We introduce four novel assembly quality metrics that assess the suitability of an assembly for genomic analysis of TEs.
In particular we ask if piRNA clusters are contiguously assembled (CUSCO) and if an assembly accurately captures the TE landscape of an organisms (three TE landscape metrics). Here we provide a manual for each script and a walkthrough.
Wierzbicki F, Schwarz F, Cannalonga O, Kofler R. Novel quality metrics allow identifying and generating high-quality assemblies of piRNA clusters. Mol Ecol Resour. 2022,22,102-121. doi:10.1111/1755-0998.13455
Filip Wierzbicki and Florian Schwarz contributed equally to this work.
Wiki: CUSCO
Wiki: FlankDesign
Wiki: Manual
Wiki: TELandscapeMetrics
Wiki: piRNAclusterAlign