Automatic design of primers for miR-specific RT-qPCR
...Quantification of miRNAs by a simple and specific qPCR method. Methods in Molecular Biology. 1182, 73-81.).
The program was written in Ruby and is available as source code for developers and as an .exe file for easy use.
Framework for making Windows applications that are one .exe file in AutoHotKey_L,C++,C#, VB.NET,Java,Groovy,Common Lisp,Nemerle,Ruby,Python,PHP,Lua,Tcl,Perl,Jint,S#,WSH VBScript,HTML/JavaScript/CSS,COM, PowerShell without compiling . For .NET 4.