Gemi
PCR primers / probes design from multiple & degenerate sequences
... (qPCR, rt-PCR, etc.), and Sanger sequencing. Gemi can parse large dataset of sequences efficiently.
Article:
Gemi: PCR primers prediction from multiple alignments
Comparative and functional genomics 2012;2012:783138
DOI: https://doi.org/10.1155/2012/783138
PMID: https://www.ncbi.nlm.nih.gov/pubmed/23316117
PMCID: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3535827/