#2 Linux Illegal modulus zero

[Boahemaa Adu-Oppong]

Hello, I am getting this error message whenever I try to run the clustering with USEARCH: Illegal modulus zero at /scratch/gdlab/badu/shotgun_sequencing/raw_reads/Prokka_annotations/BPGA-1.3-linux-x86_64-0-0-0/lib/std/cavaexecscript/BPGA-Version-1.3.pl line 2192, <DATA> line 1.

There is no folder std/cavaexedscript; therefore, I can't check line 2192. The first part works fine with input preparation for clustering. I have four output files from that one: INPUT_all.fnn, INPUT_all.seq, list and gi_name. Thank you.

[Boahemaa Adu-Oppong]

Also, before clustering fails this is what is printed out:

./usearch -sortbylength INPUT_all.seq -fastaout INPUT_all_sorted.faa -minseqlength 20

---Fatal error---
Missing --output

[Narendrakumar Chaudhari]

Check if Input_all.seq is not empty. If all are files generated during peparation, relaunch BPGA and start from step 2.

[Boahemaa Adu-Oppong]

Thank you for the response. I'll go ahead and try that. Thank you.

[Codie]

I was receiving the same error. I followed Narendrakumar's advice and it worked for me!!

[Idowu Olawoye]

I am having the same error and restarting step 2 doesnt solve it. What can I do?
The input_all.seq file is not empty.

[Narendrakumar Chaudhari]

Then, close BPGA. Relaunch it and then directly run to step 2.