#7 CIRI's coding

[Johann]

Hi,

I understand that CIRI (or CIRI2 for that matter) is able to add details of gene ID if we provide an annotation file (.gtf). What I notice is that adding an annotation file seems to give me more hits in the output than not adding an annotation file; from my last trial, it was about 16 hits more in the former.

May I know whether this is expected? Thank you.

[Yuan Gao]

Hi Johann,

CIRI not only adds details of gene ID according to the annotation provided, but can also use exon ends in annotation to search potential circRNAs with no GT-AG splicing signals. So usually more circRNAs are found when annotation is provided.

Thanks,
Yuan