Build VMs, containers, AI, databases, storage—all in one place.
Start your project in minutes. After credits run out, 20+ products include free monthly usage. Only pay when you're ready to scale.
Get Started
Earn up to 16% annual interest with Nexo.
Let your crypto work for you
Put idle assets to work with competitive interest rates, borrow without selling, and trade with precision. All in one platform.
Geographic restrictions, eligibility, and terms apply.
GUI for fitting 3D single-molecule images using the double-helix PSF
...Version 2 also includes code that facilitates combining localization data from two spectral channels using a locally-weighted quadratic 3D registration function
Features
- Double-Gaussian estimator is calibrated via an axial scan of bright immobile fluorescent emitters (e.g. beads)
- DH localization is performed through template matching and subsequent double Gaussian fitting
- Tiff stacks of SM images are analyzed using template matching followed by double-Gaussian fitting to extract estimates of the molecule positions
- Two spectral channels are registered by generating a locally-weighted quadratic mapping function from control point pairs
This package includes a collection of MATLAB files which are designed to:
1. Given a calibration scan of the image of a point emitter with an engineered point spread function (PSF),
2. Perform a phase retrieval algorithm based on maximum likelihood estimation (MLE) of a phase aberration term which is added to the theoretical pupil function of the imaging system.
3. Use the phase-retrieved pupil function to perform single-emitter localization.
Java Data Processing Project aims to manipulate text file containing scientific data such as time/intensity scan or spectrum. Jdpp provide large flexibility to read all possible time duration and date format. It is originally design to treat MS data.