From: Dan B. <dan...@gm...> - 2009-05-27 18:29:03
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Hi, I'm running an assembly using the Celera Assembler on a large set of 454 reads. The assembly seems to run fine, but in the output (in hawkeye) I'm seeing several scaffolds. I was wondering why there are any scaffolds in the data, as the reads are all single end with no mate-pair information. Could my <file>.seq / <file>.qual import pipeline be leading to incorrect identification of libraries and mate pairs? Sorry for the basic question, but I just don't see why scaffolds are being produced otherwise. Cheers, Dan. |