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PCR primers / probes design from multiple & degenerate sequences
...Article
Gemi: PCR primers prediction from multiple alignments. Comparative and functional genomics 2012
PMID: https://www.ncbi.nlm.nih.gov/pubmed/23316117
A great review on designing primer, Gemi, and other tools: Designing degenerate primers: Overview, challenges, and computational methods. Methods in Microbiology. Vol 57, 2025, Pages 73-89; DOI: 10.1016/bs.mim.2025.01.002
OW.NET -- 1-Wire API port from Java (and J#) to C#. Use the Dallas 1-Wire and iButton chips with standard C# using a serial adapter (also CF.net) or the TMEX drivers on the Windows platform. No J# distribution installation required.