Hey all,
First of all I have to say that I really appreciate all the work that has been done on BBmap, Thanks!
I wondered if there is any option of adding a flag to allow mismatches in the indices sequences while demultiplexing.
When looking into it I noticed that I can retreive many reads that have only one mismatch in one of indices and that's a shame throwing them away.
When designing and choosing my indices I make sure the hamming distance is large enough so I am not concerned by accidently classifying reads to wrong samples
Thanks !
Shahar
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Hey all,
First of all I have to say that I really appreciate all the work that has been done on BBmap, Thanks!
I wondered if there is any option of adding a flag to allow mismatches in the indices sequences while demultiplexing.
When looking into it I noticed that I can retreive many reads that have only one mismatch in one of indices and that's a shame throwing them away.
When designing and choosing my indices I make sure the hamming distance is large enough so I am not concerned by accidently classifying reads to wrong samples
Thanks !
Shahar