I am trying to obtain both NSAF and dNSAF values from an experiment originally run in scaffold. I have tried exporting files to both pepxml format and mzid format and then run crux spectral-counts. When running pepxml, I have specified the protein fasta file database. With mzid, I have not. Either way I have tried running the program I get an initial set of errors stating "No sequence found..." followed by info:number of matches 309393 Info:number of matches passed the threshold 309393 "FATAL: An exception occurred: invalid vector[t] subscript". I am guessing that I have not done some required step, but I was unable to find any information on this in the tutorial or in the previous issues. Thank you for your help.
Hi Christopher,
Sorry to hear that this is not working right. Would you mind posting some sample files, along with command lines, so that we can reproduce and then fix this problem?
Bill