#11773 NTR: MF terms for EC reactions 2.7.7.87, 2.3.1.234, 2.8.4.5

[Ingrid]

These are all enzymes involved in the threonylcarbamoyladenosine modification of tRNAs. I just ran across these again because GO:0070526 seems to have been recently obsoleted, and I looked at some of the proteins in E. coli that were annotated to that term.

Thanks,
Ingrid

[David Hill]

Hi Ingrid,

Here are the terms. Let me know if you need anything else.

[Term]
+id: GO:0061710
+name: L-threonylcarbamoyladenylate synthase
+namespace: molecular_function
+def: "Catalysis of the reaction: L-threonine + ATP + bicarbonate = L-threonylcarbamoyladenylate + diphosphate + H(2)O." [EC:2.7.7.87]
+xref: EC:2.7.7.87
+xref: MetaCyc:RXN-14569
+xref: RHEA:36410
+is_a: GO:0016779 ! nucleotidyltransferase activity
+created_by: dph
+creation_date: 2015-06-23T08:25:14Z
+
+[Term]
+id: GO:0061711
+name: N(6)-L-threonylcarbamoyladenine synthase
+namespace: molecular_function
+def: "Catalysis of the reaction: L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA." [EC:2.3.1.234]
+xref: EC:2.3.1.234
+xref: MetaCyc:RXN-14570
+xref: RHEA:37062
+is_a: GO:0016747 ! transferase activity, transferring acyl groups other than amino-acyl groups
+created_by: dph
+creation_date: 2015-06-23T08:34:44Z
+
+[Term]
+id: GO:0061712
+name: tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase
+namespace: molecular_function
+def: "Catalysis of the reaction: N(6)-L-threonylcarbamoyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-L-threonylcarbamoyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine." [EC:2.8.4.5]
+xref: EC:2.8.4.5
+xref: MetaCyc:RXN-14531
+xref: RHEA:37078
+is_a: GO:0050497 ! transferase activity, transferring alkylthio groups
+created_by: dph
+creation_date: 2015-06-23T08:47:10Z
+

-David