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PCR primers / probes design from multiple & degenerate sequences
... PCR (qPCR, rt-PCR, etc.), and Sanger sequencing. Gemi can parse large dataset of sequences efficiently.
Article:
Gemi: PCR primers prediction from multiple alignments
Comparative and functional genomics 2012;2012:783138
DOI: https://doi.org/10.1155/2012/783138
PMID: https://www.ncbi.nlm.nih.gov/pubmed/23316117
PMCID: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3535827/
Deconvolutes qPCR data to estimate cell-type-specific gene expression
DEBay: Deconvolution of Ensemble through Bayes-approach
DEBay estimates cell type-specific gene expression by deconvolution of quantitative PCR data of a mixed population. It will be useful in experiments where the segregation of different cell types in a sample is arduous, but the proportion of different cell types in the sample can be measured. DEBay uses the population distribution data and the qPCR data to calculate the relative expression of the target gene in different cell types...