Welcome

This tool was designed to allow common researchers with common informatic resources (a standard pc running Windows XP, Vista was not tested) to compare a set of ORFs with a given reference set of annotated ORFs. The sets can range from a few FASTA encoded sequences to the full ORF complement of a given genome. Each query sequence will be checked (‘blasted’) against every reference sequence and back (bi-directional BLAST) in order to detect putative orthologue genes. Subsequently every pair of matching sequences, detected either in just one or both directions will be refined by global alignment and relevant statistics will be collected about each match. The process ends with GO ontology assignment and a tentative clustering of putative paralogue genes. The analysis can be stopped at any level and the relevant parameters are fully customisable.

Files (screenshots and sample results) will be made available very soon. The code and executables will follow, as the core of the tool is almost finished. The user interface is rudimental still and far from perfect. So the first release will be fully functional but will probably suffer from poor ui interactivity.