We have updated Fijiwings for Mac (Fijiwings2.2) to coincide with our recent publication (Shipman, A. and Dobens, L.L. Using Fijiwings to understand the genetic control of cell growth and proliferation: a computer-based laboratory exercise. In press, Genetics Society of America Peer-Reviewed Education Portal (GSA PREP), 2015. http://www.genetics-gsa.org/education/GSAPREP.2015.003.shtml).
Two key updates have occurred. First, we went through the code and changed it to work properly with Weka segmentation analysis updates. Another small change (8-bit conversion) was required for reasons unclear to us, but now the program works with the current version of Fiji.
Separately, we found a discussion group entry leading us to believe that a pause in the macro after segmentation analysis was launched would be sufficient to make this tool stable and that seems to be the case. By adding "wait(1000)" after "run("Trainable Weka Segmentation");"
the macro pauses 1000 msec which allows Weka to launch. This simple change allows the tool to be used on 4GB RAM computers very stably, even with many programs running in the background. We have not tested it on 2GB RAM, because those are hard to find, but this is a great update and erases all the caveats we have attached to the program! No error messages are thrown.
We hope these changes help new and previous users of Fijiwings!
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We have updated Fijiwings for Mac (Fijiwings2.2) to coincide with our recent publication (Shipman, A. and Dobens, L.L. Using Fijiwings to understand the genetic control of cell growth and proliferation: a computer-based laboratory exercise. In press, Genetics Society of America Peer-Reviewed Education Portal (GSA PREP), 2015. http://www.genetics-gsa.org/education/GSAPREP.2015.003.shtml).
Two key updates have occurred. First, we went through the code and changed it to work properly with Weka segmentation analysis updates. Another small change (8-bit conversion) was required for reasons unclear to us, but now the program works with the current version of Fiji.
Separately, we found a discussion group entry leading us to believe that a pause in the macro after segmentation analysis was launched would be sufficient to make this tool stable and that seems to be the case. By adding "wait(1000)" after "run("Trainable Weka Segmentation");"
run("Trainable Weka Segmentation"); wait (1000); *the macro pauses 1000 msec which allows Weka to launch. This simple change allows the tool to be used on 4GB RAM computers very stably, even with many programs running in the background. We have not tested it on 2GB RAM, because those are hard to find, but this is a great update and erases all the caveats we have attached to the program! No error messages are thrown.
We hope these changes help new and previous users of Fijiwings!