Description:
Comet assay provides an easy solution to estimate DNA damage in single cells through microscopy assessment. To obtain reproducible and reliable quantitative data, we developed an easy-to-use tool named CometAnalyser. CometAnalyser is an open-source deep-learning tool designed for the analysis of both fluorescent and silver-stained wide-field microscopy images. Once the comets are segmented and classified, several intensity/morphological features are automatically exported as a spreadsheet file.
Video Tutorial:
CometAnalyser is written in MATLAB. It works with Windows, Macintosh, and UNIX-based systems. Please, download the sample datasets and test it watching the video tutorial to understand how it works:
https://www.youtube.com/watch?v=vh2VFnMw50A
Contacts:
filippo.piccinini85@gmail.com
beleonattila@gmail.com
Features
- MATLAB
- Open Source
- Comet Assay
- Silver stained images
- Fluorescence images
- Quantitative analysis