gatting for serval files with distinc range for the same parameter

[Anonymous]

Hie
I find very use full your FCSalyzer software. unfortunatly I work on a Novocyte and the FCS files exported Have not the same range. Values are similar but range not so when I dotplot for gating the gate stay but the scale move so I can't select the good populations.
I try the altenative 0.9.22-alpha but i doesn't do this job also if I plot overlay.
Is there a solution to use whole range for whole files ?
the atter exist on serval mesure :FSC/SSC PE/APC and FITC/PE-Cy7
for example to file nearly withou difference on FITC witch may show a big translation.
thanks a lot
wery good work
pierre

[Sven Mostböck]

Hi Pierre,

so Novocyte FCS files have different ranges within one experiment? OK, I was not aware that this can happen for some machines.

Currently there is no way to manually change the range, sorry.
I plan to work more on the program this winter. There are a number of things I want to improve, and I can add an option to pre-set the range.
However, this will take some time, it is not a quick fix.

Say, could you maybe upload two FCS files with different ranges, so I have some test cases?

Thanks,
Sven

[Anonymous]

Hie Sven
"so Novocyte FCS files have different ranges within one experiment? OK, I was not aware that this can happen for some machines."
=> Egal, I was very disapoited when I try to analyze ;)

I tryed to join the files in withe the image but it doesn't pass, I'll try again or send by other way.
Thanks a lot
I realy like your software, litle, simple, usefull and can read my data. Unfortunatly Y have too strange file description
best regards
pierre