signature_desc=Zinc finger CCHC-type profile.;Name=PS50158;status=T;Dbxref="InterPro:IPR001878"g109343.t1 SMART protein_match 600 616 0.36 + . date=20-07-2019;Target=g109343.t1 600 616;Ontology_term="GO:0003676","GO:0008270";ID=match$5_600_616;Name=SM00343;status=T;Dbxref="InterPro:IPR001878"g109343.t1 SMART protein_match 278 294 6.3E-4 + . date=20-07-2019;Target=g109343.t1 278 294;Ontology_term="GO:0003676","GO:0008270";ID=match$5_278_294;Name=SM00343;status=T;Dbxref="InterPro:IPR001878"g109343.t1 Pfam protein_match 82 216 1.2E-24 + . date=20-07-2019;Target=g109343.t1 82 216;ID=match$6_82_216;signature_desc=gag-polypeptide of LTR copia-type;Name=PF14223;status=T* ``` Is there a solution how to load InterProScan results as a track into JBrowse? Thank you in advance, Michal |
From: Garrett S. <ste...@gm...> - 2019-07-24 21:36:18
|
Hello Yael, I'm not sure the coloring works with BED files imported using flatfile-to-json, but it should work if you index the BED file and use it directly. So, for example, if you had a file test.bed in your data/ directory, you could do `bgzip test.bed` and then `tabix test.bed.gz` (bgzip and tabix are from htslib <https://www.htslib.org/>). Then add the following lines to data/tracks.conf: [tracks.test1] urlTemplate=test.bed.gz This should add the new track called "test1" that has the coloring you want. You can read more about indexed file formats here: http://jbrowse.org/docs/tutorial.html Best, Garrett On Wed, Jul 24, 2019 at 12:50 PM Yael Altuvia <ya...@gm...> wrote: > > Hi, > > We are now trying to switch from using the UCSC browser to JBrowse > (version 2.1) for some specific organisms > We use firefox browser in a unix environment > > We found JBrowse to be very useful for our purpose. > > We are now trying to to upload BED file with multiple coloring > > example input lines of my BED file > > chr 3635789 3635819 NB500937:176:HNWM7AFXX:1:11207:14092:2893_5p 0 > + 3635789 3635819 255,0,0 > chr 2364664 2364721 NB500937:176:HNWM7AFXX:1:11207:14092:2893_3p 0 > + 2364664 2364721 51,102,255 > chr 1798195 1798236 NB500937:176:HNWM7AFXX:3:11511:24854:8176_5p 0 > - 1798195 1798236 255,0,0 > chr 2364637 2364676 NB500937:176:HNWM7AFXX:3:11511:24854:8176_3p 0 > + 2364637 2364676 51,102,255 > chr 1047431 1047504 NB500937:176:HNWM7AFXX:2:21101:21711:9398_5p 0 > - 1047431 1047504 255,0,0 > chr 2364637 2364675 NB500937:176:HNWM7AFXX:2:21101:21711:9398_3p 0 > + 2364637 2364675 51,102,255 > chr 1204394 1204438 NB500937:176:HNWM7AFXX:1:11310:23077:3067_5p 0 > - 1204394 1204438 255,0,0 > chr 2364664 2364721 NB500937:176:HNWM7AFXX:1:11310:23077:3067_3p 0 > + 2364664 2364721 51,102,255 > > We use the following rflatfile-to-json.pl run command: > > /home/www/html/millstone/jbrowse/bin/flatfile-to-json.pl --bed temp.test > --out <OUT-DIR> --trackType CanvasFeatures --clientConfig '{"showLabels": > false,"height": 1,"marginBottom": 0.5,"strandArrow": false}' --config > '{"ItemRgb": true}' --tracklabel temptest1 > > The generated track is loaded and presented, however all elements are > colored golenrod and not according to the BED specifications > > We tried to add "glyph": "BED" > but then the track is not loaded properly - it is showing "loading" for > long time even for the smallest file with only 10 lines > > These are the attributed seen with the edit option of the track: > { > "maxFeatureSizeForUnderlyingRefSeq": 250000, > "maxFeatureScreenDensity": 3, > "maxFeatureGlyphExpansion": 500, > "maxHeight": 600, > "histograms": { > "description": "feature density", > "min": 0, > "height": 100, > "color": "goldenrod", > "clip_marker_color": "red" > }, > "style": { > "_defaultHistScale": 4, > "_defaultLabelScale": 30, > "_defaultDescriptionScale": 120, > "showLabels": false, > "showTooltips": true, > "className": "feature", > "height": 1, > "marginBottom": 0.5, > "strandArrow": false > }, > "displayMode": "normal", > "events": {}, > "menuTemplate": [ > { > "label": "View details", > "title": "{type} {name}", > "action": "contentDialog", > "iconClass": "dijitIconTask" > }, > { > "iconClass": "dijitIconConnector" > }, > { > "iconClass": "dijitIconFilter" > } > ], > "type": "JBrowse/View/Track/CanvasFeatures", > "storeClass": "JBrowse/Store/SeqFeature/NCList", > "label": "temptest1", > "urlTemplate": "tracks/temptest1/{refseq}/trackData.json", > "key": "temptest1", > "trackType": "CanvasFeatures", > "ItemRgb": true, > "compress": 0, > "baseUrl": " > http://menash.md.huji.ac.il/millstone/jbrowse/data/dir-for-gianluca//", > "index": 9, > "metadata": {} > } > > > What are we doing wrong? > > Also we would also like to know if it is possible to save a session e.g. > if tracks were manually edited can they all be saved with the new > configurations? > > Thanks, > Yael > > _______________________________________________ > Gmod-ajax mailing list > Gmo...@li... > https://lists.sourceforge.net/lists/listinfo/gmod-ajax > |
From: Yael A. <ya...@gm...> - 2019-07-24 08:24:59
|
Hi, We are now trying to switch from using the UCSC browser to JBrowse (version 2.1) for some specific organisms We use firefox browser in a unix environment We found JBrowse to be very useful for our purpose. We are now trying to to upload BED file with multiple coloring example input lines of my BED file chr 3635789 3635819 NB500937:176:HNWM7AFXX:1:11207:14092:2893_5p 0 + 3635789 3635819 255,0,0 chr 2364664 2364721 NB500937:176:HNWM7AFXX:1:11207:14092:2893_3p 0 + 2364664 2364721 51,102,255 chr 1798195 1798236 NB500937:176:HNWM7AFXX:3:11511:24854:8176_5p 0 - 1798195 1798236 255,0,0 chr 2364637 2364676 NB500937:176:HNWM7AFXX:3:11511:24854:8176_3p 0 + 2364637 2364676 51,102,255 chr 1047431 1047504 NB500937:176:HNWM7AFXX:2:21101:21711:9398_5p 0 - 1047431 1047504 255,0,0 chr 2364637 2364675 NB500937:176:HNWM7AFXX:2:21101:21711:9398_3p 0 + 2364637 2364675 51,102,255 chr 1204394 1204438 NB500937:176:HNWM7AFXX:1:11310:23077:3067_5p 0 - 1204394 1204438 255,0,0 chr 2364664 2364721 NB500937:176:HNWM7AFXX:1:11310:23077:3067_3p 0 + 2364664 2364721 51,102,255 We use the following rflatfile-to-json.pl run command: /home/www/html/millstone/jbrowse/bin/flatfile-to-json.pl --bed temp.test --out <OUT-DIR> --trackType CanvasFeatures --clientConfig '{"showLabels": false,"height": 1,"marginBottom": 0.5,"strandArrow": false}' --config '{"ItemRgb": true}' --tracklabel temptest1 The generated track is loaded and presented, however all elements are colored golenrod and not according to the BED specifications We tried to add "glyph": "BED" but then the track is not loaded properly - it is showing "loading" for long time even for the smallest file with only 10 lines These are the attributed seen with the edit option of the track: { "maxFeatureSizeForUnderlyingRefSeq": 250000, "maxFeatureScreenDensity": 3, "maxFeatureGlyphExpansion": 500, "maxHeight": 600, "histograms": { "description": "feature density", "min": 0, "height": 100, "color": "goldenrod", "clip_marker_color": "red" }, "style": { "_defaultHistScale": 4, "_defaultLabelScale": 30, "_defaultDescriptionScale": 120, "showLabels": false, "showTooltips": true, "className": "feature", "height": 1, "marginBottom": 0.5, "strandArrow": false }, "displayMode": "normal", "events": {}, "menuTemplate": [ { "label": "View details", "title": "{type} {name}", "action": "contentDialog", "iconClass": "dijitIconTask" }, { "iconClass": "dijitIconConnector" }, { "iconClass": "dijitIconFilter" } ], "type": "JBrowse/View/Track/CanvasFeatures", "storeClass": "JBrowse/Store/SeqFeature/NCList", "label": "temptest1", "urlTemplate": "tracks/temptest1/{refseq}/trackData.json", "key": "temptest1", "trackType": "CanvasFeatures", "ItemRgb": true, "compress": 0, "baseUrl": " http://menash.md.huji.ac.il/millstone/jbrowse/data/dir-for-gianluca//", "index": 9, "metadata": {} } What are we doing wrong? Also we would also like to know if it is possible to save a session e.g. if tracks were manually edited can they all be saved with the new configurations? Thanks, Yael |
From: Colin <col...@gm...> - 2019-07-23 17:29:22
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It looks like maybe these annotations are in protein space. I guess what you are looking for is to convert this protein annotation into genome coordinates. This isn't something that is done by jbrowse tools. I would maybe ask the BRAKER team, as they probably work with this type of scenario often. -Colin On Tue, Jul 23, 2019 at 12:52 PM Lbl <nat...@lb...> wrote: > Cross posting to jbrowse list > > On Jul 23, 2019, at 12:17 AM, Michał T. Lorenc <m.t...@gm...> > wrote: > > Hi > Our annotation is based on BRAKER2 additionally I ran InterProScan and I > got GFF3, TSV and XML. I tried to load the GFF3 file using > > > *> perl /apollo/jbrowse/bin/flatfile-to-json.pl > <http://flatfile-to-json.pl> --gff > /apollo/braker-soft_utr-interproscan.gff3 --compress --trackType > HTMLFeatures --trackLabel "interproscan" --out /apollo/* > > It appears that no InterProScan results has been loaded. I assume that the > InterProScan's GFF3 file does not contain any chromosome names: > > > > > > > > > > > > > > > > > > > > *##gff-version 3##feature-ontology > http://song.cvs.sourceforge.net/viewvc/song/ontology/sofa.obo?revision=1.269 > <http://song.cvs.sourceforge.net/viewvc/song/ontology/sofa.obo?revision=1.269>##interproscan-version > 5.36-75.0##sequence-region g109343.t1 1 1358g109343.t1 . > polypeptide 1 1358 . + . > ID=g109343.t1;md5=3e908dc966fefe367e64dc9d98b0d3abg109343.t1 > ProSiteProfiles protein_match 628 724 18.261 + . > date=20-07-2019;Target=g109343.t1 628 > 724;Ontology_term="GO:0015074";ID=match$1_628_724;signature_desc=Integrase > catalytic domain > profile.;Name=PS50994;status=T;Dbxref="InterPro:IPR001584"g109343.t1 > SUPERFAMILY protein_match 586 624 4.19E-5 + . > date=20-07-2019;Target=g109343.t1 586 > 624;Ontology_term="GO:0003676","GO:0008270";ID=match$2_586_624;Name=SSF57756;status=T;Dbxref="InterPro:IPR036875"g109343.t1 > SUPERFAMILY protein_match 622 725 4.93E-29 + > . date=20-07-2019;Target=g109343.t1 622 > 725;ID=match$3_622_725;Name=SSF53098;status=T;Dbxref="InterPro:IPR012337"g109343.t1 > ProSiteProfiles protein_match 278 294 9.636 + . > date=20-07-2019;Target=g109343.t1 278 > 294;Ontology_term="GO:0003676","GO:0008270";ID=match$4_278_294;signature_desc=Zinc > finger CCHC-type > profile.;Name=PS50158;status=T;Dbxref="InterPro:IPR001878"g109343.t1 > SMART protein_match 600 616 0.36 + . > date=20-07-2019;Target=g109343.t1 600 > 616;Ontology_term="GO:0003676","GO:0008270";ID=match$5_600_616;Name=SM00343;status=T;Dbxref="InterPro:IPR001878"g109343.t1 > SMART protein_match 278 294 6.3E-4 + . > date=20-07-2019;Target=g109343.t1 278 > 294;Ontology_term="GO:0003676","GO:0008270";ID=match$5_278_294;Name=SM00343;status=T;Dbxref="InterPro:IPR001878"g109343.t1 > Pfam protein_match 82 216 1.2E-24 + . > date=20-07-2019;Target=g109343.t1 82 > 216;ID=match$6_82_216;signature_desc=gag-polypeptide of LTR > copia-type;Name=PF14223;status=T* > > Is there a solution how to load InterProScan results as a track into > JBrowse or are there any scripts which combine InterProScan with BRAKER2? > > Thank you in advance, > > Michal > > -- > To unsubscribe from this group and stop receiving emails from it, send an > email to apo...@lb.... > |