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Genetic Lifeform and Disk Operating System, or GLaDOS for short, is an operating system for personal computers. Inspired by the fictional artificial intelligence GLaDOS from Valve’s Portal video game series, this operating system is a fully functional replica of the operating system from the games.

GLaDOS is based on FreeDOS 1.0, a free and open-source operating system developed as a replacement for Microsoft DOS (MS-DOS), and both FreeDOS and GLaDOS can run programs intended for MS-DOS. Popular programs such as Fallout 1, Microsoft Word, Doom, Lotus 1-2-3, and more run flawlessly under GLaDOS without emulation.

GLaDOS uses several components of the FreeDOS project, as well as other utilities and programs, to create a minimal operating system environment:

• 4DOS command line for improved command-line functionality
• XCDROM and SHSUCDX to add support for CD drives
• DOSLFN 0.41 to add Windows Long File Name support
• CuteMouse 2.1 Beta 4 for programs that require mouse

GLaDOS is capable of running on any Intel 80386 CPU (or higher) PC with 512KB of RAM, and is available as a bootable floppy image or a bootable CD.