#15 In all my dataset samples I have no detect any Circular RNA
Dear Author of Article: CIRI: an efficient and unbiased algorithm for de novo circular RNA identification.
I downloaded CIRI2 and for running this tool based on my RNA-seq dataset which they are control access. as an example, I execute the below command:
perl CIRI2.pl -I SRR1176949.sam -O outfile6949 -F wg.fa
but for all samples in my dataset, same as the below report for SRR1176949.sam, I couldn't detect any circular RNA. I don't think this condition would be normal.
I appreciate it if you share your comment with me.
Best Regards,
Mohammad
[Tue Jan 19 23:28:22 2021] CIRI begins running
[Tue Jan 19 23:28:22 2021] Loading reference
[Tue Jan 19 23:29:02 2021] First scanning
Worker 1 begins to scan SRR1176949.sam.
Worker 1 finished reporting.
Candidate reads with splicing signals: 0
Candidate reads with PEM signals: 0
Candidate circRNAs found: 0
[Tue Jan 19 23:42:17 2021] Second scanning
Worker 2 begins to scan SRR1176949.sam.
Worker 2 finished reporting.
[Wed Jan 20 00:12:37 2021] Extracting info from temporary files
Additional candidate reads found: 0
Additional candidate reads with PEM signals: 0
[Wed Jan 20 00:13:37 2021] Summarizing
Number of circular RNAs found: 0
[Wed Jan 20 00:13:37 2021] CIRI finished its work. Please see output file outfile6949 for detail.
