Search Results for "double-entry"

...It's not necessary to have Matlab installed on your computer if you want to use the standalone app (Download button). You can find a documentation in the wiki tab. The program is freshly released. So feedback is very welcome in order to improve it. You can write a discussion entry in the wiki tab or write me a mail to elias.hils@uni.kn

This package of MATLAB routines with accompanying GUI allows scientific users to measure the 3D location of single molecules when using the double-helix point spread function (DH-PSF) widefield microscope. Version 2 also includes code that facilitates combining localization data from two spectral channels using a locally-weighted quadratic 3D registration function Features - Double-Gaussian estimator is calibrated via an axial scan of bright immobile fluorescent emitters (e.g. beads) - DH localization is performed through template matching and subsequent double Gaussian fitting - Tiff stacks of SM images are analyzed using template matching followed by double-Gaussian fitting to extract estimates of the molecule positions - Two spectral channels are registered by generating a locally-weighted quadratic mapping function from control point pairs