I was trying to build 3 new bowtie indexes with Macaca mulatta alone and
> with SIV isolates 239 and E543. The only files that were built were the 3
> and 4.ebwt files. I also received an error about the size of the reference
> sequence. We tried to build the index using the original unzipped fasta chromosome
> files and managed to get through 1-4.ebwt but stopped the program before
> allowing it to finish because we assumed it was working. When we tried to go
> back and use the same fasta files saved under a shorter name (chr1, chr2
> etc) we ran into the problem of only getting the 3 and 4 index files. The
> same happened when we tried to use a combined chromosome fasta file. I am
> including text from Terminal below. Any advice? Any chance you already have
> this index available?
> mac6:bowtie-0.12.7 mac6$ bowtie-build
> Output files: "mmul.*.ebwt"
> Line rate: 6 (line is 64 bytes)
> Lines per side: 1 (side is 64 bytes)
> Offset rate: 5 (one in 32)
> FTable chars: 10
> Strings: unpacked
> Max bucket size: default
> Max bucket size, sqrt multiplier: default
> Max bucket size, len divisor: 4
> Difference-cover sample period: 1024
> Endianness: little
> Actual local endianness: little
> Sanity checking: disabled
> Assertions: disabled
> Random seed: 0
> Sizeofs: void*:8, int:4, long:8, size_t:8
> Input files DNA, FASTA:
> Reading reference sizes
> Error: Reference sequence has more than 2^32-1 characters! Please divide
> reference into batches or chunks of about 3.6 billion characters or less
> and index each independently.
> Time reading reference sizes: 00:00:58
> Total time for call to driver() for forward index: 00:00:58
> Command: bowtie-build
Heya i am for the first time here. I found this board and I find It truly useful & it helped me out a lot. I hope to give something back and help others like you aided me.
<a href="Office 2010 Professional Key" title="http://www.office2010keyforsale.com/">http://www.office2010keyforsale.com/</a>
Great delivery. Great arguments. Keep up the good work.
<a href="http://www.getwindows7ultimatekey.com/" title="Windows 7 Professional Key">Windows 7 Professional Key</a>
Can you please email me the code for this script or please let know me in detail in relation to this script?
<a href="http://www.canadagoosejacketsales.com/" title="Click Here">Click Here</a>
http://www.bubbles-bags.com insanity http://insantiyworkoutuk.webeden.co.uk insanity
Log in to post a comment.