Enhanced splice junction detection and estimation from RNA-Seq data
...Multiple mapping reads with a unique location after filtering are rescued and reallocated to the most reliable candidate location.
FineSplice requires Python 2.x (>= 2.6) with the following modules installed: pysam (http://code.google.com/p/pysam/) and scikit-learn (http://scikit-learn.org/).
For further details check out our publication: Nucl. Acids Res. (2014) doi: 10.1093/nar/gku166