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  • 1
    DEBay

    DEBay

    Deconvolutes qPCR data to estimate cell-type-specific gene expression

    ...The user manual of DEBay: https://sourceforge.net/projects/debay/files/UserManual.pdf Sample data: https://sourceforge.net/projects/debay/files/Test_data/ Citation Information: Vimalathithan Devaraj, Biplab Bose. DEBay: A computational tool for deconvolution of quantitative PCR data for estimation of cell type-specific gene expression in a mixed population. Heliyon, 2020, 6(7), e04489. https://doi.org/10.1016/j.heliyon.2020.e04489
    Downloads: 1 This Week
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  • 2

    PrimerSeq

    Designs primers on flanking constitutive exons for RT-PCR

    Downloads: 3 This Week
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  • 3
    pyQPCR
    pyQPCR is a GUI application written in python that deals with quantitative PCR (QPCR) raw data. Using quantification cycle values extracted from QPCR instruments, it uses a proven and universally applicable model to give finalized quantification resu
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    Downloads: 1 This Week
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  • 4

    InMethyl

    Design of target-specific primers for bis. sequencing of CpG-islands

    InMethyl is a Python-based application for the design of target-specific primer combinations for PCR amplification and bisulfite sequencing of complete CpG-islands. InMethyl uses bowtie high-throughoutput aligner to identify potential mis-priming sites in the bisulfite treated or intact genome and then – undesirable potential PCR products. InMethyl provides a balance between various characteristics that allows to pick up primers in hard-to-study genomic regions.
    Downloads: 0 This Week
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  • 5

    Primer Design Tool

    Design PCR primers

    A simple tool to design DNA primers for mutagenesis in BGME lab (JHU). Allows users to select or enter background sequence and add current and new mutations. During the design process, users are presented with various updating checks to guide them.
    Downloads: 0 This Week
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  • 6
    Pythia is a thermodynamically based PCR primer design program.
    Downloads: 0 This Week
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  • 7
    Amplicon is a Python script for designing PCR primer sets. Amplicon is written in Python 2.3 and Tkinter 8.4. The current script was created for Windows and an executable is available. Future versions of the script should be able to run on Linux and Mac
    Downloads: 2 This Week
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