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{\*\generator Msftedit 5.41.21.2510;}\viewkind4\uc1\pard\sa200\sl276\slmult1\lang9\f0\fs22 This website includes four primer design tools that we developed at Peccoud Lab (part of Virginia Bioinformatics Institute) and are now making available to the community at large.  They include:\par
\pard{\pntext\f2\'B7\tab}{\*\pn\pnlvlblt\pnf2\pnindent0{\pntxtb\'B7}}\fi-360\li720\sa200\sl276\slmult1 PCR Primer Design Tool: The PCR Primer Calculator creates PCR primers for user-defined sequences based on a set of configurable criteria. This tool will develop PCR Primers for up to eight sequences at a time.\par
{\pntext\f2\'B7\tab}PCR Primer Mass Generator: The PCR Primer Mass Generator creates PCR primers for user-defined sequences based on a set of configurable criteria. This tool accepts a Comma-Separated Value (csv) text file of sequences as input.\par
{\pntext\f2\'B7\tab}FISH Probe Set Design Tool: The Gene Primer Calculator creates oligo probe sets to tag the mRNA of specified genes for Fluorescence in situ hybridization (FISH).\par
{\pntext\f2\'B7\tab}Plasmid Primer Design Tool: The Plasmid Primer Calculator creates sequencing primers for plasmids based on a list of configurable criteria.\par
\pard\sa200\sl276\slmult1 These tools are copyrighted by the Peccoud Lab and may be accessed online at {\field{\*\fldinst{HYPERLINK "http://gladden.vbi.vt.edu/primerDesign"}}{\fldrslt{\ul\cf1 http://gladden.vbi.vt.edu/primerDesign}}}\f0\fs22  or installed locally.  \par
Installation is simple:\par
\pard{\pntext\f2\'B7\tab}{\*\pn\pnlvlblt\pnf2\pnindent0{\pntxtb\'B7}}\fi-360\li720\sa200\sl276\slmult1 Copy these files into a PHP-enabled webroot.\par
{\pntext\f2\'B7\tab}Download the Original Code for the BioPhp Minitools (available here: \lang1033\b\f1{\field{\*\fldinst{HYPERLINK "http://www.biophp.org/minitools/melting_temperature/index.php?action=o_code"}}{\fldrslt{\ul\cf1 http://www.biophp.org/minitools/melting_temperature/index.php?action=o_code}}}\lang9\b0\f0\fs22 ) and save it as biophp_minitools.php; place this file in the same directory with the other files.\par
{\pntext\f2\'B7\tab}Edit the common.php if you want to change some of the default settings.\par
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