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Robert Kofler Filip Wierzbicki

Introduction

We introduce four novel assembly quality metrics that assess the suitability of an assembly for genomic analysis of TEs.
In particular we ask if piRNA clusters are contiguously assembled (CUSCO) and if an assembly accurately captures the TE landscape of an organisms (three TE landscape metrics). Here we provide a manual for each script and a walkthrough.

Manual

  • [Manual] explanation of the scripts and of the parameters

Walkthrough

Citation

Wierzbicki F, Schwarz F, Cannalonga O, Kofler R. Novel quality metrics allow identifying and generating high-quality assemblies of piRNA clusters. Mol Ecol Resour. 2022,22,102-121. doi:10.1111/1755-0998.13455

Filip Wierzbicki and Florian Schwarz contributed equally to this work.

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Related

Wiki: CUSCO
Wiki: FlankDesign
Wiki: Manual
Wiki: TELandscapeMetrics
Wiki: piRNAclusterAlign