Hi Yuan Gao,
I have been attempting to run CIRI in preparation for CIRI-AS on a HPC with increasing computational performance. Unforntunately, each time I run CIRI the program times out before the second scan begins.
The input files are paired-end human samples and the code (without pathnames) I am using is:
perl CIRI_v1.2.pl -P -I input.sam -O output.txt -F hg19.fa -A gencode.v19.annotation.gtf
Do you know why the program does not progress past second scanning?
Thank you kindly in advance,
Lachlan Gray
CIRI v1.2 needs more time and memory. Please use latest version v2.0.6 for your analysis. If the results will be further used for CIRI-AS, please make sure the read length is the same.