Tutorials

Cell Sampler

Tutorials

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• Cell Sampler
  • Tutorials
    • Description
    • Overview of the Sampling Process
    • Working Directories
    • Tutorial Material

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Description

This document contains links to two short tutorials on how to use the Cell Sampler plugin to obtain time series from microscope TIFF movies expressing the GFP or luciferase reporter molecules.

-> Tutorial Material

Overview of the Sampling Process

The sampling processing to get time series from a TIFF movie consists of the following basic steps.
Each of these basic steps is described in the Tutorial Material.

1. Starting ImageJ and the Cell Sampler plugin.
2. Opening the Image Stack in the plugin.
3. Placement of ROI on the images, specifying the sampling coordinates for cellular features in a movie.
4. Saving the sampled ROI coordinates to file.
5. Exporting the time series obtained from cells in a TIFF movie to a spread-sheet.

Working Directories

When working with imaging data, it is recommended that you keep your imaging data files and output files from Cell Sampler in their own directories. This keeps the plugin's inputs and outputs separate. For each TIFF movie sampled by this plugin, try to create an output directory for the set of output files created by the plugin.

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Tutorial Material

Title	Description
[Basic Tutorial]	Reviews how to open TIFF movies and position Regions-Of-Interest (ROI) for sampling.
[Advanced Tutorial]	Reviews how to use the plugin to track cellular drift in a movie.